TY  - JOUR
AU  - Jung, Thomas
AU  - Horta Jung, Marilia
AU  - Webber, Joan
AU  - Kageyama, Koji
AU  - Hieno, Ayaka
AU  - Masuya, Hayato
AU  - Uematsu, Seiji
AU  - Perez-Sierra, Ana
AU  - Harris, Anna R.
AU  - Forster, Jack
AU  - Rees, Helen
AU  - Scanu, Bruno
AU  - Patra, Sneha
AU  - Kudlacek, Tomas
AU  - Janousek, Josef
AU  - Corcobado, Tamara
AU  - Milenković, Ivan
AU  - Nagy, Zoltan
AU  - Csorba, Ildiko
AU  - Bakonyi, Jozsef
AU  - Brasier, Clive M.
PY  - 2021
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1217
AB  - As global plant trade expands, tree disease epidemics caused by pathogen introductions are increasing. Since ca 2000, the introduced oomycete Phytophthora ramorum has caused devastating epidemics in Europe and North America, spreading as four ancient clonal lineages, each of a single mating type, suggesting different geographical origins. We surveyed laurosilva forests for P. ramorum around Fansipan mountain on the Vietnam-China border and on Shikoku and Kyushu islands, southwest Japan. The surveys yielded 71 P. ramorum isolates which we assigned to eight new lineages, IC1 to IC5 from Vietnam and NP1 to NP3 from Japan, based on differences in colony characteristics, gene x environment responses and multigene phylogeny. Molecular phylogenetic trees and networks revealed the eight Asian lineages were dispersed across the topology of the introduced European and North American lineages. The deepest node within P. ramorum, the divergence of lineages NP1 and NP2, was estimated at 0.5 to 1.6 Myr. The Asian lineages were each of a single mating type, and at some locations, lineages of "opposite" mating type were present, suggesting opportunities for inter-lineage recombination. Based on the high level of phenotypic and phylogenetic diversity in the sample populations, the coalescence results and the absence of overt host symptoms, we conclude that P. ramorum comprises many anciently divergent lineages native to the laurosilva forests between eastern Indochina and Japan.
T2  - Journal of Fungi
T1  - The Destructive Tree Pathogen Phytophthora ramorum Originates from the Laurosilva Forests of East Asia
IS  - 3
VL  - 7
DO  - 10.3390/jof7030226
UR  - conv_1536
ER  - 

@article{
author = "Jung, Thomas and Horta Jung, Marilia and Webber, Joan and Kageyama, Koji and Hieno, Ayaka and Masuya, Hayato and Uematsu, Seiji and Perez-Sierra, Ana and Harris, Anna R. and Forster, Jack and Rees, Helen and Scanu, Bruno and Patra, Sneha and Kudlacek, Tomas and Janousek, Josef and Corcobado, Tamara and Milenković, Ivan and Nagy, Zoltan and Csorba, Ildiko and Bakonyi, Jozsef and Brasier, Clive M.",
year = "2021",
abstract = "As global plant trade expands, tree disease epidemics caused by pathogen introductions are increasing. Since ca 2000, the introduced oomycete Phytophthora ramorum has caused devastating epidemics in Europe and North America, spreading as four ancient clonal lineages, each of a single mating type, suggesting different geographical origins. We surveyed laurosilva forests for P. ramorum around Fansipan mountain on the Vietnam-China border and on Shikoku and Kyushu islands, southwest Japan. The surveys yielded 71 P. ramorum isolates which we assigned to eight new lineages, IC1 to IC5 from Vietnam and NP1 to NP3 from Japan, based on differences in colony characteristics, gene x environment responses and multigene phylogeny. Molecular phylogenetic trees and networks revealed the eight Asian lineages were dispersed across the topology of the introduced European and North American lineages. The deepest node within P. ramorum, the divergence of lineages NP1 and NP2, was estimated at 0.5 to 1.6 Myr. The Asian lineages were each of a single mating type, and at some locations, lineages of "opposite" mating type were present, suggesting opportunities for inter-lineage recombination. Based on the high level of phenotypic and phylogenetic diversity in the sample populations, the coalescence results and the absence of overt host symptoms, we conclude that P. ramorum comprises many anciently divergent lineages native to the laurosilva forests between eastern Indochina and Japan.",
journal = "Journal of Fungi",
title = "The Destructive Tree Pathogen Phytophthora ramorum Originates from the Laurosilva Forests of East Asia",
number = "3",
volume = "7",
doi = "10.3390/jof7030226",
url = "conv_1536"
}

