TY  - JOUR
AU  - Lazarević, Jelica
AU  - Milanović, Slobodan
AU  - Seslija Jovanović, Darka
AU  - Janković-Tomanić, Milena
PY  - 2023
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1381
AB  - Temperature and food quality are the most important environmental factors determining the performance of herbivorous insects. The objective of our study was to evaluate the responses of the spongy moth (formerly known as the gypsy moth) [Lymantria dispar L. (Lepidoptera: Erebidae)] to simultaneous variation in these two factors. From hatching to the fourth instar, larvae were exposed to three temperatures (19 ?, 23 ?, and 28 ?) and fed four artificial diets that differed in protein (P) and carbohydrate (C) content. Within each temperature regime, the effects of the nutrient content (P+C) and ratio (P:C) on development duration, larval mass, growth rate, and activities of digestive proteases, carbohydrases, and lipase were examined. It was found that temperature and food quality had a significant effect on the fitness-related traits and digestive physiology of the larvae. The greatest mass and highest growth rate were obtained at 28 ? on a high-protein low-carbohydrate diet. A homeostatic increase in activity was observed for total protease, trypsin, and amylase in response to low substrate levels in the diet. A significant modulation of overall enzyme activities in response to 28 ? was detected only with a low diet quality. A decrease in the nutrient content and P:C ratio only affected the coordination of enzyme activities at 28 ?, as indicated by the significantly altered correlation matrices. Multiple linear regression analysis showed that variation in fitness traits in response to different rearing conditions could be explained by variation in digestion. Our results contribute to the understanding of the role of digestive enzymes in post-ingestive nutrient balancing.
T2  - Biomolecules
T1  - Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae
IS  - 5
VL  - 13
DO  - 10.3390/biom13050821
UR  - conv_1710
ER  - 

@article{
author = "Lazarević, Jelica and Milanović, Slobodan and Seslija Jovanović, Darka and Janković-Tomanić, Milena",
year = "2023",
abstract = "Temperature and food quality are the most important environmental factors determining the performance of herbivorous insects. The objective of our study was to evaluate the responses of the spongy moth (formerly known as the gypsy moth) [Lymantria dispar L. (Lepidoptera: Erebidae)] to simultaneous variation in these two factors. From hatching to the fourth instar, larvae were exposed to three temperatures (19 ?, 23 ?, and 28 ?) and fed four artificial diets that differed in protein (P) and carbohydrate (C) content. Within each temperature regime, the effects of the nutrient content (P+C) and ratio (P:C) on development duration, larval mass, growth rate, and activities of digestive proteases, carbohydrases, and lipase were examined. It was found that temperature and food quality had a significant effect on the fitness-related traits and digestive physiology of the larvae. The greatest mass and highest growth rate were obtained at 28 ? on a high-protein low-carbohydrate diet. A homeostatic increase in activity was observed for total protease, trypsin, and amylase in response to low substrate levels in the diet. A significant modulation of overall enzyme activities in response to 28 ? was detected only with a low diet quality. A decrease in the nutrient content and P:C ratio only affected the coordination of enzyme activities at 28 ?, as indicated by the significantly altered correlation matrices. Multiple linear regression analysis showed that variation in fitness traits in response to different rearing conditions could be explained by variation in digestion. Our results contribute to the understanding of the role of digestive enzymes in post-ingestive nutrient balancing.",
journal = "Biomolecules",
title = "Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae",
number = "5",
volume = "13",
doi = "10.3390/biom13050821",
url = "conv_1710"
}

Lazarević, J., Milanović, S., Seslija Jovanović, D.,& Janković-Tomanić, M.. (2023). Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae. in Biomolecules, 13(5).
https://doi.org/10.3390/biom13050821
conv_1710

Lazarević J, Milanović S, Seslija Jovanović D, Janković-Tomanić M. Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae. in Biomolecules. 2023;13(5).
doi:10.3390/biom13050821
conv_1710 .

