TY  - JOUR
AU  - Milenković, Ivan
AU  - Keča, Nenad
AU  - Karadžić, Dragan
AU  - Nowakowska, Justyna A.
AU  - Oszako, Tomasz
AU  - Sikora, Katarzyna
AU  - Tkaczyk, Milosz
PY  - 2018
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/911
AB  - Common pathogenicity tests on 16-month-old ash plants were performed using isolates of Hymenoscyphus fraxineus and three different Phytophthora spp. isolated under declining ash trees in Wolica Nature reserve in Poland. There were no statistically significant differences among the different inoculation treatments and lesion lengths averaged 1.67 cm. After analyses of inoculated roots, five out of 10 root parameters showed statistically significant differences, and after a Dun can's Multiple Range test, significant differences were found with control plants while there were no differences among the treatments. The largest root loss was with the treatment inoculated with both H fraxineus and P. plurivora (HF + PP), and both fine and total root lengths were 2.30 and 2.27 times smaller, respectively, than the non-inoculated control. The earliest mortality of young ash plants was observed also with the I IF + PP treatment. Results showed high aggressiveness of IL fraxineus to young ash plants, with indications that root infections with Phytophthora spp. may contribute to greater damage, but more research is needed to clarify the interaction between H. fraxineus and Phytophthora spp. in ash dieback.
T2  - Forestry Chronicle
T1  - Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings
EP  - 139
IS  - 2
SP  - 135
VL  - 94
DO  - 10.5558/tfc2018-020
UR  - conv_1338
ER  - 

@article{
author = "Milenković, Ivan and Keča, Nenad and Karadžić, Dragan and Nowakowska, Justyna A. and Oszako, Tomasz and Sikora, Katarzyna and Tkaczyk, Milosz",
year = "2018",
abstract = "Common pathogenicity tests on 16-month-old ash plants were performed using isolates of Hymenoscyphus fraxineus and three different Phytophthora spp. isolated under declining ash trees in Wolica Nature reserve in Poland. There were no statistically significant differences among the different inoculation treatments and lesion lengths averaged 1.67 cm. After analyses of inoculated roots, five out of 10 root parameters showed statistically significant differences, and after a Dun can's Multiple Range test, significant differences were found with control plants while there were no differences among the treatments. The largest root loss was with the treatment inoculated with both H fraxineus and P. plurivora (HF + PP), and both fine and total root lengths were 2.30 and 2.27 times smaller, respectively, than the non-inoculated control. The earliest mortality of young ash plants was observed also with the I IF + PP treatment. Results showed high aggressiveness of IL fraxineus to young ash plants, with indications that root infections with Phytophthora spp. may contribute to greater damage, but more research is needed to clarify the interaction between H. fraxineus and Phytophthora spp. in ash dieback.",
journal = "Forestry Chronicle",
title = "Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings",
pages = "139-135",
number = "2",
volume = "94",
doi = "10.5558/tfc2018-020",
url = "conv_1338"
}

Milenković, I., Keča, N., Karadžić, D., Nowakowska, J. A., Oszako, T., Sikora, K.,& Tkaczyk, M.. (2018). Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings. in Forestry Chronicle, 94(2), 135-139.
https://doi.org/10.5558/tfc2018-020
conv_1338

Milenković I, Keča N, Karadžić D, Nowakowska JA, Oszako T, Sikora K, Tkaczyk M. Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings. in Forestry Chronicle. 2018;94(2):135-139.
doi:10.5558/tfc2018-020
conv_1338 .

Milenković, Ivan, Keča, Nenad, Karadžić, Dragan, Nowakowska, Justyna A., Oszako, Tomasz, Sikora, Katarzyna, Tkaczyk, Milosz, "Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings" in Forestry Chronicle, 94, no. 2 (2018):135-139,
https://doi.org/10.5558/tfc2018-020 .,
conv_1338 .