Jung, T., Horta Jung, M., Webber, J., Kageyama, K., Hieno, A., Masuya, H., Uematsu, S., Perez-Sierra, A., Harris, A. R., Forster, J., Rees, H., Scanu, B., Patra, S., Kudlacek, T., Janousek, J., Corcobado, T., Milenković, I., Nagy, Z., Csorba, I., Bakonyi, J.,& Brasier, C. M.. (2021). The Destructive Tree Pathogen Phytophthora ramorum Originates from the Laurosilva Forests of East Asia. in Journal of Fungi, 7(3).
https://doi.org/10.3390/jof7030226
conv_1536

Jung T, Horta Jung M, Webber J, Kageyama K, Hieno A, Masuya H, Uematsu S, Perez-Sierra A, Harris AR, Forster J, Rees H, Scanu B, Patra S, Kudlacek T, Janousek J, Corcobado T, Milenković I, Nagy Z, Csorba I, Bakonyi J, Brasier CM. The Destructive Tree Pathogen Phytophthora ramorum Originates from the Laurosilva Forests of East Asia. in Journal of Fungi. 2021;7(3).
doi:10.3390/jof7030226
conv_1536 .

Jung, Thomas, Horta Jung, Marilia, Webber, Joan, Kageyama, Koji, Hieno, Ayaka, Masuya, Hayato, Uematsu, Seiji, Perez-Sierra, Ana, Harris, Anna R., Forster, Jack, Rees, Helen, Scanu, Bruno, Patra, Sneha, Kudlacek, Tomas, Janousek, Josef, Corcobado, Tamara, Milenković, Ivan, Nagy, Zoltan, Csorba, Ildiko, Bakonyi, Jozsef, Brasier, Clive M., "The Destructive Tree Pathogen Phytophthora ramorum Originates from the Laurosilva Forests of East Asia" in Journal of Fungi, 7, no. 3 (2021),
https://doi.org/10.3390/jof7030226 .,
conv_1536 .

TY  - JOUR
AU  - Jung, Thomas
AU  - Scanu, Bruno
AU  - Brasier, Clive M.
AU  - Webber, Joan
AU  - Milenković, Ivan
AU  - Corcobado, Tamara
AU  - Tomsovsky, Michal
AU  - Panek, Matej
AU  - Bakonyi, Jozsef
AU  - Maia, Cristiana
AU  - Bacova, Aneta
AU  - Raco, Milica
AU  - Rees, Helen
AU  - Perez-Sierra, Ana
AU  - Horta Jung, Marilia
PY  - 2020
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1137
AB  - In 2016 and 2017, surveys of Phytophthora diversity were performed in 25 natural and semi-natural forest stands and 16 rivers in temperate and subtropical montane and tropical lowland regions of Vietnam. Using baiting assays from soil samples and rivers and direct isolations from naturally fallen leaves, 13 described species, five informally designated taxa and 21 previously unknown taxa of Phytophthora were isolated from 58 of the 91 soil samples (63.7%) taken from the rhizosphere of 52 of the 64 woody plant species sampled (81.3%) in 20 forest stands (83.7%), and from all rivers: P. capensis, P. citricola VII, VIII, IX, X and XI, P. sp. botryosa-like 2, P. sp. meadii-like 1 and 2, P. sp. tropicalis-like 2 and P. sp. multivesiculata-like 1 from Phytophthora major phylogenetic Clade 2; P. castaneae and P. heveae from Clade 5; P. chlamydospora, P. gregata, P. sp. bitahaiensis-like and P. sp. sylvatica-like 1, 2 and 3 from Clade 6; P. cinnamomi (Pc), P. parvispora, P. attenuata, P. sp. attenuata-like 1, 2 and 3 and P. xheterohybrida from Clade 7; P. drechsleri, P. pseudocryptogea, P. ramorum (Pr) and P. sp. kelmania from Clade 8, P. macrochlamydospora, P. sp. xinsolita-like, P. sp. xkunnunara-like, P. sp. xvirginiana-like s.l. and three new taxa, P. sp. quininea-like, P. sp. xGrenada 3-like and P. sp. xPeru 4-like, from Clade 9; and P. sp. gallica-like 1 and 2 from Clade 10. The Al and A2 mating types of both Pc and Pr co-occurred. The A2 mating type of Pc was associated with severe dieback of montane forests in northern Vietnam. Most other Phytophthora species, including Pr, were not associated with obvious disease symptoms. It is concluded that (1) Vietnam is within the center of origin of most Phytophthora taxa found including Pc and Pr, and (2) Phytophthora clades 2, 5, 6, 7, 8, 9, and 10 are native to Indochina.
T2  - Forests
T1  - A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum
IS  - 1
VL  - 11
DO  - 10.3390/f11010093
UR  - conv_1479
ER  - 

