TY  - JOUR
AU  - Bacova, Aneta
AU  - Cooke, David E. L.
AU  - Milenković, Ivan
AU  - Majek, Tomas
AU  - Nagy, Zoltan
AU  - Corcobado, Tamara
AU  - Randall, Eva
AU  - Keillor, Beatrix
AU  - Cock, Peter J. A.
AU  - Horta Jung, Marilia
AU  - Jung, Thomas
AU  - Tomsovsky, Michal
PY  - 2024
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1466
AB  - Phytophthora diversity was examined in eight forest and ornamental nurseries in the Czech Republic. A leaf baiting isolation technique and, in two nurseries, also Illumina DNA metabarcoding were used to reveal the diversity of Phytophthora in soil and irrigation water and compare the efficacy of both approaches. In total, baiting revealed the occurrence of 12 Phytophthora taxa in 59.4% of soil samples from seven (87.5%) nurseries. Additional baiting of compost was carried out in two nurseries and two Phytophthora species were recovered. Irrigation water was examined in three nurseries by baiting or by direct isolation from partially decomposed floating leaves collected from the water source, and two Phytophthora species were obtained. Illumina sequencing of soil and water samples was done in two and one nurseries, respectively. Phytophthora reads were identified as 45 Phytophthora taxa, 15 of them previously unknown taxa from Clades 6, 7, 8 and 9. Another 11 taxa belonged to known or undescribed species of the oomycete genera Globisporangium, Hyaloperonospora, Nothophytophthora, Peronospora and Plasmopara. Overall, with both techniques 50 Phytophthora taxa were detected with five taxa (P. taxon organica, P. plurivora, P. rosacearum, P. syringae and P. transitoria) being exclusively detected by baiting and 38 only by DNA metabarcoding. Particularly common records in DNA barcoding were P. cinnamomi and P. lateralis which were not isolated by baiting. Only seven species were detected by both techniques. It is recommended to use the combination of both techniques to determine true diversity of Phytophthora in managed or natural ecosystems and reveal the presence of rare or unknown Phytophthora taxa.
T2  - European Journal of Plant Pathology
T1  - Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques
EP  - 156
IS  - 1
SP  - 131
VL  - 170
DO  - 10.1007/s10658-024-02886-1
UR  - conv_1791
ER  - 

@article{
author = "Bacova, Aneta and Cooke, David E. L. and Milenković, Ivan and Majek, Tomas and Nagy, Zoltan and Corcobado, Tamara and Randall, Eva and Keillor, Beatrix and Cock, Peter J. A. and Horta Jung, Marilia and Jung, Thomas and Tomsovsky, Michal",
year = "2024",
abstract = "Phytophthora diversity was examined in eight forest and ornamental nurseries in the Czech Republic. A leaf baiting isolation technique and, in two nurseries, also Illumina DNA metabarcoding were used to reveal the diversity of Phytophthora in soil and irrigation water and compare the efficacy of both approaches. In total, baiting revealed the occurrence of 12 Phytophthora taxa in 59.4% of soil samples from seven (87.5%) nurseries. Additional baiting of compost was carried out in two nurseries and two Phytophthora species were recovered. Irrigation water was examined in three nurseries by baiting or by direct isolation from partially decomposed floating leaves collected from the water source, and two Phytophthora species were obtained. Illumina sequencing of soil and water samples was done in two and one nurseries, respectively. Phytophthora reads were identified as 45 Phytophthora taxa, 15 of them previously unknown taxa from Clades 6, 7, 8 and 9. Another 11 taxa belonged to known or undescribed species of the oomycete genera Globisporangium, Hyaloperonospora, Nothophytophthora, Peronospora and Plasmopara. Overall, with both techniques 50 Phytophthora taxa were detected with five taxa (P. taxon organica, P. plurivora, P. rosacearum, P. syringae and P. transitoria) being exclusively detected by baiting and 38 only by DNA metabarcoding. Particularly common records in DNA barcoding were P. cinnamomi and P. lateralis which were not isolated by baiting. Only seven species were detected by both techniques. It is recommended to use the combination of both techniques to determine true diversity of Phytophthora in managed or natural ecosystems and reveal the presence of rare or unknown Phytophthora taxa.",
journal = "European Journal of Plant Pathology",
title = "Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques",
pages = "156-131",
number = "1",
volume = "170",
doi = "10.1007/s10658-024-02886-1",
url = "conv_1791"
}

