TY  - JOUR
AU  - Tkaczyk, Milosz
AU  - Milenković, Ivan
AU  - Nowakowska, Justyna A.
AU  - Borys, Malgorzata
AU  - Kaluski, Tomasz
AU  - Gawlak, Magdalena
AU  - Czyz, Michal
AU  - Oszako, Tomasz
PY  - 2017
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/806
AB  - The following paper presents the results on the determination of the diversity of species from the Phytophthora genus occurring in the declining oak stands in Krotoszyn Plateau in Poland. From the 50s of the last century, significant deterioration of oak health was observed in these stands, and Phytophthora species were suggested as one of the factors of the decline. In order to determine the presence of pathogenic organisms from the Phytophthora genus in these stands, 180 rhizosphere soil samples from three forest districts throughout the Krotoszyn Plateau were collected and subjected to the isolation method. Phytophthora species were consistently isolated from all the sampled stands, and 194 isolates from 111 positive samples were obtained. However, 150 (77%) and 44 (23%) isolates originated from the samples taken under the symptomatic and asymptomatic trees, respectively. All the obtained isolates were morphologically classified using the light and scanning electron microscopy and divided into morphological groups. Genomic DNA was isolated from selected isolates representing each group, ITS regions were amplified and sequence analyses were performed. In total, four different Phytophthora species were detected, including P. cactorum, P. plurivora, P. quercina and P. europaea. The most often isolated species were P. cactorum and P. plurivora. This is the first report of P. europaea in oak stands in Poland.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau
EP  - 215
IS  - 1
SP  - 203
VL  - 49
DO  - 10.2298/GENSR1701203T
UR  - conv_1279
ER  - 

@article{
author = "Tkaczyk, Milosz and Milenković, Ivan and Nowakowska, Justyna A. and Borys, Malgorzata and Kaluski, Tomasz and Gawlak, Magdalena and Czyz, Michal and Oszako, Tomasz",
year = "2017",
abstract = "The following paper presents the results on the determination of the diversity of species from the Phytophthora genus occurring in the declining oak stands in Krotoszyn Plateau in Poland. From the 50s of the last century, significant deterioration of oak health was observed in these stands, and Phytophthora species were suggested as one of the factors of the decline. In order to determine the presence of pathogenic organisms from the Phytophthora genus in these stands, 180 rhizosphere soil samples from three forest districts throughout the Krotoszyn Plateau were collected and subjected to the isolation method. Phytophthora species were consistently isolated from all the sampled stands, and 194 isolates from 111 positive samples were obtained. However, 150 (77%) and 44 (23%) isolates originated from the samples taken under the symptomatic and asymptomatic trees, respectively. All the obtained isolates were morphologically classified using the light and scanning electron microscopy and divided into morphological groups. Genomic DNA was isolated from selected isolates representing each group, ITS regions were amplified and sequence analyses were performed. In total, four different Phytophthora species were detected, including P. cactorum, P. plurivora, P. quercina and P. europaea. The most often isolated species were P. cactorum and P. plurivora. This is the first report of P. europaea in oak stands in Poland.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau",
pages = "215-203",
number = "1",
volume = "49",
doi = "10.2298/GENSR1701203T",
url = "conv_1279"
}

Tkaczyk, M., Milenković, I., Nowakowska, J. A., Borys, M., Kaluski, T., Gawlak, M., Czyz, M.,& Oszako, T.. (2017). Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 49(1), 203-215.
https://doi.org/10.2298/GENSR1701203T
conv_1279

Tkaczyk M, Milenković I, Nowakowska JA, Borys M, Kaluski T, Gawlak M, Czyz M, Oszako T. Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau. in Genetika-Belgrade. 2017;49(1):203-215.
doi:10.2298/GENSR1701203T
conv_1279 .

Tkaczyk, Milosz, Milenković, Ivan, Nowakowska, Justyna A., Borys, Malgorzata, Kaluski, Tomasz, Gawlak, Magdalena, Czyz, Michal, Oszako, Tomasz, "Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau" in Genetika-Belgrade, 49, no. 1 (2017):203-215,
https://doi.org/10.2298/GENSR1701203T .,
conv_1279 .