@article{
author = "Jung, Thomas and Scanu, Bruno and Brasier, Clive M. and Webber, Joan and Milenković, Ivan and Corcobado, Tamara and Tomsovsky, Michal and Panek, Matej and Bakonyi, Jozsef and Maia, Cristiana and Bacova, Aneta and Raco, Milica and Rees, Helen and Perez-Sierra, Ana and Horta Jung, Marilia",
year = "2020",
abstract = "In 2016 and 2017, surveys of Phytophthora diversity were performed in 25 natural and semi-natural forest stands and 16 rivers in temperate and subtropical montane and tropical lowland regions of Vietnam. Using baiting assays from soil samples and rivers and direct isolations from naturally fallen leaves, 13 described species, five informally designated taxa and 21 previously unknown taxa of Phytophthora were isolated from 58 of the 91 soil samples (63.7%) taken from the rhizosphere of 52 of the 64 woody plant species sampled (81.3%) in 20 forest stands (83.7%), and from all rivers: P. capensis, P. citricola VII, VIII, IX, X and XI, P. sp. botryosa-like 2, P. sp. meadii-like 1 and 2, P. sp. tropicalis-like 2 and P. sp. multivesiculata-like 1 from Phytophthora major phylogenetic Clade 2; P. castaneae and P. heveae from Clade 5; P. chlamydospora, P. gregata, P. sp. bitahaiensis-like and P. sp. sylvatica-like 1, 2 and 3 from Clade 6; P. cinnamomi (Pc), P. parvispora, P. attenuata, P. sp. attenuata-like 1, 2 and 3 and P. xheterohybrida from Clade 7; P. drechsleri, P. pseudocryptogea, P. ramorum (Pr) and P. sp. kelmania from Clade 8, P. macrochlamydospora, P. sp. xinsolita-like, P. sp. xkunnunara-like, P. sp. xvirginiana-like s.l. and three new taxa, P. sp. quininea-like, P. sp. xGrenada 3-like and P. sp. xPeru 4-like, from Clade 9; and P. sp. gallica-like 1 and 2 from Clade 10. The Al and A2 mating types of both Pc and Pr co-occurred. The A2 mating type of Pc was associated with severe dieback of montane forests in northern Vietnam. Most other Phytophthora species, including Pr, were not associated with obvious disease symptoms. It is concluded that (1) Vietnam is within the center of origin of most Phytophthora taxa found including Pc and Pr, and (2) Phytophthora clades 2, 5, 6, 7, 8, 9, and 10 are native to Indochina.",
journal = "Forests",
title = "A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum",
number = "1",
volume = "11",
doi = "10.3390/f11010093",
url = "conv_1479"
}

Jung, T., Scanu, B., Brasier, C. M., Webber, J., Milenković, I., Corcobado, T., Tomsovsky, M., Panek, M., Bakonyi, J., Maia, C., Bacova, A., Raco, M., Rees, H., Perez-Sierra, A.,& Horta Jung, M.. (2020). A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum. in Forests, 11(1).
https://doi.org/10.3390/f11010093
conv_1479

Jung T, Scanu B, Brasier CM, Webber J, Milenković I, Corcobado T, Tomsovsky M, Panek M, Bakonyi J, Maia C, Bacova A, Raco M, Rees H, Perez-Sierra A, Horta Jung M. A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum. in Forests. 2020;11(1).
doi:10.3390/f11010093
conv_1479 .

Jung, Thomas, Scanu, Bruno, Brasier, Clive M., Webber, Joan, Milenković, Ivan, Corcobado, Tamara, Tomsovsky, Michal, Panek, Matej, Bakonyi, Jozsef, Maia, Cristiana, Bacova, Aneta, Raco, Milica, Rees, Helen, Perez-Sierra, Ana, Horta Jung, Marilia, "A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum" in Forests, 11, no. 1 (2020),
https://doi.org/10.3390/f11010093 .,
conv_1479 .