Bacova, A., Cooke, D. E. L., Milenković, I., Majek, T., Nagy, Z., Corcobado, T., Randall, E., Keillor, B., Cock, P. J. A., Horta Jung, M., Jung, T.,& Tomsovsky, M.. (2024). Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques. in European Journal of Plant Pathology, 170(1), 131-156.
https://doi.org/10.1007/s10658-024-02886-1
conv_1791

Bacova A, Cooke DEL, Milenković I, Majek T, Nagy Z, Corcobado T, Randall E, Keillor B, Cock PJA, Horta Jung M, Jung T, Tomsovsky M. Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques. in European Journal of Plant Pathology. 2024;170(1):131-156.
doi:10.1007/s10658-024-02886-1
conv_1791 .

Bacova, Aneta, Cooke, David E. L., Milenković, Ivan, Majek, Tomas, Nagy, Zoltan, Corcobado, Tamara, Randall, Eva, Keillor, Beatrix, Cock, Peter J. A., Horta Jung, Marilia, Jung, Thomas, Tomsovsky, Michal, "Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques" in European Journal of Plant Pathology, 170, no. 1 (2024):131-156,
https://doi.org/10.1007/s10658-024-02886-1 .,
conv_1791 .

TY  - JOUR
AU  - Majek, Tomas
AU  - Kolarik, Miroslav
AU  - Milenković, Ivan
AU  - Kasak, Josef
AU  - Foit, Jiri
AU  - Tomsovsky, Michal
PY  - 2024
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1480
AB  - A new ophiostomatoid fungus involved in the decline of walnuts was discovered in Czechia. The fungus was isolated from necrotic wood surrounding the larval galleries of Dryocoetes himalayensis, the alien bark beetle attacking walnut trees. This insect species is rather unique within hardwood-associated bark beetle species for its aggressiveness and ability to attack apparently healthy trees. The isolated fungus, associated with an exotic insect vector is described as a new species, Ophiostoma juglandis sp. nov., based on its unique morphology and phylogenetic position. The pathogenicity tests confirmed the aggressiveness of the species to both Juglans regia and J. nigra plants, manifested by formation of extensive bleeding and necrotic lesions. The symbiotic relationship between O. juglandis and D. himalayensis is expected due to massive presence of the fungal spores on surface of adult beetles in pits forming the elytral striae. In the case of hardwood-associated ophiostomatoid fungal species, phytopathogenicity is very rarely observed. The described species is exceptional in this ability, which corresponds to the aggressive behaviour of its vector. The role of the fungus in walnut tree mortality caused by D. himalayensis is discussed.
T2  - Journal of Plant Pathology
T1  - New Ophiostoma species associated with Dryocoetes himalayensis and decline of Juglans regia in Czechia
DO  - 10.1007/s42161-024-01769-y
UR  - conv_1830
ER  - 

@article{
author = "Majek, Tomas and Kolarik, Miroslav and Milenković, Ivan and Kasak, Josef and Foit, Jiri and Tomsovsky, Michal",
year = "2024",
abstract = "A new ophiostomatoid fungus involved in the decline of walnuts was discovered in Czechia. The fungus was isolated from necrotic wood surrounding the larval galleries of Dryocoetes himalayensis, the alien bark beetle attacking walnut trees. This insect species is rather unique within hardwood-associated bark beetle species for its aggressiveness and ability to attack apparently healthy trees. The isolated fungus, associated with an exotic insect vector is described as a new species, Ophiostoma juglandis sp. nov., based on its unique morphology and phylogenetic position. The pathogenicity tests confirmed the aggressiveness of the species to both Juglans regia and J. nigra plants, manifested by formation of extensive bleeding and necrotic lesions. The symbiotic relationship between O. juglandis and D. himalayensis is expected due to massive presence of the fungal spores on surface of adult beetles in pits forming the elytral striae. In the case of hardwood-associated ophiostomatoid fungal species, phytopathogenicity is very rarely observed. The described species is exceptional in this ability, which corresponds to the aggressive behaviour of its vector. The role of the fungus in walnut tree mortality caused by D. himalayensis is discussed.",
journal = "Journal of Plant Pathology",
title = "New Ophiostoma species associated with Dryocoetes himalayensis and decline of Juglans regia in Czechia",
doi = "10.1007/s42161-024-01769-y",
url = "conv_1830"
}

