TY  - JOUR
AU  - Caballol, Maria
AU  - Redondo, Miguel A.
AU  - Catalan, Nuria
AU  - Corcobado, Tamara
AU  - Jung, Thomas
AU  - Marcais, Benoit
AU  - Milenković, Ivan
AU  - Nemesio-Gorriz, Miguel
AU  - Stenlid, Jan
AU  - Oliva, Jonas
PY  - 2024
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1517
AB  - Climate shapes the distribution of plant-associated microbes such as mycorrhizal and endophytic fungi. However, the role of climate in plant pathogen community assembly is less understood. Here, we explored the role of climate in the assembly of Phytophthora communities at  gt 250 sites along a latitudinal gradient from Spain to northern Sweden and an altitudinal gradient from the Spanish Pyrenees to lowland areas. Communities were detected by ITS sequencing of river filtrates. Mediation analysis supported the role of climate in the biogeography of Phytophthora and ruled out other environmental factors such as geography or tree diversity. Comparisons of functional and species diversity showed that environmental filtering dominated over competitive exclusion in Europe. Temperature and precipitation acted as environmental filters at different extremes of the gradients. In northern regions, winter temperatures acted as an environmental filter on Phytophthora community assembly, selecting species adapted to survive low minimum temperatures. In southern latitudes, a hot dry climate was the main environmental filter, resulting in communities dominated by drought-tolerant Phytophthora species with thick oospore walls, a high optimum temperature for growth, and a high maximum temperature limit for growth. By taking a community ecology approach, we show that the establishment of Phytophthora plant pathogens in Europe is mainly restricted by cold temperatures.
T2  - ISME Journal
T1  - Climate acts as an environmental filter to plant pathogens
IS  - 1
VL  - 18
DO  - 10.1093/ismejo/wrae010
UR  - conv_1787
ER  - 

@article{
author = "Caballol, Maria and Redondo, Miguel A. and Catalan, Nuria and Corcobado, Tamara and Jung, Thomas and Marcais, Benoit and Milenković, Ivan and Nemesio-Gorriz, Miguel and Stenlid, Jan and Oliva, Jonas",
year = "2024",
abstract = "Climate shapes the distribution of plant-associated microbes such as mycorrhizal and endophytic fungi. However, the role of climate in plant pathogen community assembly is less understood. Here, we explored the role of climate in the assembly of Phytophthora communities at  gt 250 sites along a latitudinal gradient from Spain to northern Sweden and an altitudinal gradient from the Spanish Pyrenees to lowland areas. Communities were detected by ITS sequencing of river filtrates. Mediation analysis supported the role of climate in the biogeography of Phytophthora and ruled out other environmental factors such as geography or tree diversity. Comparisons of functional and species diversity showed that environmental filtering dominated over competitive exclusion in Europe. Temperature and precipitation acted as environmental filters at different extremes of the gradients. In northern regions, winter temperatures acted as an environmental filter on Phytophthora community assembly, selecting species adapted to survive low minimum temperatures. In southern latitudes, a hot dry climate was the main environmental filter, resulting in communities dominated by drought-tolerant Phytophthora species with thick oospore walls, a high optimum temperature for growth, and a high maximum temperature limit for growth. By taking a community ecology approach, we show that the establishment of Phytophthora plant pathogens in Europe is mainly restricted by cold temperatures.",
journal = "ISME Journal",
title = "Climate acts as an environmental filter to plant pathogens",
number = "1",
volume = "18",
doi = "10.1093/ismejo/wrae010",
url = "conv_1787"
}

Caballol, M., Redondo, M. A., Catalan, N., Corcobado, T., Jung, T., Marcais, B., Milenković, I., Nemesio-Gorriz, M., Stenlid, J.,& Oliva, J.. (2024). Climate acts as an environmental filter to plant pathogens. in ISME Journal, 18(1).
https://doi.org/10.1093/ismejo/wrae010
conv_1787

Caballol M, Redondo MA, Catalan N, Corcobado T, Jung T, Marcais B, Milenković I, Nemesio-Gorriz M, Stenlid J, Oliva J. Climate acts as an environmental filter to plant pathogens. in ISME Journal. 2024;18(1).
doi:10.1093/ismejo/wrae010
conv_1787 .

Caballol, Maria, Redondo, Miguel A., Catalan, Nuria, Corcobado, Tamara, Jung, Thomas, Marcais, Benoit, Milenković, Ivan, Nemesio-Gorriz, Miguel, Stenlid, Jan, Oliva, Jonas, "Climate acts as an environmental filter to plant pathogens" in ISME Journal, 18, no. 1 (2024),
https://doi.org/10.1093/ismejo/wrae010 .,
conv_1787 .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Milenković, Ivan
AU  - Lazarević, Jelica
AU  - Todosijević, Marina M.
AU  - Ljujić, Jovana P.
AU  - Mitić, Zorica S.
AU  - Nikolić, Biljana M.
AU  - Marin, Petar D.
AU  - Tesević, Vele V.
PY  - 2024
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1485
AB  - Composition of Calocedrus decurrens and Cupressus arizonica essential oils (CDEO and CAEO, respectively), their antifeedant activity on spongy moth ( Lymantria dispar ) larvae, as well as their antimicrobial activity on three Phytophthora species were investigated. In leaves of these two conifers from the Cupressaceae family, monoterpenes strongly dominated (90.5 and 85.0%, respectively), but their terpene profiles were different. The dominant compound of CDEO was delta -3-carene (49.5%), followed by myrcene (9.4%), terpinolene (8.6%) and alpha -pinene (7.0%). In CAEO, the most dominant compounds were limonene (23.3%) and alpha -pinene (20.5%), followed by umbellulone (12.0%) and terpinen-4-ol (9.5%). CDEO showed a strong antifeedant activity, while leaf discs treated with CAEO had phagostimulatory effect on spongy moth larvae. CAEO had no significant effect on relative consumption and larval growth rate, while CDEO reduced consumption and stimulated growth at the lowest EO concentration (0.05%). Both EOs significantly affected the growth of colonies of the Phytophthora species tested. At concentrations of 0.5% and 1%, a 100% inhibition was observed in almost all experimental groups, with the exception of P. plurivora on 0.5% CDEO. At a concentration of 0.1% inhibition rate varied from 15% in P. x cambivora treated with CDEO to 90% in P. quercina treated with CAEO. These findings indicate that these two EOs could be used to control tree pests.
T2  - Industrial Crops and Products
T1  - Biological activity of essential oils of Calocedrus decurrens and Cupressus arizonica on Lymantria dispar larvae and Phytophthora root pathogens
VL  - 215
DO  - 10.1016/j.indcrop.2024.118602
UR  - conv_1788
ER  - 

@article{
author = "Milanović, Slobodan and Milenković, Ivan and Lazarević, Jelica and Todosijević, Marina M. and Ljujić, Jovana P. and Mitić, Zorica S. and Nikolić, Biljana M. and Marin, Petar D. and Tesević, Vele V.",
year = "2024",
abstract = "Composition of Calocedrus decurrens and Cupressus arizonica essential oils (CDEO and CAEO, respectively), their antifeedant activity on spongy moth ( Lymantria dispar ) larvae, as well as their antimicrobial activity on three Phytophthora species were investigated. In leaves of these two conifers from the Cupressaceae family, monoterpenes strongly dominated (90.5 and 85.0%, respectively), but their terpene profiles were different. The dominant compound of CDEO was delta -3-carene (49.5%), followed by myrcene (9.4%), terpinolene (8.6%) and alpha -pinene (7.0%). In CAEO, the most dominant compounds were limonene (23.3%) and alpha -pinene (20.5%), followed by umbellulone (12.0%) and terpinen-4-ol (9.5%). CDEO showed a strong antifeedant activity, while leaf discs treated with CAEO had phagostimulatory effect on spongy moth larvae. CAEO had no significant effect on relative consumption and larval growth rate, while CDEO reduced consumption and stimulated growth at the lowest EO concentration (0.05%). Both EOs significantly affected the growth of colonies of the Phytophthora species tested. At concentrations of 0.5% and 1%, a 100% inhibition was observed in almost all experimental groups, with the exception of P. plurivora on 0.5% CDEO. At a concentration of 0.1% inhibition rate varied from 15% in P. x cambivora treated with CDEO to 90% in P. quercina treated with CAEO. These findings indicate that these two EOs could be used to control tree pests.",
journal = "Industrial Crops and Products",
title = "Biological activity of essential oils of Calocedrus decurrens and Cupressus arizonica on Lymantria dispar larvae and Phytophthora root pathogens",
volume = "215",
doi = "10.1016/j.indcrop.2024.118602",
url = "conv_1788"
}

Milanović, S., Milenković, I., Lazarević, J., Todosijević, M. M., Ljujić, J. P., Mitić, Z. S., Nikolić, B. M., Marin, P. D.,& Tesević, V. V.. (2024). Biological activity of essential oils of Calocedrus decurrens and Cupressus arizonica on Lymantria dispar larvae and Phytophthora root pathogens. in Industrial Crops and Products, 215.
https://doi.org/10.1016/j.indcrop.2024.118602
conv_1788

Milanović S, Milenković I, Lazarević J, Todosijević MM, Ljujić JP, Mitić ZS, Nikolić BM, Marin PD, Tesević VV. Biological activity of essential oils of Calocedrus decurrens and Cupressus arizonica on Lymantria dispar larvae and Phytophthora root pathogens. in Industrial Crops and Products. 2024;215.
doi:10.1016/j.indcrop.2024.118602
conv_1788 .