Lazarević, Jelica, Milanović, Slobodan, Seslija Jovanović, Darka, Janković-Tomanić, Milena, "Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae" in Biomolecules, 13, no. 5 (2023),
https://doi.org/10.3390/biom13050821 .,
conv_1710 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Janković-Tomanić, Milena
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Milanović, Slobodan
AU  - Stojković, Biljana
PY  - 2017
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/858
AB  - The gypsy moth is a generalist insect pest with an extremely wide host range. Adaptive responses of digestive enzymes are important for the successful utilization of plant hosts that differ in the contents and ratios of constituent nutrients and allelochemicals. In the present study, we examined the responses of alpha-amylase, trypsin, and leucine aminopeptidase to two tree hosts (suitable oak, Quercus cerris, and unsuitable locust tree, Robinia pseudoacacia) in the fourth, fifth, and sixth instars of gypsy moth larvae originating from oak and locust tree forest populations (hereafter assigned as Quercus and Robinia populations, respectively). Gypsy moths from the Robinia forest had been adapting to this unsuitable host for more than 40 generations. To test for population-level host plant specialization, we applied a two-population x two-host experimental design. We compared the levels, developmental patterns, and plasticities of the activities of enzymes. The locust tree diet increased enzyme activity in the fourth instar and reduced activity in advanced instars of the Quercus larvae in comparison to the oak diet. These larvae also exhibited opposite developmental trajectories on the two hosts, i. e. activity increased on the oak diet and decreased on the locust tree diet with the progress of instar. Larvae of the Robinia population were characterized by reduced plasticity of enzyme activity and its developmental trajectories. In addition, elevated trypsin activity in response to an unsuitable host was observed in all instar larvae of the Robinia population, which demonstrated that Robinia larvae had an improved digestive performance than did Quercus larvae.
T2  - Entomological Science
T1  - Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)
EP  - 194
IS  - 1
SP  - 189
VL  - 20
DO  - 10.1111/ens.12250
UR  - conv_1252
ER  - 

@article{
author = "Lazarević, Jelica and Janković-Tomanić, Milena and Savković, Uroš and Đorđević, Mirko and Milanović, Slobodan and Stojković, Biljana",
year = "2017",
abstract = "The gypsy moth is a generalist insect pest with an extremely wide host range. Adaptive responses of digestive enzymes are important for the successful utilization of plant hosts that differ in the contents and ratios of constituent nutrients and allelochemicals. In the present study, we examined the responses of alpha-amylase, trypsin, and leucine aminopeptidase to two tree hosts (suitable oak, Quercus cerris, and unsuitable locust tree, Robinia pseudoacacia) in the fourth, fifth, and sixth instars of gypsy moth larvae originating from oak and locust tree forest populations (hereafter assigned as Quercus and Robinia populations, respectively). Gypsy moths from the Robinia forest had been adapting to this unsuitable host for more than 40 generations. To test for population-level host plant specialization, we applied a two-population x two-host experimental design. We compared the levels, developmental patterns, and plasticities of the activities of enzymes. The locust tree diet increased enzyme activity in the fourth instar and reduced activity in advanced instars of the Quercus larvae in comparison to the oak diet. These larvae also exhibited opposite developmental trajectories on the two hosts, i. e. activity increased on the oak diet and decreased on the locust tree diet with the progress of instar. Larvae of the Robinia population were characterized by reduced plasticity of enzyme activity and its developmental trajectories. In addition, elevated trypsin activity in response to an unsuitable host was observed in all instar larvae of the Robinia population, which demonstrated that Robinia larvae had an improved digestive performance than did Quercus larvae.",
journal = "Entomological Science",
title = "Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)",
pages = "194-189",
number = "1",
volume = "20",
doi = "10.1111/ens.12250",
url = "conv_1252"
}

Lazarević, J., Janković-Tomanić, M., Savković, U., Đorđević, M., Milanović, S.,& Stojković, B.. (2017). Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae). in Entomological Science, 20(1), 189-194.
https://doi.org/10.1111/ens.12250
conv_1252

Lazarević J, Janković-Tomanić M, Savković U, Đorđević M, Milanović S, Stojković B. Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae). in Entomological Science. 2017;20(1):189-194.
doi:10.1111/ens.12250
conv_1252 .