TY  - JOUR
AU  - Pacia, Artur
AU  - Nowakowska, Justyna A.
AU  - Tkaczyk, Milosz
AU  - Sikora, Katarzyna
AU  - Tereba, Anna
AU  - Borys, Malgorzata
AU  - Milenković, Ivan
AU  - Pszczolkowska, Agnieszka
AU  - Okorski, Adam
AU  - Oszako, Tomasz
PY  - 2017
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/833
AB  - The ash stand in Wolica reserve (Poland), affected with ash dieback, was studied. Isolations performed from collected ash tissues and rhizosphere soil samples revealed 28 isolates of Hymenoscyphus fraxineus and 27 isolates of Phytophthora spp., respectively. The vitality and defoliation of 198 and 176 trees were studied, respectively in 2012 and 2013. In 2013 only one tree was completely vital, while 83 trees were within the degeneration phase. These results suggested that trees being classified in this class are the most vital and the natural genetic resistance should be sought among this vitality class in the future. In total, 112 trees were classified to the stage 2 of vitality, for which large deformation of shoots is typical. Further, monitoring of defoliation in 2013 revealed that the largest number of trees in the stand (126) were moderately damaged trees (defoliation 26-60%), while 47 trees had over 60% of defoliation. The synthetic damage index was 1.58 in 2012 and 1.66 in 2013 indicating that advanced disease processes are occurring in this stand. In addition, sampling, isolation, morphological and molecular identifications of Phytophthora species were performed. After the isolation tests, P. megasperma, P. sp. hungarica, and P. plurivora were obtained. These results were confirmed after the PCR and ITS sequencing. This is the first report of P. sp. hungarica and P. megasperma in the stands of common ash in Poland. The natural genetic variation of the Fraxinus excelsior genome was studied to improve understanding of its role in the adaptation and tolerance processes facing ash dieback phenomenon. Six nuclear microsatellite markers and four chloroplast microsatellite markers have been used in order to assess the genetic diversity of Fraxinus excelsior stand in Poland, categorized into three different Roloff' classes of vitality 0+1, 2 and 3. We demonstrated lack of correlation between three different vitality classes of ash trees and their nuclear or chloroplast genetic differentiation. Nevertheless, the observed heterozygosity (H-O) value was significantly different between vitality classes 2 and 3 assessed with nuclear SSR markers (p = 0.000183 in HSD Tukey test, p  lt  0.05). Also private (Ap) alleles distribution of chloroplast SSR markers significantly differ (p = 0.000 in HSD Tukey test, p  lt  0.05) between the vitality classes 0+1 and 3 of ash trees. Those data suggest that DNA differentiation of F. excelsior at local spatial scale may be driven by gene based tolerance.
PB  - Lietuvos Misku Institutas
T2  - Baltic Forestry
T1  - Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland
EP  - 197
IS  - 1
SP  - 183
VL  - 23
UR  - conv_2043
ER  - 

@article{
author = "Pacia, Artur and Nowakowska, Justyna A. and Tkaczyk, Milosz and Sikora, Katarzyna and Tereba, Anna and Borys, Malgorzata and Milenković, Ivan and Pszczolkowska, Agnieszka and Okorski, Adam and Oszako, Tomasz",
year = "2017",
abstract = "The ash stand in Wolica reserve (Poland), affected with ash dieback, was studied. Isolations performed from collected ash tissues and rhizosphere soil samples revealed 28 isolates of Hymenoscyphus fraxineus and 27 isolates of Phytophthora spp., respectively. The vitality and defoliation of 198 and 176 trees were studied, respectively in 2012 and 2013. In 2013 only one tree was completely vital, while 83 trees were within the degeneration phase. These results suggested that trees being classified in this class are the most vital and the natural genetic resistance should be sought among this vitality class in the future. In total, 112 trees were classified to the stage 2 of vitality, for which large deformation of shoots is typical. Further, monitoring of defoliation in 2013 revealed that the largest number of trees in the stand (126) were moderately damaged trees (defoliation 26-60%), while 47 trees had over 60% of defoliation. The synthetic damage index was 1.58 in 2012 and 1.66 in 2013 indicating that advanced disease processes are occurring in this stand. In addition, sampling, isolation, morphological and molecular identifications of Phytophthora species were performed. After the isolation tests, P. megasperma, P. sp. hungarica, and P. plurivora were obtained. These results were confirmed after the PCR and ITS sequencing. This is the first report of P. sp. hungarica and P. megasperma in the stands of common ash in Poland. The natural genetic variation of the Fraxinus excelsior genome was studied to improve understanding of its role in the adaptation and tolerance processes facing ash dieback phenomenon. Six nuclear microsatellite markers and four chloroplast microsatellite markers have been used in order to assess the genetic diversity of Fraxinus excelsior stand in Poland, categorized into three different Roloff' classes of vitality 0+1, 2 and 3. We demonstrated lack of correlation between three different vitality classes of ash trees and their nuclear or chloroplast genetic differentiation. Nevertheless, the observed heterozygosity (H-O) value was significantly different between vitality classes 2 and 3 assessed with nuclear SSR markers (p = 0.000183 in HSD Tukey test, p  lt  0.05). Also private (Ap) alleles distribution of chloroplast SSR markers significantly differ (p = 0.000 in HSD Tukey test, p  lt  0.05) between the vitality classes 0+1 and 3 of ash trees. Those data suggest that DNA differentiation of F. excelsior at local spatial scale may be driven by gene based tolerance.",
publisher = "Lietuvos Misku Institutas",
journal = "Baltic Forestry",
title = "Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland",
pages = "197-183",
number = "1",
volume = "23",
url = "conv_2043"
}

Pacia, A., Nowakowska, J. A., Tkaczyk, M., Sikora, K., Tereba, A., Borys, M., Milenković, I., Pszczolkowska, A., Okorski, A.,& Oszako, T.. (2017). Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland. in Baltic Forestry
Lietuvos Misku Institutas., 23(1), 183-197.
conv_2043

Pacia A, Nowakowska JA, Tkaczyk M, Sikora K, Tereba A, Borys M, Milenković I, Pszczolkowska A, Okorski A, Oszako T. Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland. in Baltic Forestry. 2017;23(1):183-197.
conv_2043 .

Pacia, Artur, Nowakowska, Justyna A., Tkaczyk, Milosz, Sikora, Katarzyna, Tereba, Anna, Borys, Malgorzata, Milenković, Ivan, Pszczolkowska, Agnieszka, Okorski, Adam, Oszako, Tomasz, "Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland" in Baltic Forestry, 23, no. 1 (2017):183-197,
conv_2043 .