Milanović, Slobodan, Milenković, Ivan, Lazarević, Jelica, Todosijević, Marina M., Ljujić, Jovana P., Mitić, Zorica S., Nikolić, Biljana M., Marin, Petar D., Tesević, Vele V., "Biological activity of essential oils of Calocedrus decurrens and Cupressus arizonica on Lymantria dispar larvae and Phytophthora root pathogens" in Industrial Crops and Products, 215 (2024),
https://doi.org/10.1016/j.indcrop.2024.118602 .,
conv_1788 .

TY  - JOUR
AU  - Corcobado, Tamara
AU  - Cech, Thomas L.
AU  - Daxer, Andreas
AU  - Datkova, Henrieta
AU  - Janousek, Josef
AU  - Patra, Sneha
AU  - Jahn, Daniella
AU  - Huettler, Christine
AU  - Milenković, Ivan
AU  - Tomsovsky, Michal
AU  - Horta Jung, Marilia
AU  - Jung, Thomas
PY  - 2023
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1441
AB  - Waterways are ideal pathways for Phytophthora dispersal and potential introduction to terrestrial ecosystems. While many Phytophthora species from phylogenetic clades 6, 9 and 10 are predominant oomycetes in watercourses due to their adaptation to a lifestyle as saprotrophs and opportunistic pathogens of riparian plants, species from clades 2, 7 and 8 are predominantly soil- or airborne using aquatic habitats as temporal niches for spreading and invading terrestrial sites along the watercourses. In contrast to forest ecosystems, knowledge of Phytophthora diversity in watercourses in Central Europe is limited. Between 2014 and 2019 extensive surveys of streams and rivers were undertaken across Austria, in South Moravia, Czech Republic and Zilina province, Slovakia to unveil the diversity and distribution of Phytophthora and related oomycetes. In addition, in Austria riparian forests of black alder (Alnus glutinosa) and grey alder (A. incana) in lowlands and in the Alps were examined. A variety of Phytophthora species from clades 2, 6, 7, 8, 9 and 10 were isolated, with clade 6 species showing the widest distribution and abundance. Furthermore, interspecific clade 6 hybrids and other oomycetes such as Halophytophthora fluviatilis and undescribed Nothophytophthora spp. were also obtained. In riparian alders, symptoms of Phytophthora infections were associated with species from the P.xalni complex and P. plurivora. Phytophthora plurivora was most common in alder stands whereas P. uniformis was the oomycete species occurring at the highest altitude in alpine riparian areas.
T2  - Mycological Progress
T1  - Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe
IS  - 7
VL  - 22
DO  - 10.1007/s11557-023-01898-1
UR  - conv_1732
ER  - 

@article{
author = "Corcobado, Tamara and Cech, Thomas L. and Daxer, Andreas and Datkova, Henrieta and Janousek, Josef and Patra, Sneha and Jahn, Daniella and Huettler, Christine and Milenković, Ivan and Tomsovsky, Michal and Horta Jung, Marilia and Jung, Thomas",
year = "2023",
abstract = "Waterways are ideal pathways for Phytophthora dispersal and potential introduction to terrestrial ecosystems. While many Phytophthora species from phylogenetic clades 6, 9 and 10 are predominant oomycetes in watercourses due to their adaptation to a lifestyle as saprotrophs and opportunistic pathogens of riparian plants, species from clades 2, 7 and 8 are predominantly soil- or airborne using aquatic habitats as temporal niches for spreading and invading terrestrial sites along the watercourses. In contrast to forest ecosystems, knowledge of Phytophthora diversity in watercourses in Central Europe is limited. Between 2014 and 2019 extensive surveys of streams and rivers were undertaken across Austria, in South Moravia, Czech Republic and Zilina province, Slovakia to unveil the diversity and distribution of Phytophthora and related oomycetes. In addition, in Austria riparian forests of black alder (Alnus glutinosa) and grey alder (A. incana) in lowlands and in the Alps were examined. A variety of Phytophthora species from clades 2, 6, 7, 8, 9 and 10 were isolated, with clade 6 species showing the widest distribution and abundance. Furthermore, interspecific clade 6 hybrids and other oomycetes such as Halophytophthora fluviatilis and undescribed Nothophytophthora spp. were also obtained. In riparian alders, symptoms of Phytophthora infections were associated with species from the P.xalni complex and P. plurivora. Phytophthora plurivora was most common in alder stands whereas P. uniformis was the oomycete species occurring at the highest altitude in alpine riparian areas.",
journal = "Mycological Progress",
title = "Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe",
number = "7",
volume = "22",
doi = "10.1007/s11557-023-01898-1",
url = "conv_1732"
}

Corcobado, T., Cech, T. L., Daxer, A., Datkova, H., Janousek, J., Patra, S., Jahn, D., Huettler, C., Milenković, I., Tomsovsky, M., Horta Jung, M.,& Jung, T.. (2023). Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe. in Mycological Progress, 22(7).
https://doi.org/10.1007/s11557-023-01898-1
conv_1732

Corcobado T, Cech TL, Daxer A, Datkova H, Janousek J, Patra S, Jahn D, Huettler C, Milenković I, Tomsovsky M, Horta Jung M, Jung T. Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe. in Mycological Progress. 2023;22(7).
doi:10.1007/s11557-023-01898-1
conv_1732 .

Corcobado, Tamara, Cech, Thomas L., Daxer, Andreas, Datkova, Henrieta, Janousek, Josef, Patra, Sneha, Jahn, Daniella, Huettler, Christine, Milenković, Ivan, Tomsovsky, Michal, Horta Jung, Marilia, Jung, Thomas, "Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe" in Mycological Progress, 22, no. 7 (2023),
https://doi.org/10.1007/s11557-023-01898-1 .,
conv_1732 .

TY  - JOUR
AU  - Maia, C.
AU  - Horta Jung, Marilia
AU  - Carella, G.
AU  - Milenković, Ivan
AU  - Janousek, J.
AU  - Tomsovsky, M.
AU  - Mosca, S.
AU  - Schena, L.
AU  - Cravador, A.
AU  - Moricca, S.
AU  - Jung, T.
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1374
AB  - During an oomycete survey in December 2015, 10 previously unknown Halophytophthora taxa were isolated from marine and brackish water of tidal ponds and channels in saltmarshes, lagoon ecosystems and river estuaries at seven sites along the Algarve coast in the South of Portugal. Phylogenetic analyses of LSU and ITS datasets, comprising all described Halophytophthora species, the 10 new Halophytophthora taxa and all relevant and distinctive sequences available from GenBank, provided an updated phylogeny of the genus Halophytophthora s.str. showing for the first time a structure of 10 clades designated as Clades 1-10. Nine of the 10 new Halophytophthora taxa resided in Clade 6 together with H. polymorphica and H. vesicula. Based on differences in morphology and temperature-growth relations and a multigene (LSU, ITS, Btub, hsp90, rpl10, tigA, cox1, nadh1, rps10) phylo-geny, eight new Halophytophthora taxa from Portugal are described here as H. brevisporangia, H. cele-ris, H. frigida, H. lateralis, H. lusitanica, H. macrosporangia, H. sinuata and H. thermoambigua. Three species, H. frigida, H. macrosporangia and H. sinuata, have a homothallic breeding system while the remaining five species are sterile. Pathogenicity and litter decomposition tests are underway to clarify their pathological and ecological role in the marine and brackish-water ecosystems. More oomycete surveys in yet undersurveyed regions of the world and population genetic or phylogenomic analyses of global populations are needed to clarify the origin of the new Halophytophthora species.
T2  - Persoonia
T1  - Eight new Halophytophthora species from marine and brackish-water ecosystems in Portugal and an updated phylogeny for the genus
EP  - 90
SP  - 54
VL  - 48
DO  - 10.3767/persoonia.2022.48.02
UR  - conv_1668
ER  - 

