TY  - JOUR
AU  - Milenković, Ivan
AU  - Nowakowska, Justyna A.
AU  - Oszako, Tomasz
AU  - Mladenović, Katarina
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Karadžić, Dragan
PY  - 2014
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/578
AB  - The paper presents the results of the study performed with aims to determine the presence and diversity of Phytophthora species on maple trees in Serbia. Due to high aggressiveness and their multicyclic nature, presence of these pathogens is posing significant threat to forestry and biodiversity. In total, 29 samples of water, soil and tissues were taken from 10 different localities, and six different maple hosts were tested. After the isolation tests, 17 samples from five different maple hosts were positive for the presence of Phytophthora spp., and 31 isolates were obtained. After the detailed morphological and physiological classification, four distinct groups of isolates were separated. DNA was extracted from selected representative isolates and molecular identification with sequencing of ITS region was performed. Used ITS4 and ITS6 primers successfully amplified the genomic DNA of chosen isolates and morphological identification of obtained isolates was confirmed after the sequencing. Four different Phytophthora species were detected, including P. cactorum, P. gonapodyides, P. plurivora and P. lacustris. The most common isolated species was homothallic, and with very variable and semipapillate sporangia, P. plurivora with 22 obtained isolates. This is the first report of P. plurivora and P. gonapodyides on A. campestre, P. plurivora and P. lacustris on Acer heldreichii and first report of P. lacustris on A. pseudoplatanus and A. tataricum in Serbia.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Morphological and molecular identification of phytophthora species from maple trees in Serbia
EP  - 368
IS  - 2
SP  - 353
VL  - 46
DO  - 10.2298/GENSR1402353M
UR  - conv_1129
ER  - 

@article{
author = "Milenković, Ivan and Nowakowska, Justyna A. and Oszako, Tomasz and Mladenović, Katarina and Lučić, Aleksandar and Rakonjac, Ljubinko and Karadžić, Dragan",
year = "2014",
abstract = "The paper presents the results of the study performed with aims to determine the presence and diversity of Phytophthora species on maple trees in Serbia. Due to high aggressiveness and their multicyclic nature, presence of these pathogens is posing significant threat to forestry and biodiversity. In total, 29 samples of water, soil and tissues were taken from 10 different localities, and six different maple hosts were tested. After the isolation tests, 17 samples from five different maple hosts were positive for the presence of Phytophthora spp., and 31 isolates were obtained. After the detailed morphological and physiological classification, four distinct groups of isolates were separated. DNA was extracted from selected representative isolates and molecular identification with sequencing of ITS region was performed. Used ITS4 and ITS6 primers successfully amplified the genomic DNA of chosen isolates and morphological identification of obtained isolates was confirmed after the sequencing. Four different Phytophthora species were detected, including P. cactorum, P. gonapodyides, P. plurivora and P. lacustris. The most common isolated species was homothallic, and with very variable and semipapillate sporangia, P. plurivora with 22 obtained isolates. This is the first report of P. plurivora and P. gonapodyides on A. campestre, P. plurivora and P. lacustris on Acer heldreichii and first report of P. lacustris on A. pseudoplatanus and A. tataricum in Serbia.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Morphological and molecular identification of phytophthora species from maple trees in Serbia",
pages = "368-353",
number = "2",
volume = "46",
doi = "10.2298/GENSR1402353M",
url = "conv_1129"
}

Milenković, I., Nowakowska, J. A., Oszako, T., Mladenović, K., Lučić, A., Rakonjac, L.,& Karadžić, D.. (2014). Morphological and molecular identification of phytophthora species from maple trees in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 46(2), 353-368.
https://doi.org/10.2298/GENSR1402353M
conv_1129

Milenković I, Nowakowska JA, Oszako T, Mladenović K, Lučić A, Rakonjac L, Karadžić D. Morphological and molecular identification of phytophthora species from maple trees in Serbia. in Genetika-Belgrade. 2014;46(2):353-368.
doi:10.2298/GENSR1402353M
conv_1129 .

Milenković, Ivan, Nowakowska, Justyna A., Oszako, Tomasz, Mladenović, Katarina, Lučić, Aleksandar, Rakonjac, Ljubinko, Karadžić, Dragan, "Morphological and molecular identification of phytophthora species from maple trees in Serbia" in Genetika-Belgrade, 46, no. 2 (2014):353-368,
https://doi.org/10.2298/GENSR1402353M .,
conv_1129 .