Uticaj tipa eksplanta na ožiljavanje i aklimatizaciju vrste Dianthus serotinus waldst. & Kit.

Abstract

U radu je ispitan uticaj koncentracije MS soli i tipa eksplanta na ožiljavanje i aklimatizaciju Dianthus serotinus u cilju optimizacije protokola za mikropropagaciju ove vrste. Pokazano je da tip eksplanta i koncentracija MS soli značajno utiču na ožiljavanje. Najviši procenat ožiljavanja (85-86,7%) dobijen je korišćenjem jednonodusnih reznica i terminalnih pupoljaka na podlozi sa 1/2MS koncentracijom soli i 0,5 mgL-1NAA. Međutim, prosečan broj korenova po eksplantu bio je veći na MS (15,3-18,6) nego na 1/2MS podlogama (11,8-13,4). Procenat aklimatizacije bio je najviši na mešavini treseta i peska u odnosu 4:1 (83,3-86,7%), a tip eksplanta iz kog se razvila ožiljena in vitro biljka nije imao uticaja na aklimatizaciju.

The effect of the concentration of MS salts and explant type on D. serotinus rooting and acclimatization was investigated in order to optimize a protocol for the micropropagation of this species. The obtained results showed that explant type as well as the concentration of MS salts had a significant effect on rooting, and the highest rooting rate (85-86,7%) was achieved when culturing singlenode cuttings and terminal buds on a half-strength MS medium supplemented with 0,5 mgL-1NAA. Nevertheless, mean number of roots per explant was higher on the MS media (15,3-18,6) than on the half-strength MS media (11,8-13,4). The best acclimatization rate was obtained in a 4:1 mixture of peat and sand (83,3-86,7%). The explant type from which in vitro plantlets developed had no effect on the acclimatization rate.