@article{
author = "Maia, C. and Horta Jung, Marilia and Carella, G. and Milenković, Ivan and Janousek, J. and Tomsovsky, M. and Mosca, S. and Schena, L. and Cravador, A. and Moricca, S. and Jung, T.",
year = "2022",
abstract = "During an oomycete survey in December 2015, 10 previously unknown Halophytophthora taxa were isolated from marine and brackish water of tidal ponds and channels in saltmarshes, lagoon ecosystems and river estuaries at seven sites along the Algarve coast in the South of Portugal. Phylogenetic analyses of LSU and ITS datasets, comprising all described Halophytophthora species, the 10 new Halophytophthora taxa and all relevant and distinctive sequences available from GenBank, provided an updated phylogeny of the genus Halophytophthora s.str. showing for the first time a structure of 10 clades designated as Clades 1-10. Nine of the 10 new Halophytophthora taxa resided in Clade 6 together with H. polymorphica and H. vesicula. Based on differences in morphology and temperature-growth relations and a multigene (LSU, ITS, Btub, hsp90, rpl10, tigA, cox1, nadh1, rps10) phylo-geny, eight new Halophytophthora taxa from Portugal are described here as H. brevisporangia, H. cele-ris, H. frigida, H. lateralis, H. lusitanica, H. macrosporangia, H. sinuata and H. thermoambigua. Three species, H. frigida, H. macrosporangia and H. sinuata, have a homothallic breeding system while the remaining five species are sterile. Pathogenicity and litter decomposition tests are underway to clarify their pathological and ecological role in the marine and brackish-water ecosystems. More oomycete surveys in yet undersurveyed regions of the world and population genetic or phylogenomic analyses of global populations are needed to clarify the origin of the new Halophytophthora species.",
journal = "Persoonia",
title = "Eight new Halophytophthora species from marine and brackish-water ecosystems in Portugal and an updated phylogeny for the genus",
pages = "90-54",
volume = "48",
doi = "10.3767/persoonia.2022.48.02",
url = "conv_1668"
}

Maia, C., Horta Jung, M., Carella, G., Milenković, I., Janousek, J., Tomsovsky, M., Mosca, S., Schena, L., Cravador, A., Moricca, S.,& Jung, T.. (2022). Eight new Halophytophthora species from marine and brackish-water ecosystems in Portugal and an updated phylogeny for the genus. in Persoonia, 48, 54-90.
https://doi.org/10.3767/persoonia.2022.48.02
conv_1668

Maia C, Horta Jung M, Carella G, Milenković I, Janousek J, Tomsovsky M, Mosca S, Schena L, Cravador A, Moricca S, Jung T. Eight new Halophytophthora species from marine and brackish-water ecosystems in Portugal and an updated phylogeny for the genus. in Persoonia. 2022;48:54-90.
doi:10.3767/persoonia.2022.48.02
conv_1668 .

Maia, C., Horta Jung, Marilia, Carella, G., Milenković, Ivan, Janousek, J., Tomsovsky, M., Mosca, S., Schena, L., Cravador, A., Moricca, S., Jung, T., "Eight new Halophytophthora species from marine and brackish-water ecosystems in Portugal and an updated phylogeny for the genus" in Persoonia, 48 (2022):54-90,
https://doi.org/10.3767/persoonia.2022.48.02 .,
conv_1668 .

TY  - JOUR
AU  - Milenković, Ivan
AU  - Radulović, Zlatan
AU  - Karadžić, Dragan
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1323
AB  - While monitoring the health of different ornamental and shade trees in Serbia, symptoms indicative of cypress canker disease were observed in young Cupressus sempervirens trees in the Belgrade urban area. Symptoms included tree mortality (specimens were recorded with a change in needle colour, branch decline or longitudinal bark cankers on the stems with resin exudates) and the appearance of acervuli fruiting bodies on the bark and cones. Using light microscopy, cross sections of fruiting bodies on the cankered bark and cones were examined and numerous fusoid, six-cell conidia were recorded with four inner coloured cells and two hyaline cells at the ends. Based on the unique combination of the morphological features and the infected host, this pathogen was identified as Seiridium cardinale. This is the first report of S. cardinale on the common cypress in Serbia. Possible introduction pathways and the implications of the findings are discussed.
T2  - Plant Protection Science
T1  - First report of Seiridium cardinale on Cupressus sempervirens in Serbia
EP  - 364
IS  - 4
SP  - 360
VL  - 58
DO  - 10.17221/54/2021-PPS
UR  - conv_1638
ER  - 

@article{
author = "Milenković, Ivan and Radulović, Zlatan and Karadžić, Dragan",
year = "2022",
abstract = "While monitoring the health of different ornamental and shade trees in Serbia, symptoms indicative of cypress canker disease were observed in young Cupressus sempervirens trees in the Belgrade urban area. Symptoms included tree mortality (specimens were recorded with a change in needle colour, branch decline or longitudinal bark cankers on the stems with resin exudates) and the appearance of acervuli fruiting bodies on the bark and cones. Using light microscopy, cross sections of fruiting bodies on the cankered bark and cones were examined and numerous fusoid, six-cell conidia were recorded with four inner coloured cells and two hyaline cells at the ends. Based on the unique combination of the morphological features and the infected host, this pathogen was identified as Seiridium cardinale. This is the first report of S. cardinale on the common cypress in Serbia. Possible introduction pathways and the implications of the findings are discussed.",
journal = "Plant Protection Science",
title = "First report of Seiridium cardinale on Cupressus sempervirens in Serbia",
pages = "364-360",
number = "4",
volume = "58",
doi = "10.17221/54/2021-PPS",
url = "conv_1638"
}

Milenković, I., Radulović, Z.,& Karadžić, D.. (2022). First report of Seiridium cardinale on Cupressus sempervirens in Serbia. in Plant Protection Science, 58(4), 360-364.
https://doi.org/10.17221/54/2021-PPS
conv_1638

Milenković I, Radulović Z, Karadžić D. First report of Seiridium cardinale on Cupressus sempervirens in Serbia. in Plant Protection Science. 2022;58(4):360-364.
doi:10.17221/54/2021-PPS
conv_1638 .

Milenković, Ivan, Radulović, Zlatan, Karadžić, Dragan, "First report of Seiridium cardinale on Cupressus sempervirens in Serbia" in Plant Protection Science, 58, no. 4 (2022):360-364,
https://doi.org/10.17221/54/2021-PPS .,
conv_1638 .

TY  - JOUR
AU  - Cerny, Martin
AU  - Berka, Miroslav
AU  - Dvorak, Milon
AU  - Milenković, Ivan
AU  - Saiz-Fernandez, Inigo
AU  - Brzobohaty, Bretislav
AU  - Durković, Jaroslav
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1295
AB  - Poplars are among the fastest-growing trees and significant resources in agriculture and forestry. However, rapid growth requires a large water consumption, and irrigation water provides a natural means for pathogen spread. That includes members of Phytophthora spp. that have proven to be a global enemy to forests. With the known adaptability to new hosts, it is only a matter of time for more aggressive Phytophthora species to become a threat to poplar forests and plantations. Here, the effects of artificial inoculation with two different representatives of aggressive species (P. cactorum and P. plurivora) were analyzed in the proteome of the Phytophthora-tolerant hybrid poplar clone T-14 [Populus tremula L. 70 x (Populus x canescens (Ait.) Sm. 23)]. Wood microcore samples were collected at the active necrosis borders to provide insight into the molecular processes underlying the observed tolerance to Phytophthora. The analysis revealed the impact of Phytophthora on poplar primary and secondary metabolism, including carbohydrate-active enzymes, amino acid biosynthesis, phenolic metabolism, and lipid metabolism, all of which were confirmed by consecutive metabolome and lipidome profiling. Modulations of enzymes indicating systemic response were confirmed by the analysis of leaf proteome, and sampling of wood microcores in distal locations revealed proteins with abundance correlating with proximity to the infection, including germin-like proteins, components of proteosynthesis, glutamate carboxypeptidase, and an enzyme that likely promotes anthocyanin stability. Finally, the identified Phytophthora-responsive proteins were compared to those previously found in trees with compromised defense against Phytophthora, namely, Quercus spp. and Castanea sativa. That provided a subset of candidate markers of Phytophthora tolerance, including certain ribosomal proteins, auxin metabolism enzymes, dioxygenases, polyphenol oxidases, trehalose-phosphate synthase, mannose-1-phosphate guanylyltransferase, and rhamnose biosynthetic enzymes. In summary, this analysis provided the first insight into the molecular mechanisms of hybrid poplar defense against Phytophthora and identified prospective targets for improving Phytophthora tolerance in trees.
T2  - Frontiers in Plant Science
T1  - Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar
VL  - 13
DO  - 10.3389/fpls.2022.1018272
UR  - conv_1666
ER  - 