TY  - JOUR
AU  - Milenković, Ivan
AU  - Keča, Nenad
AU  - Karadžić, Dragan
AU  - Radulović, Zlatan
AU  - Nowakowska, Justyna A.
AU  - Oszako, Tomasz
AU  - Sikora, Katarzyna
AU  - Corcobado, Tamara
AU  - Jung, Thomas
PY  - 2018
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/916
AB  - During a survey in three declining and three healthy poplar plantations in Serbia, six different Phytophthora species were obtained. Phytophthora plurivora was the most common, followed by P. pini, P. polonica, P. lacustris, P. cactorum, and P. gonapodyides. Pathogenicity of all isolated species to four-month and one-year-old cuttings of Populus hybrid clones I-214 and Pannonia, respectively, was tested using both a soil infestation and stem inoculation test. Isolates of P. polonica, P. x cambivora, P. cryptogea, and P. x serendipita from other host plants were included as a comparison. In the soil infestation test, the most aggressive species to clone I-214 were P. plurivora, P. x serendipita, and P. pini. On clone Pannonia, P. gonapodyides and P. pini were the most aggressive, both causing 100% mortality, followed by P. cactorum, P. x cambivora, and P. polonica. In the underbark inoculation test, the susceptibility of both poplar clones to the different Phytophthora species was largely similar, as in the soil infestation test, with the exception of P. polonica, which proved to be only weakly pathogenic to poplar bark. The most aggressive species to clone I-214 was P. pini, while on clone Pannonia, the longest lesions and highest disease incidence were caused by P. gonapodyides. Phytophthora cactorum and P. plurivora were pathogenic to both clones, whereas P. x cambivora showed only weak pathogenicity. The implications of these findings and possible pathways of dispersion of the pathogens are discussed.
T2  - Forests
T1  - Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia
IS  - 6
VL  - 9
DO  - 10.3390/f9060330
UR  - conv_1351
ER  - 

@article{
author = "Milenković, Ivan and Keča, Nenad and Karadžić, Dragan and Radulović, Zlatan and Nowakowska, Justyna A. and Oszako, Tomasz and Sikora, Katarzyna and Corcobado, Tamara and Jung, Thomas",
year = "2018",
abstract = "During a survey in three declining and three healthy poplar plantations in Serbia, six different Phytophthora species were obtained. Phytophthora plurivora was the most common, followed by P. pini, P. polonica, P. lacustris, P. cactorum, and P. gonapodyides. Pathogenicity of all isolated species to four-month and one-year-old cuttings of Populus hybrid clones I-214 and Pannonia, respectively, was tested using both a soil infestation and stem inoculation test. Isolates of P. polonica, P. x cambivora, P. cryptogea, and P. x serendipita from other host plants were included as a comparison. In the soil infestation test, the most aggressive species to clone I-214 were P. plurivora, P. x serendipita, and P. pini. On clone Pannonia, P. gonapodyides and P. pini were the most aggressive, both causing 100% mortality, followed by P. cactorum, P. x cambivora, and P. polonica. In the underbark inoculation test, the susceptibility of both poplar clones to the different Phytophthora species was largely similar, as in the soil infestation test, with the exception of P. polonica, which proved to be only weakly pathogenic to poplar bark. The most aggressive species to clone I-214 was P. pini, while on clone Pannonia, the longest lesions and highest disease incidence were caused by P. gonapodyides. Phytophthora cactorum and P. plurivora were pathogenic to both clones, whereas P. x cambivora showed only weak pathogenicity. The implications of these findings and possible pathways of dispersion of the pathogens are discussed.",
journal = "Forests",
title = "Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia",
number = "6",
volume = "9",
doi = "10.3390/f9060330",
url = "conv_1351"
}

Milenković, I., Keča, N., Karadžić, D., Radulović, Z., Nowakowska, J. A., Oszako, T., Sikora, K., Corcobado, T.,& Jung, T.. (2018). Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia. in Forests, 9(6).
https://doi.org/10.3390/f9060330
conv_1351

Milenković I, Keča N, Karadžić D, Radulović Z, Nowakowska JA, Oszako T, Sikora K, Corcobado T, Jung T. Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia. in Forests. 2018;9(6).
doi:10.3390/f9060330
conv_1351 .

Milenković, Ivan, Keča, Nenad, Karadžić, Dragan, Radulović, Zlatan, Nowakowska, Justyna A., Oszako, Tomasz, Sikora, Katarzyna, Corcobado, Tamara, Jung, Thomas, "Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia" in Forests, 9, no. 6 (2018),
https://doi.org/10.3390/f9060330 .,
conv_1351 .

