The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).
TY - JOUR
AU - Marković, Marija
AU - Grbić, Mihailo
AU - Đukić, Matilda
PY - 2016
UR - https://omorika.sfb.bg.ac.rs/handle/123456789/724
AB - The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).
T2 - Brazilian Archives of Biology and Technology
T1 - Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm
VL - 59
DO - 10.1590/1678-4324-2016150320
UR - conv_1212
ER -
@article{
author = "Marković, Marija and Grbić, Mihailo and Đukić, Matilda",
year = "2016",
abstract = "The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).",
journal = "Brazilian Archives of Biology and Technology",
title = "Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm",
volume = "59",
doi = "10.1590/1678-4324-2016150320",
url = "conv_1212"
}
Marković, M., Grbić, M.,& Đukić, M.. (2016). Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm. in Brazilian Archives of Biology and Technology, 59.
https://doi.org/10.1590/1678-4324-2016150320
conv_1212
Marković M, Grbić M, Đukić M. Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm. in Brazilian Archives of Biology and Technology. 2016;59.
doi:10.1590/1678-4324-2016150320
conv_1212 .
Marković, Marija, Grbić, Mihailo, Đukić, Matilda, "Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm" in Brazilian Archives of Biology and Technology, 59 (2016),
https://doi.org/10.1590/1678-4324-2016150320 .,
conv_1212 .