@article{
author = "Cerny, Martin and Berka, Miroslav and Dvorak, Milon and Milenković, Ivan and Saiz-Fernandez, Inigo and Brzobohaty, Bretislav and Durković, Jaroslav",
year = "2022",
abstract = "Poplars are among the fastest-growing trees and significant resources in agriculture and forestry. However, rapid growth requires a large water consumption, and irrigation water provides a natural means for pathogen spread. That includes members of Phytophthora spp. that have proven to be a global enemy to forests. With the known adaptability to new hosts, it is only a matter of time for more aggressive Phytophthora species to become a threat to poplar forests and plantations. Here, the effects of artificial inoculation with two different representatives of aggressive species (P. cactorum and P. plurivora) were analyzed in the proteome of the Phytophthora-tolerant hybrid poplar clone T-14 [Populus tremula L. 70 x (Populus x canescens (Ait.) Sm. 23)]. Wood microcore samples were collected at the active necrosis borders to provide insight into the molecular processes underlying the observed tolerance to Phytophthora. The analysis revealed the impact of Phytophthora on poplar primary and secondary metabolism, including carbohydrate-active enzymes, amino acid biosynthesis, phenolic metabolism, and lipid metabolism, all of which were confirmed by consecutive metabolome and lipidome profiling. Modulations of enzymes indicating systemic response were confirmed by the analysis of leaf proteome, and sampling of wood microcores in distal locations revealed proteins with abundance correlating with proximity to the infection, including germin-like proteins, components of proteosynthesis, glutamate carboxypeptidase, and an enzyme that likely promotes anthocyanin stability. Finally, the identified Phytophthora-responsive proteins were compared to those previously found in trees with compromised defense against Phytophthora, namely, Quercus spp. and Castanea sativa. That provided a subset of candidate markers of Phytophthora tolerance, including certain ribosomal proteins, auxin metabolism enzymes, dioxygenases, polyphenol oxidases, trehalose-phosphate synthase, mannose-1-phosphate guanylyltransferase, and rhamnose biosynthetic enzymes. In summary, this analysis provided the first insight into the molecular mechanisms of hybrid poplar defense against Phytophthora and identified prospective targets for improving Phytophthora tolerance in trees.",
journal = "Frontiers in Plant Science",
title = "Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar",
volume = "13",
doi = "10.3389/fpls.2022.1018272",
url = "conv_1666"
}

Cerny, M., Berka, M., Dvorak, M., Milenković, I., Saiz-Fernandez, I., Brzobohaty, B.,& Durković, J.. (2022). Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar. in Frontiers in Plant Science, 13.
https://doi.org/10.3389/fpls.2022.1018272
conv_1666

Cerny M, Berka M, Dvorak M, Milenković I, Saiz-Fernandez I, Brzobohaty B, Durković J. Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar. in Frontiers in Plant Science. 2022;13.
doi:10.3389/fpls.2022.1018272
conv_1666 .

Cerny, Martin, Berka, Miroslav, Dvorak, Milon, Milenković, Ivan, Saiz-Fernandez, Inigo, Brzobohaty, Bretislav, Durković, Jaroslav, "Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar" in Frontiers in Plant Science, 13 (2022),
https://doi.org/10.3389/fpls.2022.1018272 .,
conv_1666 .

TY  - JOUR
AU  - Nikolić, Biljana M.
AU  - Milanović, Slobodan
AU  - Milenković, Ivan
AU  - Todosijević, Marina M.
AU  - Đorđević, Iris . Z.
AU  - Brkić, Milana Z.
AU  - Mitić, Zorica S.
AU  - Marin, Petar D.
AU  - Tesević, Vele V.
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1364
AB  - We examined essential oils (EOs) of Chamaecyparis lawsoniana and Thuja plicata (Cupressaceae), their antifeedant activity on Lymanthria dispar larvae and their antimicrobial activity. Studies of EOs showed that these two conifer species differed both in content of terpene classes and the major compounds. Total monoterpenes strongly dominated in T. plicata (96.4%), while C. lawsoniana was rich in both mono- and sesquiterpenes (40.8% and 30.3%, respectively) as well as in diterpenes (19.1%). The most dominant compounds of C. lawsoniana EO were: limonene (16.7%), oplopanonyl acetate (14.5%), beyerene (10.1%), and 13-epi-dolabradiene (6.7%). The dominant compound of T. plicata EOs was alpha-thujone (76.9%), followed by relatively small amounts of beta- thujone (5.3%), sabinene (4.5%) and terpinene-4-ol (3.2%). The difference in EO compositions of the conifers was reflected on Lymantria dispar larvae performance. Larvae fed on the leaf discs treated by C. lawsoniana EO had a slight phagostimulatory effect at lower concentration shown by higher relative rate of food consumption and relative growth rate than the larvae in the control group. Contrastingly, leaf discs treated with EO of T. plicata EO had an antifeedant effect and lower relative consumption rate (RCR) and relative growth rate RGR than the larvae in the control group. Both tested EOs influenced substantially the colony growth of the subjected Phytophthora plurivora and P. quercina. Namely, 100% inhibitory effect was recorded at concentration of 0.1% in the case of C. lawsoniana EO, whereas of T. plicata colonies did not grow at 0.5% concentration. The implications of these findings and possibility of using the tested EOs in further experiments in vitro and in vivo are discussed.
T2  - Industrial Crops and Products
T1  - Bioactivity of Chamaecyparis lawsoniana (A. Murray) Parl. and Thuja plicata Donn ex D. Don essential oils on Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae) larvae and Phytophthora de Bary 1876 root pathogens
VL  - 178
DO  - 10.1016/j.indcrop.2022.114550
UR  - conv_1635
ER  - 

@article{
author = "Nikolić, Biljana M. and Milanović, Slobodan and Milenković, Ivan and Todosijević, Marina M. and Đorđević, Iris . Z. and Brkić, Milana Z. and Mitić, Zorica S. and Marin, Petar D. and Tesević, Vele V.",
year = "2022",
abstract = "We examined essential oils (EOs) of Chamaecyparis lawsoniana and Thuja plicata (Cupressaceae), their antifeedant activity on Lymanthria dispar larvae and their antimicrobial activity. Studies of EOs showed that these two conifer species differed both in content of terpene classes and the major compounds. Total monoterpenes strongly dominated in T. plicata (96.4%), while C. lawsoniana was rich in both mono- and sesquiterpenes (40.8% and 30.3%, respectively) as well as in diterpenes (19.1%). The most dominant compounds of C. lawsoniana EO were: limonene (16.7%), oplopanonyl acetate (14.5%), beyerene (10.1%), and 13-epi-dolabradiene (6.7%). The dominant compound of T. plicata EOs was alpha-thujone (76.9%), followed by relatively small amounts of beta- thujone (5.3%), sabinene (4.5%) and terpinene-4-ol (3.2%). The difference in EO compositions of the conifers was reflected on Lymantria dispar larvae performance. Larvae fed on the leaf discs treated by C. lawsoniana EO had a slight phagostimulatory effect at lower concentration shown by higher relative rate of food consumption and relative growth rate than the larvae in the control group. Contrastingly, leaf discs treated with EO of T. plicata EO had an antifeedant effect and lower relative consumption rate (RCR) and relative growth rate RGR than the larvae in the control group. Both tested EOs influenced substantially the colony growth of the subjected Phytophthora plurivora and P. quercina. Namely, 100% inhibitory effect was recorded at concentration of 0.1% in the case of C. lawsoniana EO, whereas of T. plicata colonies did not grow at 0.5% concentration. The implications of these findings and possibility of using the tested EOs in further experiments in vitro and in vivo are discussed.",
journal = "Industrial Crops and Products",
title = "Bioactivity of Chamaecyparis lawsoniana (A. Murray) Parl. and Thuja plicata Donn ex D. Don essential oils on Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae) larvae and Phytophthora de Bary 1876 root pathogens",
volume = "178",
doi = "10.1016/j.indcrop.2022.114550",
url = "conv_1635"
}

Nikolić, B. M., Milanović, S., Milenković, I., Todosijević, M. M., Đorđević, I. . Z., Brkić, M. Z., Mitić, Z. S., Marin, P. D.,& Tesević, V. V.. (2022). Bioactivity of Chamaecyparis lawsoniana (A. Murray) Parl. and Thuja plicata Donn ex D. Don essential oils on Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae) larvae and Phytophthora de Bary 1876 root pathogens. in Industrial Crops and Products, 178.
https://doi.org/10.1016/j.indcrop.2022.114550
conv_1635

Nikolić BM, Milanović S, Milenković I, Todosijević MM, Đorđević I.Z, Brkić MZ, Mitić ZS, Marin PD, Tesević VV. Bioactivity of Chamaecyparis lawsoniana (A. Murray) Parl. and Thuja plicata Donn ex D. Don essential oils on Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae) larvae and Phytophthora de Bary 1876 root pathogens. in Industrial Crops and Products. 2022;178.
doi:10.1016/j.indcrop.2022.114550
conv_1635 .

Nikolić, Biljana M., Milanović, Slobodan, Milenković, Ivan, Todosijević, Marina M., Đorđević, Iris . Z., Brkić, Milana Z., Mitić, Zorica S., Marin, Petar D., Tesević, Vele V., "Bioactivity of Chamaecyparis lawsoniana (A. Murray) Parl. and Thuja plicata Donn ex D. Don essential oils on Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae) larvae and Phytophthora de Bary 1876 root pathogens" in Industrial Crops and Products, 178 (2022),
https://doi.org/10.1016/j.indcrop.2022.114550 .,
conv_1635 .