Lazarević, Jelica, Janković-Tomanić, Milena, Savković, Uroš, Đorđević, Mirko, Milanović, Slobodan, Stojković, Biljana, "Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)" in Entomological Science, 20, no. 1 (2017):189-194,
https://doi.org/10.1111/ens.12250 .,
conv_1252 .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Janković-Tomanić, Milena
AU  - Kostić, Igor
AU  - Kostić, Miroslav
AU  - Morina, Filis
AU  - Zivanović, Bojana
AU  - Lazarević, Jelica
PY  - 2016
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/741
AB  - Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.
T2  - Entomologia Experimentalis Et Applicata
T1  - Behavioural and physiological plasticity of gypsy moth larvae to host plant switching
EP  - 162
IS  - 2
SP  - 152
VL  - 158
DO  - 10.1111/eea.12388
UR  - conv_1197
ER  - 

@article{
author = "Milanović, Slobodan and Janković-Tomanić, Milena and Kostić, Igor and Kostić, Miroslav and Morina, Filis and Zivanović, Bojana and Lazarević, Jelica",
year = "2016",
abstract = "Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.",
journal = "Entomologia Experimentalis Et Applicata",
title = "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching",
pages = "162-152",
number = "2",
volume = "158",
doi = "10.1111/eea.12388",
url = "conv_1197"
}

Milanović, S., Janković-Tomanić, M., Kostić, I., Kostić, M., Morina, F., Zivanović, B.,& Lazarević, J.. (2016). Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata, 158(2), 152-162.
https://doi.org/10.1111/eea.12388
conv_1197

Milanović S, Janković-Tomanić M, Kostić I, Kostić M, Morina F, Zivanović B, Lazarević J. Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata. 2016;158(2):152-162.
doi:10.1111/eea.12388
conv_1197 .

Milanović, Slobodan, Janković-Tomanić, Milena, Kostić, Igor, Kostić, Miroslav, Morina, Filis, Zivanović, Bojana, Lazarević, Jelica, "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching" in Entomologia Experimentalis Et Applicata, 158, no. 2 (2016):152-162,
https://doi.org/10.1111/eea.12388 .,
conv_1197 .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Mihajlović, Ljubodrag
AU  - Karadžić, Dragan
AU  - Jankovsky, Libor
AU  - Aleksić, Predrag
AU  - Janković-Tomanić, Milena
AU  - Lazarević, Jelica
PY  - 2014
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/569
AB  - Gypsy moths and powdery mildew play a significant role in oak decline processes. However, information is lacking on the effects on the gypsy moth of impaired tree vitality caused by defoliation or parasite infection. We assessed how pedunculate oak leaves collected from vigorous, declining, and infected trees influenced gypsy moth preference and performance (growth and nutritional indices). We found a negative effect of powdery mildew-infected leaves on gypsy moth performance, while declining trees had positive effects on gypsy moth performance and preference. All examined parameters of larvae fed declining oak leaves were higher than those of larvae fed vigorous oak leaves. Increased growth on declining oak leaves was caused by both higher consumption and more efficient food utilization. The results of this research could help us to better understand multitrophic interactions in complex communities such as oak forests.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effects of pedunculate oak tree vitality on gypsy moth preference and performance
EP  - 1672
IS  - 4
SP  - 1659
VL  - 66
DO  - 10.2298/ABS1404659M
UR  - conv_1138
ER  - 

@article{
author = "Milanović, Slobodan and Mihajlović, Ljubodrag and Karadžić, Dragan and Jankovsky, Libor and Aleksić, Predrag and Janković-Tomanić, Milena and Lazarević, Jelica",
year = "2014",
abstract = "Gypsy moths and powdery mildew play a significant role in oak decline processes. However, information is lacking on the effects on the gypsy moth of impaired tree vitality caused by defoliation or parasite infection. We assessed how pedunculate oak leaves collected from vigorous, declining, and infected trees influenced gypsy moth preference and performance (growth and nutritional indices). We found a negative effect of powdery mildew-infected leaves on gypsy moth performance, while declining trees had positive effects on gypsy moth performance and preference. All examined parameters of larvae fed declining oak leaves were higher than those of larvae fed vigorous oak leaves. Increased growth on declining oak leaves was caused by both higher consumption and more efficient food utilization. The results of this research could help us to better understand multitrophic interactions in complex communities such as oak forests.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effects of pedunculate oak tree vitality on gypsy moth preference and performance",
pages = "1672-1659",
number = "4",
volume = "66",
doi = "10.2298/ABS1404659M",
url = "conv_1138"
}

Milanović, S., Mihajlović, L., Karadžić, D., Jankovsky, L., Aleksić, P., Janković-Tomanić, M.,& Lazarević, J.. (2014). Effects of pedunculate oak tree vitality on gypsy moth preference and performance. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(4), 1659-1672.
https://doi.org/10.2298/ABS1404659M
conv_1138

Milanović S, Mihajlović L, Karadžić D, Jankovsky L, Aleksić P, Janković-Tomanić M, Lazarević J. Effects of pedunculate oak tree vitality on gypsy moth preference and performance. in Archives of Biological Sciences. 2014;66(4):1659-1672.
doi:10.2298/ABS1404659M
conv_1138 .

