Primena kulture in vitro u razmnožavanju ugroženih taksona roda Dianthus L.

Abstract

U radu je dat prikaz istraživanja vezanih za in vitro razmnožavanje 30 ugroženih taksona roda Dianthus L. Ustanovljeni protokoli razmnožavanja su analizirani i sumirani, pri čemu su dati osnovni principi, metode i preporuke. Ustanovljeni protokoli se mogu koristiti za razmnožavanje ugroženih karanfila u cilju dobijanja materijala za sadnju na prirodno stanište. Takođe, dobijeni rezultati predstavljaju osnovu za ex situ konzervaciju ispitivanih taksona, pre svega njihovo kratkoročno ili dugoročno skladištenje (krioprezervacija).

The review of recent researches regarding the in vitro culture of 30 endangered Dianthus taxa is presented in this paper. Various in vitro protocols developed for selected rare and threatened Dianthus taxa are analysed in order to provide a useful synthesis of the data obtained with the main principles, techniques and recommendations for futher research and practice. The recapitulated data presented in this review can be used as a tool for the micropropagation of other endangered Dianthus taxa, enabling their propagation and obtaining a sufficient amount of plants for reintroduction. In addition, the obtained results represent the basis for ex situ conservation of the investigated taxa, especially for medium-term and long-term conservation (cryopreservation).