TY  - JOUR
AU  - O'Hanlon, Richard
AU  - Destefanis, Maria
AU  - Milenković, Ivan
AU  - Tomsovsky, Michal
AU  - Janousek, Josef
AU  - Bellgard, Stanley E.
AU  - Weir, Bevan S.
AU  - Kudlacek, Tomas
AU  - Horta Jung, Marilia
AU  - Jung, Thomas
PY  - 2021
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1184
AB  - Slow growing oomycete isolates with morphological resemblance to Phytophthora were obtained from forest streams during routine monitoring for the EU quarantine forest pathogen Phytophthora ramorum in Ireland and Northern Ireland. Internal Transcribed Spacer (ITS) sequence analysis indicated that they belonged to two previously unknown species of Nothophytophthora, a recently erected sister genus of Phytophthora. Morphological and temperature-growth studies were carried out to characterise both new species. In addition, Bayesian and Maximum-Likelihood analyses of nuclear 5-loci and mitochondrial 3-loci datasets were performed to resolve the phylogenetic positions of the two new species. Both species were sterile, formed chlamydospores and partially caducous nonpapillate sporangia, and showed slower growth than any of the six known Nothophytophthora species. In all phylogenetic analyses both species formed distinct, strongly supported clades, closely related to N. chlamydospora and N. valdiviana from Chile. Based on their unique combination of morphological and physiological characters and their distinct phylogenetic positions the two new species are described as Nothophytophthora irlandica sp. nov. and N. lirii sp. nov. Their potential lifestyle and geographic origin are discussed.
T2  - PLoS One
T1  - Two new Nothophytophthora species from streams in Ireland and Northern Ireland: Nothophytophthora irlandica and N . lirii sp. nov.
IS  - 5
VL  - 16
DO  - 10.1371/journal.pone.0250527
UR  - conv_1557
ER  - 

@article{
author = "O'Hanlon, Richard and Destefanis, Maria and Milenković, Ivan and Tomsovsky, Michal and Janousek, Josef and Bellgard, Stanley E. and Weir, Bevan S. and Kudlacek, Tomas and Horta Jung, Marilia and Jung, Thomas",
year = "2021",
abstract = "Slow growing oomycete isolates with morphological resemblance to Phytophthora were obtained from forest streams during routine monitoring for the EU quarantine forest pathogen Phytophthora ramorum in Ireland and Northern Ireland. Internal Transcribed Spacer (ITS) sequence analysis indicated that they belonged to two previously unknown species of Nothophytophthora, a recently erected sister genus of Phytophthora. Morphological and temperature-growth studies were carried out to characterise both new species. In addition, Bayesian and Maximum-Likelihood analyses of nuclear 5-loci and mitochondrial 3-loci datasets were performed to resolve the phylogenetic positions of the two new species. Both species were sterile, formed chlamydospores and partially caducous nonpapillate sporangia, and showed slower growth than any of the six known Nothophytophthora species. In all phylogenetic analyses both species formed distinct, strongly supported clades, closely related to N. chlamydospora and N. valdiviana from Chile. Based on their unique combination of morphological and physiological characters and their distinct phylogenetic positions the two new species are described as Nothophytophthora irlandica sp. nov. and N. lirii sp. nov. Their potential lifestyle and geographic origin are discussed.",
journal = "PLoS One",
title = "Two new Nothophytophthora species from streams in Ireland and Northern Ireland: Nothophytophthora irlandica and N . lirii sp. nov.",
number = "5",
volume = "16",
doi = "10.1371/journal.pone.0250527",
url = "conv_1557"
}

O'Hanlon, R., Destefanis, M., Milenković, I., Tomsovsky, M., Janousek, J., Bellgard, S. E., Weir, B. S., Kudlacek, T., Horta Jung, M.,& Jung, T.. (2021). Two new Nothophytophthora species from streams in Ireland and Northern Ireland: Nothophytophthora irlandica and N . lirii sp. nov.. in PLoS One, 16(5).
https://doi.org/10.1371/journal.pone.0250527
conv_1557

O'Hanlon R, Destefanis M, Milenković I, Tomsovsky M, Janousek J, Bellgard SE, Weir BS, Kudlacek T, Horta Jung M, Jung T. Two new Nothophytophthora species from streams in Ireland and Northern Ireland: Nothophytophthora irlandica and N . lirii sp. nov.. in PLoS One. 2021;16(5).
doi:10.1371/journal.pone.0250527
conv_1557 .

O'Hanlon, Richard, Destefanis, Maria, Milenković, Ivan, Tomsovsky, Michal, Janousek, Josef, Bellgard, Stanley E., Weir, Bevan S., Kudlacek, Tomas, Horta Jung, Marilia, Jung, Thomas, "Two new Nothophytophthora species from streams in Ireland and Northern Ireland: Nothophytophthora irlandica and N . lirii sp. nov." in PLoS One, 16, no. 5 (2021),
https://doi.org/10.1371/journal.pone.0250527 .,
conv_1557 .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Milenković, Ivan
AU  - Dobrosavljević, Jovan
AU  - Popović, Marija
AU  - Solla, Alejandro
AU  - Tomsovsky, Michal
AU  - Jankovsky, Libor
PY  - 2020
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1140
AB  - Interactions between plants, insects and pathogens are complex and not sufficiently understood in the context of climate change. In this study, the impact of a root pathogen on a leaf-eating insect hosted by a tree species at elevated CO2 concentration is reported for the first time. The combined and isolated effects of CO2 and infection by the root pathogen Phytophthora plurivora on English oak (Quercus robur) seedlings were used to assess growth rates of plants and of gypsy moth (Lymantria dispar) larvae. For this purpose, two Q. robur provenances (Belgrade and Sombor) were used. At ambient CO2 concentration, the relative growth rates of larvae consuming leaves of plants infected by P. plurivora was higher than those consuming non-infected plants. However, at elevated CO2 concentration (1000 ppm) higher relative growth rates were detected in the larvae consuming the leaves of non-infected plants. At ambient CO2 concentration, lower growth rates were recorded in L. dispar larvae hosted in Q. robur from Belgrade in comparison to larvae hosted in Q. robur from Sombor. However, at elevated CO2 concentration, similar growth rates irrespective of the provenance were observed. Defoliation by the gypsy moth did not influence the growth of plants while P. plurivora infection significantly reduced tree height in seedlings from Belgrade. The results confirm that a rise of CO2 concentration in the atmosphere modifies the existing interactions between P. plurivora, Q. robur, and L. dispar. Moreover, the influence of the tree provenances on both herbivore and plant performance at elevated CO2 concentrations suggests a potential for increasing forest resilience through breeding.
T2  - Forests
T1  - Growth Rates of Lymantria dispar Larvae and Quercus robur Seedlings at Elevated CO 2 Concentration and Phytophthora plurivora Infection
IS  - 10
VL  - 11
DO  - 10.3390/f11101059
UR  - conv_1509
ER  - 

@article{
author = "Milanović, Slobodan and Milenković, Ivan and Dobrosavljević, Jovan and Popović, Marija and Solla, Alejandro and Tomsovsky, Michal and Jankovsky, Libor",
year = "2020",
abstract = "Interactions between plants, insects and pathogens are complex and not sufficiently understood in the context of climate change. In this study, the impact of a root pathogen on a leaf-eating insect hosted by a tree species at elevated CO2 concentration is reported for the first time. The combined and isolated effects of CO2 and infection by the root pathogen Phytophthora plurivora on English oak (Quercus robur) seedlings were used to assess growth rates of plants and of gypsy moth (Lymantria dispar) larvae. For this purpose, two Q. robur provenances (Belgrade and Sombor) were used. At ambient CO2 concentration, the relative growth rates of larvae consuming leaves of plants infected by P. plurivora was higher than those consuming non-infected plants. However, at elevated CO2 concentration (1000 ppm) higher relative growth rates were detected in the larvae consuming the leaves of non-infected plants. At ambient CO2 concentration, lower growth rates were recorded in L. dispar larvae hosted in Q. robur from Belgrade in comparison to larvae hosted in Q. robur from Sombor. However, at elevated CO2 concentration, similar growth rates irrespective of the provenance were observed. Defoliation by the gypsy moth did not influence the growth of plants while P. plurivora infection significantly reduced tree height in seedlings from Belgrade. The results confirm that a rise of CO2 concentration in the atmosphere modifies the existing interactions between P. plurivora, Q. robur, and L. dispar. Moreover, the influence of the tree provenances on both herbivore and plant performance at elevated CO2 concentrations suggests a potential for increasing forest resilience through breeding.",
journal = "Forests",
title = "Growth Rates of Lymantria dispar Larvae and Quercus robur Seedlings at Elevated CO 2 Concentration and Phytophthora plurivora Infection",
number = "10",
volume = "11",
doi = "10.3390/f11101059",
url = "conv_1509"
}

Milanović, S., Milenković, I., Dobrosavljević, J., Popović, M., Solla, A., Tomsovsky, M.,& Jankovsky, L.. (2020). Growth Rates of Lymantria dispar Larvae and Quercus robur Seedlings at Elevated CO 2 Concentration and Phytophthora plurivora Infection. in Forests, 11(10).
https://doi.org/10.3390/f11101059
conv_1509

Milanović S, Milenković I, Dobrosavljević J, Popović M, Solla A, Tomsovsky M, Jankovsky L. Growth Rates of Lymantria dispar Larvae and Quercus robur Seedlings at Elevated CO 2 Concentration and Phytophthora plurivora Infection. in Forests. 2020;11(10).
doi:10.3390/f11101059
conv_1509 .

