Razmnožavanje Dianthus deltoides L. kulturom izdanaka

Abstract

Ispitana je mogućnost unapređenja mikropropagacije vrste D. deltoides sa ciljem smanjenja broja vitrifikovanih i nekrotiranih izdanaka. Mikropropagacija je izvršena na medijumu sa MS mineralnim rastvorom, uz dodatak 3% saharoze, 0,8% agara, 50 mg·L-1 mio-inozitola, 0,025 mg·L-1 tiamina, 0,125 mg·L-1 nikotinske kiseline i 0,5 mg·L-1 glicina. U fazi multiplikacije izdanaka korišćen je 6-benzil-aminopurin (BAP) i α-naftil-sirćetna kiselina (NAA). Vitrifikacija je uspešno redukovana, a najbolji rezultati postignuti su na medijumu sa 1 mg·L-1 BAP i 0,1 mg·L-1 NAA. Ožiljavanje je uspešno sprovedeno na medijumu bez hormona, a procenat aklimatizacije iznosio je 92%.

The possibility of improving D. deltoides micropropagation was studied in the aim of reducing the number of vitrified and necrotic shoots. Micropropagation was performed on the medium with MS mineral solution, supplemented with 3% sucrose, 0.8% agar, 50 mg·L-1 mio-inositol, 0.025 mg·L-1 thiamin, 0.125 mg·L-1 nicotinic acid and 0.5 mg·L-1 glycine. The shoot multiplication was induced on 6-benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA). Vitrification was successfully reduced, and the best results were achieved on the medium with 1 mg·L-1 BAP and 0.1 mg·L-1 NAA. Rooting was successful on the medium without hormones, and the acclimatisation percentage accounted for 92%.