TY  - JOUR
AU  - Milenković, Ivan
AU  - Keča, Nenad
AU  - Karadžić, Dragan
AU  - Radulović, Zlatan
AU  - Tomsovsky, M.
AU  - Jung, T.
PY  - 2018
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/970
AB  - Cherry laurel (Prunus laurocerasus) is a native plant species in Serbian forests, but is also widely used for ornamental plantings. Following two extremely wet summers in 2014 and 2015, in spring and summer of 2016 and 2017, numerous cherry laurel plants with symptoms indicative for Phytophthora diseases, like wilting and chlorosis of leaves, dieback and bleeding bark necroses, were recorded in a park in Belgrade and in two ornamental nurseries in central Serbia. From necrotic bark samples and rhizosphere soil, self-sterile Phytophthora isolates with woolly colonies were obtained. Due to the production of ellipsoid and elongated, non-papillate sporangia in water and of ornamented oogonia with two-celled antheridia in mating tests with tester strains of both Phytophthora x cambivora and P.cryptogea, these isolates were identified as P.xcambivora which was confirmed by ITS sequence analysis. Pathogenicity of P.xcambivora from cherry laurel (PCCL) was tested by inoculating one-year-old seedlings of cherry laurel under the bark. P.xcambivora from European beech (PCB), and isolates of P.cactorum (CAC), P.cryptogea (CRY), P.plurivora (PLU) and P.xserendipita (SER) were included as comparison. Three and a half months after inoculation, nine of the twelve plants in PCB, three in PCCL and CAC and two in PLU declined with longitudinal necroses and chlorosis, wilting and premature shedding of leaves. These results demonstrate the ability of P.xcambivora to infect and cause decline of cherry laurel plants. The particularly high aggressiveness of the P.xcambivora isolate from beech shows that this pathogen poses a serious risk to cherry laurel in the rare natural communities of cherry laurel and beech in Serbia.
T2  - Forest Pathology
T1  - Occurrence and pathogenicity of Phytophthora x cambivora on Prunus laurocerasus in Serbia
IS  - 4
VL  - 48
DO  - 10.1111/efp.12436
UR  - conv_1361
ER  - 

@article{
author = "Milenković, Ivan and Keča, Nenad and Karadžić, Dragan and Radulović, Zlatan and Tomsovsky, M. and Jung, T.",
year = "2018",
abstract = "Cherry laurel (Prunus laurocerasus) is a native plant species in Serbian forests, but is also widely used for ornamental plantings. Following two extremely wet summers in 2014 and 2015, in spring and summer of 2016 and 2017, numerous cherry laurel plants with symptoms indicative for Phytophthora diseases, like wilting and chlorosis of leaves, dieback and bleeding bark necroses, were recorded in a park in Belgrade and in two ornamental nurseries in central Serbia. From necrotic bark samples and rhizosphere soil, self-sterile Phytophthora isolates with woolly colonies were obtained. Due to the production of ellipsoid and elongated, non-papillate sporangia in water and of ornamented oogonia with two-celled antheridia in mating tests with tester strains of both Phytophthora x cambivora and P.cryptogea, these isolates were identified as P.xcambivora which was confirmed by ITS sequence analysis. Pathogenicity of P.xcambivora from cherry laurel (PCCL) was tested by inoculating one-year-old seedlings of cherry laurel under the bark. P.xcambivora from European beech (PCB), and isolates of P.cactorum (CAC), P.cryptogea (CRY), P.plurivora (PLU) and P.xserendipita (SER) were included as comparison. Three and a half months after inoculation, nine of the twelve plants in PCB, three in PCCL and CAC and two in PLU declined with longitudinal necroses and chlorosis, wilting and premature shedding of leaves. These results demonstrate the ability of P.xcambivora to infect and cause decline of cherry laurel plants. The particularly high aggressiveness of the P.xcambivora isolate from beech shows that this pathogen poses a serious risk to cherry laurel in the rare natural communities of cherry laurel and beech in Serbia.",
journal = "Forest Pathology",
title = "Occurrence and pathogenicity of Phytophthora x cambivora on Prunus laurocerasus in Serbia",
number = "4",
volume = "48",
doi = "10.1111/efp.12436",
url = "conv_1361"
}

Milenković, I., Keča, N., Karadžić, D., Radulović, Z., Tomsovsky, M.,& Jung, T.. (2018). Occurrence and pathogenicity of Phytophthora x cambivora on Prunus laurocerasus in Serbia. in Forest Pathology, 48(4).
https://doi.org/10.1111/efp.12436
conv_1361

Milenković I, Keča N, Karadžić D, Radulović Z, Tomsovsky M, Jung T. Occurrence and pathogenicity of Phytophthora x cambivora on Prunus laurocerasus in Serbia. in Forest Pathology. 2018;48(4).
doi:10.1111/efp.12436
conv_1361 .

Milenković, Ivan, Keča, Nenad, Karadžić, Dragan, Radulović, Zlatan, Tomsovsky, M., Jung, T., "Occurrence and pathogenicity of Phytophthora x cambivora on Prunus laurocerasus in Serbia" in Forest Pathology, 48, no. 4 (2018),
https://doi.org/10.1111/efp.12436 .,
conv_1361 .