Milanović, Slobodan, Milenković, Ivan, Dobrosavljević, Jovan, Popović, Marija, Solla, Alejandro, Tomsovsky, Michal, Jankovsky, Libor, "Growth Rates of Lymantria dispar Larvae and Quercus robur Seedlings at Elevated CO 2 Concentration and Phytophthora plurivora Infection" in Forests, 11, no. 10 (2020),
https://doi.org/10.3390/f11101059 .,
conv_1509 .

TY  - JOUR
AU  - Jung, Thomas
AU  - Scanu, Bruno
AU  - Brasier, Clive M.
AU  - Webber, Joan
AU  - Milenković, Ivan
AU  - Corcobado, Tamara
AU  - Tomsovsky, Michal
AU  - Panek, Matej
AU  - Bakonyi, Jozsef
AU  - Maia, Cristiana
AU  - Bacova, Aneta
AU  - Raco, Milica
AU  - Rees, Helen
AU  - Perez-Sierra, Ana
AU  - Horta Jung, Marilia
PY  - 2020
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1137
AB  - In 2016 and 2017, surveys of Phytophthora diversity were performed in 25 natural and semi-natural forest stands and 16 rivers in temperate and subtropical montane and tropical lowland regions of Vietnam. Using baiting assays from soil samples and rivers and direct isolations from naturally fallen leaves, 13 described species, five informally designated taxa and 21 previously unknown taxa of Phytophthora were isolated from 58 of the 91 soil samples (63.7%) taken from the rhizosphere of 52 of the 64 woody plant species sampled (81.3%) in 20 forest stands (83.7%), and from all rivers: P. capensis, P. citricola VII, VIII, IX, X and XI, P. sp. botryosa-like 2, P. sp. meadii-like 1 and 2, P. sp. tropicalis-like 2 and P. sp. multivesiculata-like 1 from Phytophthora major phylogenetic Clade 2; P. castaneae and P. heveae from Clade 5; P. chlamydospora, P. gregata, P. sp. bitahaiensis-like and P. sp. sylvatica-like 1, 2 and 3 from Clade 6; P. cinnamomi (Pc), P. parvispora, P. attenuata, P. sp. attenuata-like 1, 2 and 3 and P. xheterohybrida from Clade 7; P. drechsleri, P. pseudocryptogea, P. ramorum (Pr) and P. sp. kelmania from Clade 8, P. macrochlamydospora, P. sp. xinsolita-like, P. sp. xkunnunara-like, P. sp. xvirginiana-like s.l. and three new taxa, P. sp. quininea-like, P. sp. xGrenada 3-like and P. sp. xPeru 4-like, from Clade 9; and P. sp. gallica-like 1 and 2 from Clade 10. The Al and A2 mating types of both Pc and Pr co-occurred. The A2 mating type of Pc was associated with severe dieback of montane forests in northern Vietnam. Most other Phytophthora species, including Pr, were not associated with obvious disease symptoms. It is concluded that (1) Vietnam is within the center of origin of most Phytophthora taxa found including Pc and Pr, and (2) Phytophthora clades 2, 5, 6, 7, 8, 9, and 10 are native to Indochina.
T2  - Forests
T1  - A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum
IS  - 1
VL  - 11
DO  - 10.3390/f11010093
UR  - conv_1479
ER  - 

@article{
author = "Jung, Thomas and Scanu, Bruno and Brasier, Clive M. and Webber, Joan and Milenković, Ivan and Corcobado, Tamara and Tomsovsky, Michal and Panek, Matej and Bakonyi, Jozsef and Maia, Cristiana and Bacova, Aneta and Raco, Milica and Rees, Helen and Perez-Sierra, Ana and Horta Jung, Marilia",
year = "2020",
abstract = "In 2016 and 2017, surveys of Phytophthora diversity were performed in 25 natural and semi-natural forest stands and 16 rivers in temperate and subtropical montane and tropical lowland regions of Vietnam. Using baiting assays from soil samples and rivers and direct isolations from naturally fallen leaves, 13 described species, five informally designated taxa and 21 previously unknown taxa of Phytophthora were isolated from 58 of the 91 soil samples (63.7%) taken from the rhizosphere of 52 of the 64 woody plant species sampled (81.3%) in 20 forest stands (83.7%), and from all rivers: P. capensis, P. citricola VII, VIII, IX, X and XI, P. sp. botryosa-like 2, P. sp. meadii-like 1 and 2, P. sp. tropicalis-like 2 and P. sp. multivesiculata-like 1 from Phytophthora major phylogenetic Clade 2; P. castaneae and P. heveae from Clade 5; P. chlamydospora, P. gregata, P. sp. bitahaiensis-like and P. sp. sylvatica-like 1, 2 and 3 from Clade 6; P. cinnamomi (Pc), P. parvispora, P. attenuata, P. sp. attenuata-like 1, 2 and 3 and P. xheterohybrida from Clade 7; P. drechsleri, P. pseudocryptogea, P. ramorum (Pr) and P. sp. kelmania from Clade 8, P. macrochlamydospora, P. sp. xinsolita-like, P. sp. xkunnunara-like, P. sp. xvirginiana-like s.l. and three new taxa, P. sp. quininea-like, P. sp. xGrenada 3-like and P. sp. xPeru 4-like, from Clade 9; and P. sp. gallica-like 1 and 2 from Clade 10. The Al and A2 mating types of both Pc and Pr co-occurred. The A2 mating type of Pc was associated with severe dieback of montane forests in northern Vietnam. Most other Phytophthora species, including Pr, were not associated with obvious disease symptoms. It is concluded that (1) Vietnam is within the center of origin of most Phytophthora taxa found including Pc and Pr, and (2) Phytophthora clades 2, 5, 6, 7, 8, 9, and 10 are native to Indochina.",
journal = "Forests",
title = "A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum",
number = "1",
volume = "11",
doi = "10.3390/f11010093",
url = "conv_1479"
}

Jung, T., Scanu, B., Brasier, C. M., Webber, J., Milenković, I., Corcobado, T., Tomsovsky, M., Panek, M., Bakonyi, J., Maia, C., Bacova, A., Raco, M., Rees, H., Perez-Sierra, A.,& Horta Jung, M.. (2020). A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum. in Forests, 11(1).
https://doi.org/10.3390/f11010093
conv_1479

Jung T, Scanu B, Brasier CM, Webber J, Milenković I, Corcobado T, Tomsovsky M, Panek M, Bakonyi J, Maia C, Bacova A, Raco M, Rees H, Perez-Sierra A, Horta Jung M. A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum. in Forests. 2020;11(1).
doi:10.3390/f11010093
conv_1479 .

Jung, Thomas, Scanu, Bruno, Brasier, Clive M., Webber, Joan, Milenković, Ivan, Corcobado, Tamara, Tomsovsky, Michal, Panek, Matej, Bakonyi, Jozsef, Maia, Cristiana, Bacova, Aneta, Raco, Milica, Rees, Helen, Perez-Sierra, Ana, Horta Jung, Marilia, "A Survey in Natural Forest Ecosystems of Vietnam Reveals High Diversity of both New and Described Phytophthora Taxa including P . ramorum" in Forests, 11, no. 1 (2020),
https://doi.org/10.3390/f11010093 .,
conv_1479 .