TY  - JOUR
AU  - Pacia, Artur
AU  - Nowakowska, Justyna A.
AU  - Tkaczyk, Milosz
AU  - Sikora, Katarzyna
AU  - Tereba, Anna
AU  - Borys, Malgorzata
AU  - Milenković, Ivan
AU  - Pszczolkowska, Agnieszka
AU  - Okorski, Adam
AU  - Oszako, Tomasz
PY  - 2017
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/833
AB  - The ash stand in Wolica reserve (Poland), affected with ash dieback, was studied. Isolations performed from collected ash tissues and rhizosphere soil samples revealed 28 isolates of Hymenoscyphus fraxineus and 27 isolates of Phytophthora spp., respectively. The vitality and defoliation of 198 and 176 trees were studied, respectively in 2012 and 2013. In 2013 only one tree was completely vital, while 83 trees were within the degeneration phase. These results suggested that trees being classified in this class are the most vital and the natural genetic resistance should be sought among this vitality class in the future. In total, 112 trees were classified to the stage 2 of vitality, for which large deformation of shoots is typical. Further, monitoring of defoliation in 2013 revealed that the largest number of trees in the stand (126) were moderately damaged trees (defoliation 26-60%), while 47 trees had over 60% of defoliation. The synthetic damage index was 1.58 in 2012 and 1.66 in 2013 indicating that advanced disease processes are occurring in this stand. In addition, sampling, isolation, morphological and molecular identifications of Phytophthora species were performed. After the isolation tests, P. megasperma, P. sp. hungarica, and P. plurivora were obtained. These results were confirmed after the PCR and ITS sequencing. This is the first report of P. sp. hungarica and P. megasperma in the stands of common ash in Poland. The natural genetic variation of the Fraxinus excelsior genome was studied to improve understanding of its role in the adaptation and tolerance processes facing ash dieback phenomenon. Six nuclear microsatellite markers and four chloroplast microsatellite markers have been used in order to assess the genetic diversity of Fraxinus excelsior stand in Poland, categorized into three different Roloff' classes of vitality 0+1, 2 and 3. We demonstrated lack of correlation between three different vitality classes of ash trees and their nuclear or chloroplast genetic differentiation. Nevertheless, the observed heterozygosity (H-O) value was significantly different between vitality classes 2 and 3 assessed with nuclear SSR markers (p = 0.000183 in HSD Tukey test, p  lt  0.05). Also private (Ap) alleles distribution of chloroplast SSR markers significantly differ (p = 0.000 in HSD Tukey test, p  lt  0.05) between the vitality classes 0+1 and 3 of ash trees. Those data suggest that DNA differentiation of F. excelsior at local spatial scale may be driven by gene based tolerance.
PB  - Lietuvos Misku Institutas
T2  - Baltic Forestry
T1  - Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland
EP  - 197
IS  - 1
SP  - 183
VL  - 23
UR  - conv_2043
ER  - 

@article{
author = "Pacia, Artur and Nowakowska, Justyna A. and Tkaczyk, Milosz and Sikora, Katarzyna and Tereba, Anna and Borys, Malgorzata and Milenković, Ivan and Pszczolkowska, Agnieszka and Okorski, Adam and Oszako, Tomasz",
year = "2017",
abstract = "The ash stand in Wolica reserve (Poland), affected with ash dieback, was studied. Isolations performed from collected ash tissues and rhizosphere soil samples revealed 28 isolates of Hymenoscyphus fraxineus and 27 isolates of Phytophthora spp., respectively. The vitality and defoliation of 198 and 176 trees were studied, respectively in 2012 and 2013. In 2013 only one tree was completely vital, while 83 trees were within the degeneration phase. These results suggested that trees being classified in this class are the most vital and the natural genetic resistance should be sought among this vitality class in the future. In total, 112 trees were classified to the stage 2 of vitality, for which large deformation of shoots is typical. Further, monitoring of defoliation in 2013 revealed that the largest number of trees in the stand (126) were moderately damaged trees (defoliation 26-60%), while 47 trees had over 60% of defoliation. The synthetic damage index was 1.58 in 2012 and 1.66 in 2013 indicating that advanced disease processes are occurring in this stand. In addition, sampling, isolation, morphological and molecular identifications of Phytophthora species were performed. After the isolation tests, P. megasperma, P. sp. hungarica, and P. plurivora were obtained. These results were confirmed after the PCR and ITS sequencing. This is the first report of P. sp. hungarica and P. megasperma in the stands of common ash in Poland. The natural genetic variation of the Fraxinus excelsior genome was studied to improve understanding of its role in the adaptation and tolerance processes facing ash dieback phenomenon. Six nuclear microsatellite markers and four chloroplast microsatellite markers have been used in order to assess the genetic diversity of Fraxinus excelsior stand in Poland, categorized into three different Roloff' classes of vitality 0+1, 2 and 3. We demonstrated lack of correlation between three different vitality classes of ash trees and their nuclear or chloroplast genetic differentiation. Nevertheless, the observed heterozygosity (H-O) value was significantly different between vitality classes 2 and 3 assessed with nuclear SSR markers (p = 0.000183 in HSD Tukey test, p  lt  0.05). Also private (Ap) alleles distribution of chloroplast SSR markers significantly differ (p = 0.000 in HSD Tukey test, p  lt  0.05) between the vitality classes 0+1 and 3 of ash trees. Those data suggest that DNA differentiation of F. excelsior at local spatial scale may be driven by gene based tolerance.",
publisher = "Lietuvos Misku Institutas",
journal = "Baltic Forestry",
title = "Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland",
pages = "197-183",
number = "1",
volume = "23",
url = "conv_2043"
}

Pacia, A., Nowakowska, J. A., Tkaczyk, M., Sikora, K., Tereba, A., Borys, M., Milenković, I., Pszczolkowska, A., Okorski, A.,& Oszako, T.. (2017). Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland. in Baltic Forestry
Lietuvos Misku Institutas., 23(1), 183-197.
conv_2043

Pacia A, Nowakowska JA, Tkaczyk M, Sikora K, Tereba A, Borys M, Milenković I, Pszczolkowska A, Okorski A, Oszako T. Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland. in Baltic Forestry. 2017;23(1):183-197.
conv_2043 .

Pacia, Artur, Nowakowska, Justyna A., Tkaczyk, Milosz, Sikora, Katarzyna, Tereba, Anna, Borys, Malgorzata, Milenković, Ivan, Pszczolkowska, Agnieszka, Okorski, Adam, Oszako, Tomasz, "Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland" in Baltic Forestry, 23, no. 1 (2017):183-197,
conv_2043 .