Milanović, Slobodan, Mihajlović, Ljubodrag, Karadžić, Dragan, Jankovsky, Libor, Aleksić, Predrag, Janković-Tomanić, Milena, Lazarević, Jelica, "Effects of pedunculate oak tree vitality on gypsy moth preference and performance" in Archives of Biological Sciences, 66, no. 4 (2014):1659-1672,
https://doi.org/10.2298/ABS1404659M .,
conv_1138 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Nenadović, Vera
AU  - Janković-Tomanić, Milena
AU  - Milanović, Slobodan
PY  - 2008
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/222
AB  - Periodic fluctuations in density impose different selection pressures on populations of outbreaking Lepidoptera due to changes in their nutritional environment. The maternal effects hypothesis of insect outbreak predicts the transmission of this nutritional "information" to subsequent generations and alterations in offspring life-history traits. To test for these time-delayed effects of the parental generation, we compared life-history traits and their variation and covariation among laboratory-reared gypsy moths hatched from egg masses collected from low- and medium-density populations. Decreased individual performance was recorded in offspring from the medium-density population, indicating reduced egg provisioning under crowding conditions. Genetic variance and covariance were also shown to be sensitive to density of the parental generation. In gypsy moths from the medium-density population, quantitative genetic analysis revealed significantly higher broad-sense heritabilities for development duration traits and demonstrated a trade-off between development duration and body size.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Genetic variation and correlations of life-history traits in gypsy moths ( lymantria dispar l.) From two populations in Serbia
EP  - 627
IS  - 4
SP  - 619
VL  - 60
DO  - 10.2298/ABS0804619L
UR  - conv_883
ER  - 

@article{
author = "Lazarević, Jelica and Nenadović, Vera and Janković-Tomanić, Milena and Milanović, Slobodan",
year = "2008",
abstract = "Periodic fluctuations in density impose different selection pressures on populations of outbreaking Lepidoptera due to changes in their nutritional environment. The maternal effects hypothesis of insect outbreak predicts the transmission of this nutritional "information" to subsequent generations and alterations in offspring life-history traits. To test for these time-delayed effects of the parental generation, we compared life-history traits and their variation and covariation among laboratory-reared gypsy moths hatched from egg masses collected from low- and medium-density populations. Decreased individual performance was recorded in offspring from the medium-density population, indicating reduced egg provisioning under crowding conditions. Genetic variance and covariance were also shown to be sensitive to density of the parental generation. In gypsy moths from the medium-density population, quantitative genetic analysis revealed significantly higher broad-sense heritabilities for development duration traits and demonstrated a trade-off between development duration and body size.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Genetic variation and correlations of life-history traits in gypsy moths ( lymantria dispar l.) From two populations in Serbia",
pages = "627-619",
number = "4",
volume = "60",
doi = "10.2298/ABS0804619L",
url = "conv_883"
}

Lazarević, J., Nenadović, V., Janković-Tomanić, M.,& Milanović, S.. (2008). Genetic variation and correlations of life-history traits in gypsy moths ( lymantria dispar l.) From two populations in Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(4), 619-627.
https://doi.org/10.2298/ABS0804619L
conv_883

Lazarević J, Nenadović V, Janković-Tomanić M, Milanović S. Genetic variation and correlations of life-history traits in gypsy moths ( lymantria dispar l.) From two populations in Serbia. in Archives of Biological Sciences. 2008;60(4):619-627.
doi:10.2298/ABS0804619L
conv_883 .

Lazarević, Jelica, Nenadović, Vera, Janković-Tomanić, Milena, Milanović, Slobodan, "Genetic variation and correlations of life-history traits in gypsy moths ( lymantria dispar l.) From two populations in Serbia" in Archives of Biological Sciences, 60, no. 4 (2008):619-627,
https://doi.org/10.2298/ABS0804619L .,
conv_883 .