TY  - JOUR
AU  - Karadžić, Dragan
AU  - Stanivuković, Zoran
AU  - Milanović, Slobodan
AU  - Sikora, Katarzyna
AU  - Radulović, Zlatan
AU  - Racko, Vladimir
AU  - Kardosova, Monika
AU  - Durković, Jaroslav
AU  - Milenković, Ivan
PY  - 2020
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1145
AB  - When monitoring the state of health of Fraxinus excelsior trees, unusual symptoms were discovered within a F. excelsior plantation in Bosnia and Herzegovina. These symptoms included the appearance of necrosis and cankers in the basal parts of the trees, followed by the formation of fruiting bodies, however, none of these symptoms were found in the crowns. After sampling and isolation of the necrotic parts from the stem base, pathogen Neonectria punicea was isolated and identified from the characteristics of pure cultures, morphology of the fruiting bodies, and from multilocus sequencing. In field conditions, juvenile F. excelsior trees were inoculated with two N. punicea isolates obtained from the necrotic tissues of both juvenile F. excelsior and mature Fagus sylvatica trees. In both isolates, 12 months post inoculation, the lengths and widths of the necroses were significantly larger compared to the control. Necroses of significantly larger lengths, widths and surfaces were found again in both tested isolates 24 months post inoculation. In the case of the F. excelsior isolate, the lengths of the necroses at both the stem base and at breast height increased by 1.6 times, whereas the F. sylvatica isolate increased in size by up to 1.7 and 1.8 times, respectively. Trees inoculated without a previous bark wound showed no symptoms, similar to the control trees. Scanning electron microscopy and X-ray micro-computed tomography imaging revealed that N. punicea hyphae penetrated from the cankers to the woody outermost annual growth ring and that hyphae were present mostly in the large earlywood vessels and rarely in the axial parenchyma cells. Hyphae also spread radially through the pits in vessels. The infected trees responded with the formation of tyloses in the vessels to prevent a rapid fungal spread through the axial vascular transport pathway. The ability of N. punicea to cause necroses in juvenile ash trees was demonstrated for the first time during this study. It poses a serious threat to planted forests and natural regenerations of F. excelsior especially if F. sylvatica is considered as a possible inoculum reservoir for future infections. This pathogen should be integrated within future ash resistance or breeding programs.
T2  - Frontiers in Plant Science
T1  - Development of Neonectria punicea Pathogenic Symptoms in Juvenile Fraxinus excelsior Trees
VL  - 11
DO  - 10.3389/fpls.2020.592260
UR  - conv_1521
ER  - 

@article{
author = "Karadžić, Dragan and Stanivuković, Zoran and Milanović, Slobodan and Sikora, Katarzyna and Radulović, Zlatan and Racko, Vladimir and Kardosova, Monika and Durković, Jaroslav and Milenković, Ivan",
year = "2020",
abstract = "When monitoring the state of health of Fraxinus excelsior trees, unusual symptoms were discovered within a F. excelsior plantation in Bosnia and Herzegovina. These symptoms included the appearance of necrosis and cankers in the basal parts of the trees, followed by the formation of fruiting bodies, however, none of these symptoms were found in the crowns. After sampling and isolation of the necrotic parts from the stem base, pathogen Neonectria punicea was isolated and identified from the characteristics of pure cultures, morphology of the fruiting bodies, and from multilocus sequencing. In field conditions, juvenile F. excelsior trees were inoculated with two N. punicea isolates obtained from the necrotic tissues of both juvenile F. excelsior and mature Fagus sylvatica trees. In both isolates, 12 months post inoculation, the lengths and widths of the necroses were significantly larger compared to the control. Necroses of significantly larger lengths, widths and surfaces were found again in both tested isolates 24 months post inoculation. In the case of the F. excelsior isolate, the lengths of the necroses at both the stem base and at breast height increased by 1.6 times, whereas the F. sylvatica isolate increased in size by up to 1.7 and 1.8 times, respectively. Trees inoculated without a previous bark wound showed no symptoms, similar to the control trees. Scanning electron microscopy and X-ray micro-computed tomography imaging revealed that N. punicea hyphae penetrated from the cankers to the woody outermost annual growth ring and that hyphae were present mostly in the large earlywood vessels and rarely in the axial parenchyma cells. Hyphae also spread radially through the pits in vessels. The infected trees responded with the formation of tyloses in the vessels to prevent a rapid fungal spread through the axial vascular transport pathway. The ability of N. punicea to cause necroses in juvenile ash trees was demonstrated for the first time during this study. It poses a serious threat to planted forests and natural regenerations of F. excelsior especially if F. sylvatica is considered as a possible inoculum reservoir for future infections. This pathogen should be integrated within future ash resistance or breeding programs.",
journal = "Frontiers in Plant Science",
title = "Development of Neonectria punicea Pathogenic Symptoms in Juvenile Fraxinus excelsior Trees",
volume = "11",
doi = "10.3389/fpls.2020.592260",
url = "conv_1521"
}

Karadžić, D., Stanivuković, Z., Milanović, S., Sikora, K., Radulović, Z., Racko, V., Kardosova, M., Durković, J.,& Milenković, I.. (2020). Development of Neonectria punicea Pathogenic Symptoms in Juvenile Fraxinus excelsior Trees. in Frontiers in Plant Science, 11.
https://doi.org/10.3389/fpls.2020.592260
conv_1521

Karadžić D, Stanivuković Z, Milanović S, Sikora K, Radulović Z, Racko V, Kardosova M, Durković J, Milenković I. Development of Neonectria punicea Pathogenic Symptoms in Juvenile Fraxinus excelsior Trees. in Frontiers in Plant Science. 2020;11.
doi:10.3389/fpls.2020.592260
conv_1521 .

Karadžić, Dragan, Stanivuković, Zoran, Milanović, Slobodan, Sikora, Katarzyna, Radulović, Zlatan, Racko, Vladimir, Kardosova, Monika, Durković, Jaroslav, Milenković, Ivan, "Development of Neonectria punicea Pathogenic Symptoms in Juvenile Fraxinus excelsior Trees" in Frontiers in Plant Science, 11 (2020),
https://doi.org/10.3389/fpls.2020.592260 .,
conv_1521 .

TY  - JOUR
AU  - Karadžić, Dragan
AU  - Radulović, Zlatan
AU  - Sikora, Katarzyna
AU  - Stanivuković, Zoran
AU  - Golubović-Ćurguz, Vesna
AU  - Oszako, Tomasz
AU  - Milenković, Ivan
PY  - 2019
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1080
AB  - The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and planted stands of sweet chestnut in Serbia. The fungus was detected on the sweet chestnut in five localities and on the sessile oak in one locality. In total, 77 isolates from the sweet chestnut and five isolates from the sessile oak were obtained. Based on the culture morphology, all the obtained isolates were proven to be free from Cryphonectria (Saccardo) Saccardo & D. Saccardo hypovirus. The isolates of C. parasitica from the sweet chestnut were compatible with three different vegetative compatibility types, EU-12, EU-2, and EU-1, while the isolates from the sessile oak belonged to EU-12. After inoculation in laboratory conditions, the isolate from the sweet chestnut and sessile oak caused the decline in 88 and 76% of the sweet chestnut plants, respectively. In the case of the sessile oak, both isolates caused the decline in 52% of the plants. In field conditions, both isolates were aggressive to sessile oak trees after previous bark wounds and they were statistically significantly different compared to the control trees. The isolate from the sweet chestnut caused significantly larger cankers compared to both the isolate from the sessile oak and the control.
T2  - Plant Protection Science
T1  - Characterisation and pathogenicity of Cryphonectria parasitica on sweet chestnut and sessile oak trees in Serbia
EP  - 201
IS  - 3
SP  - 191
VL  - 55
DO  - 10.17221/38/2018-PPS
UR  - conv_1430
ER  - 

@article{
author = "Karadžić, Dragan and Radulović, Zlatan and Sikora, Katarzyna and Stanivuković, Zoran and Golubović-Ćurguz, Vesna and Oszako, Tomasz and Milenković, Ivan",
year = "2019",
abstract = "The presence of Cryphonectria parasitica (Murrill) M.E. Barr was studied in six natural and planted stands of sweet chestnut in Serbia. The fungus was detected on the sweet chestnut in five localities and on the sessile oak in one locality. In total, 77 isolates from the sweet chestnut and five isolates from the sessile oak were obtained. Based on the culture morphology, all the obtained isolates were proven to be free from Cryphonectria (Saccardo) Saccardo & D. Saccardo hypovirus. The isolates of C. parasitica from the sweet chestnut were compatible with three different vegetative compatibility types, EU-12, EU-2, and EU-1, while the isolates from the sessile oak belonged to EU-12. After inoculation in laboratory conditions, the isolate from the sweet chestnut and sessile oak caused the decline in 88 and 76% of the sweet chestnut plants, respectively. In the case of the sessile oak, both isolates caused the decline in 52% of the plants. In field conditions, both isolates were aggressive to sessile oak trees after previous bark wounds and they were statistically significantly different compared to the control trees. The isolate from the sweet chestnut caused significantly larger cankers compared to both the isolate from the sessile oak and the control.",
journal = "Plant Protection Science",
title = "Characterisation and pathogenicity of Cryphonectria parasitica on sweet chestnut and sessile oak trees in Serbia",
pages = "201-191",
number = "3",
volume = "55",
doi = "10.17221/38/2018-PPS",
url = "conv_1430"
}

Karadžić, D., Radulović, Z., Sikora, K., Stanivuković, Z., Golubović-Ćurguz, V., Oszako, T.,& Milenković, I.. (2019). Characterisation and pathogenicity of Cryphonectria parasitica on sweet chestnut and sessile oak trees in Serbia. in Plant Protection Science, 55(3), 191-201.
https://doi.org/10.17221/38/2018-PPS
conv_1430

Karadžić D, Radulović Z, Sikora K, Stanivuković Z, Golubović-Ćurguz V, Oszako T, Milenković I. Characterisation and pathogenicity of Cryphonectria parasitica on sweet chestnut and sessile oak trees in Serbia. in Plant Protection Science. 2019;55(3):191-201.
doi:10.17221/38/2018-PPS
conv_1430 .

Karadžić, Dragan, Radulović, Zlatan, Sikora, Katarzyna, Stanivuković, Zoran, Golubović-Ćurguz, Vesna, Oszako, Tomasz, Milenković, Ivan, "Characterisation and pathogenicity of Cryphonectria parasitica on sweet chestnut and sessile oak trees in Serbia" in Plant Protection Science, 55, no. 3 (2019):191-201,
https://doi.org/10.17221/38/2018-PPS .,
conv_1430 .

TY  - JOUR
AU  - Vemić, Aleksandar
AU  - Tomsovsky, Michal
AU  - Jung, Thomas
AU  - Milenković, Ivan
PY  - 2019
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1062
AB  - In addition to Hymenoscyphus fraxineus, two fungi identified as Diaporthe eres aff. and Fusarium sambucinum aff. were also isolated from necrotic bark lesions on declining one-year-old Fraxinus excelsior in a forest stand in Montenegro. To examine their involvement in ash decline, a pathogenicity test was performed using under bark inoculations on one-year-old Fraxinus excelsior. Hymenoscyphus fraxineus was included as comparison. All three fungal species proved highly pathogenic towards one-year-old seedlings although lesion sizes differed significantly between the different species. Hymenoscyphus fraxineus was most aggressive, followed by F. sambucinum aff., while D. eres aff. caused the smallest lesions. This study demonstrates for the first time the ability of isolates in the D. eres and F. sambucinum species complexes to cause decline on one-year-old common ash seedlings.
T2  - Forest Pathology
T1  - Pathogenicity of fungi associated with ash dieback symptoms of one-year-old Fraxinus excelsior in Montenegro
IS  - 5
VL  - 49
DO  - 10.1111/efp.12539
UR  - conv_1445
ER  - 

@article{
author = "Vemić, Aleksandar and Tomsovsky, Michal and Jung, Thomas and Milenković, Ivan",
year = "2019",
abstract = "In addition to Hymenoscyphus fraxineus, two fungi identified as Diaporthe eres aff. and Fusarium sambucinum aff. were also isolated from necrotic bark lesions on declining one-year-old Fraxinus excelsior in a forest stand in Montenegro. To examine their involvement in ash decline, a pathogenicity test was performed using under bark inoculations on one-year-old Fraxinus excelsior. Hymenoscyphus fraxineus was included as comparison. All three fungal species proved highly pathogenic towards one-year-old seedlings although lesion sizes differed significantly between the different species. Hymenoscyphus fraxineus was most aggressive, followed by F. sambucinum aff., while D. eres aff. caused the smallest lesions. This study demonstrates for the first time the ability of isolates in the D. eres and F. sambucinum species complexes to cause decline on one-year-old common ash seedlings.",
journal = "Forest Pathology",
title = "Pathogenicity of fungi associated with ash dieback symptoms of one-year-old Fraxinus excelsior in Montenegro",
number = "5",
volume = "49",
doi = "10.1111/efp.12539",
url = "conv_1445"
}

Vemić, A., Tomsovsky, M., Jung, T.,& Milenković, I.. (2019). Pathogenicity of fungi associated with ash dieback symptoms of one-year-old Fraxinus excelsior in Montenegro. in Forest Pathology, 49(5).
https://doi.org/10.1111/efp.12539
conv_1445

Vemić A, Tomsovsky M, Jung T, Milenković I. Pathogenicity of fungi associated with ash dieback symptoms of one-year-old Fraxinus excelsior in Montenegro. in Forest Pathology. 2019;49(5).
doi:10.1111/efp.12539
conv_1445 .

Vemić, Aleksandar, Tomsovsky, Michal, Jung, Thomas, Milenković, Ivan, "Pathogenicity of fungi associated with ash dieback symptoms of one-year-old Fraxinus excelsior in Montenegro" in Forest Pathology, 49, no. 5 (2019),
https://doi.org/10.1111/efp.12539 .,
conv_1445 .

TY  - JOUR
AU  - Milenković, Ivan
AU  - Keča, Nenad
AU  - Karadžić, Dragan
AU  - Radulović, Zlatan
AU  - Nowakowska, Justyna A.
AU  - Oszako, Tomasz
AU  - Sikora, Katarzyna
AU  - Corcobado, Tamara
AU  - Jung, Thomas
PY  - 2018
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/916
AB  - During a survey in three declining and three healthy poplar plantations in Serbia, six different Phytophthora species were obtained. Phytophthora plurivora was the most common, followed by P. pini, P. polonica, P. lacustris, P. cactorum, and P. gonapodyides. Pathogenicity of all isolated species to four-month and one-year-old cuttings of Populus hybrid clones I-214 and Pannonia, respectively, was tested using both a soil infestation and stem inoculation test. Isolates of P. polonica, P. x cambivora, P. cryptogea, and P. x serendipita from other host plants were included as a comparison. In the soil infestation test, the most aggressive species to clone I-214 were P. plurivora, P. x serendipita, and P. pini. On clone Pannonia, P. gonapodyides and P. pini were the most aggressive, both causing 100% mortality, followed by P. cactorum, P. x cambivora, and P. polonica. In the underbark inoculation test, the susceptibility of both poplar clones to the different Phytophthora species was largely similar, as in the soil infestation test, with the exception of P. polonica, which proved to be only weakly pathogenic to poplar bark. The most aggressive species to clone I-214 was P. pini, while on clone Pannonia, the longest lesions and highest disease incidence were caused by P. gonapodyides. Phytophthora cactorum and P. plurivora were pathogenic to both clones, whereas P. x cambivora showed only weak pathogenicity. The implications of these findings and possible pathways of dispersion of the pathogens are discussed.
T2  - Forests
T1  - Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia
IS  - 6
VL  - 9
DO  - 10.3390/f9060330
UR  - conv_1351
ER  - 

@article{
author = "Milenković, Ivan and Keča, Nenad and Karadžić, Dragan and Radulović, Zlatan and Nowakowska, Justyna A. and Oszako, Tomasz and Sikora, Katarzyna and Corcobado, Tamara and Jung, Thomas",
year = "2018",
abstract = "During a survey in three declining and three healthy poplar plantations in Serbia, six different Phytophthora species were obtained. Phytophthora plurivora was the most common, followed by P. pini, P. polonica, P. lacustris, P. cactorum, and P. gonapodyides. Pathogenicity of all isolated species to four-month and one-year-old cuttings of Populus hybrid clones I-214 and Pannonia, respectively, was tested using both a soil infestation and stem inoculation test. Isolates of P. polonica, P. x cambivora, P. cryptogea, and P. x serendipita from other host plants were included as a comparison. In the soil infestation test, the most aggressive species to clone I-214 were P. plurivora, P. x serendipita, and P. pini. On clone Pannonia, P. gonapodyides and P. pini were the most aggressive, both causing 100% mortality, followed by P. cactorum, P. x cambivora, and P. polonica. In the underbark inoculation test, the susceptibility of both poplar clones to the different Phytophthora species was largely similar, as in the soil infestation test, with the exception of P. polonica, which proved to be only weakly pathogenic to poplar bark. The most aggressive species to clone I-214 was P. pini, while on clone Pannonia, the longest lesions and highest disease incidence were caused by P. gonapodyides. Phytophthora cactorum and P. plurivora were pathogenic to both clones, whereas P. x cambivora showed only weak pathogenicity. The implications of these findings and possible pathways of dispersion of the pathogens are discussed.",
journal = "Forests",
title = "Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia",
number = "6",
volume = "9",
doi = "10.3390/f9060330",
url = "conv_1351"
}

Milenković, I., Keča, N., Karadžić, D., Radulović, Z., Nowakowska, J. A., Oszako, T., Sikora, K., Corcobado, T.,& Jung, T.. (2018). Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia. in Forests, 9(6).
https://doi.org/10.3390/f9060330
conv_1351

Milenković I, Keča N, Karadžić D, Radulović Z, Nowakowska JA, Oszako T, Sikora K, Corcobado T, Jung T. Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia. in Forests. 2018;9(6).
doi:10.3390/f9060330
conv_1351 .

Milenković, Ivan, Keča, Nenad, Karadžić, Dragan, Radulović, Zlatan, Nowakowska, Justyna A., Oszako, Tomasz, Sikora, Katarzyna, Corcobado, Tamara, Jung, Thomas, "Isolation and Pathogenicity of Phytophthora Species from Poplar Plantations in Serbia" in Forests, 9, no. 6 (2018),
https://doi.org/10.3390/f9060330 .,
conv_1351 .