Majek, T., Kolarik, M., Milenković, I., Kasak, J., Foit, J.,& Tomsovsky, M.. (2024). New Ophiostoma species associated with Dryocoetes himalayensis and decline of Juglans regia in Czechia. in Journal of Plant Pathology.
https://doi.org/10.1007/s42161-024-01769-y
conv_1830

Majek T, Kolarik M, Milenković I, Kasak J, Foit J, Tomsovsky M. New Ophiostoma species associated with Dryocoetes himalayensis and decline of Juglans regia in Czechia. in Journal of Plant Pathology. 2024;.
doi:10.1007/s42161-024-01769-y
conv_1830 .

Majek, Tomas, Kolarik, Miroslav, Milenković, Ivan, Kasak, Josef, Foit, Jiri, Tomsovsky, Michal, "New Ophiostoma species associated with Dryocoetes himalayensis and decline of Juglans regia in Czechia" in Journal of Plant Pathology (2024),
https://doi.org/10.1007/s42161-024-01769-y .,
conv_1830 .

TY  - JOUR
AU  - Corcobado, Tamara
AU  - Cech, Thomas L.
AU  - Daxer, Andreas
AU  - Datkova, Henrieta
AU  - Janousek, Josef
AU  - Patra, Sneha
AU  - Jahn, Daniella
AU  - Huettler, Christine
AU  - Milenković, Ivan
AU  - Tomsovsky, Michal
AU  - Horta Jung, Marilia
AU  - Jung, Thomas
PY  - 2023
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1441
AB  - Waterways are ideal pathways for Phytophthora dispersal and potential introduction to terrestrial ecosystems. While many Phytophthora species from phylogenetic clades 6, 9 and 10 are predominant oomycetes in watercourses due to their adaptation to a lifestyle as saprotrophs and opportunistic pathogens of riparian plants, species from clades 2, 7 and 8 are predominantly soil- or airborne using aquatic habitats as temporal niches for spreading and invading terrestrial sites along the watercourses. In contrast to forest ecosystems, knowledge of Phytophthora diversity in watercourses in Central Europe is limited. Between 2014 and 2019 extensive surveys of streams and rivers were undertaken across Austria, in South Moravia, Czech Republic and Zilina province, Slovakia to unveil the diversity and distribution of Phytophthora and related oomycetes. In addition, in Austria riparian forests of black alder (Alnus glutinosa) and grey alder (A. incana) in lowlands and in the Alps were examined. A variety of Phytophthora species from clades 2, 6, 7, 8, 9 and 10 were isolated, with clade 6 species showing the widest distribution and abundance. Furthermore, interspecific clade 6 hybrids and other oomycetes such as Halophytophthora fluviatilis and undescribed Nothophytophthora spp. were also obtained. In riparian alders, symptoms of Phytophthora infections were associated with species from the P.xalni complex and P. plurivora. Phytophthora plurivora was most common in alder stands whereas P. uniformis was the oomycete species occurring at the highest altitude in alpine riparian areas.
T2  - Mycological Progress
T1  - Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe
IS  - 7
VL  - 22
DO  - 10.1007/s11557-023-01898-1
UR  - conv_1732
ER  - 

@article{
author = "Corcobado, Tamara and Cech, Thomas L. and Daxer, Andreas and Datkova, Henrieta and Janousek, Josef and Patra, Sneha and Jahn, Daniella and Huettler, Christine and Milenković, Ivan and Tomsovsky, Michal and Horta Jung, Marilia and Jung, Thomas",
year = "2023",
abstract = "Waterways are ideal pathways for Phytophthora dispersal and potential introduction to terrestrial ecosystems. While many Phytophthora species from phylogenetic clades 6, 9 and 10 are predominant oomycetes in watercourses due to their adaptation to a lifestyle as saprotrophs and opportunistic pathogens of riparian plants, species from clades 2, 7 and 8 are predominantly soil- or airborne using aquatic habitats as temporal niches for spreading and invading terrestrial sites along the watercourses. In contrast to forest ecosystems, knowledge of Phytophthora diversity in watercourses in Central Europe is limited. Between 2014 and 2019 extensive surveys of streams and rivers were undertaken across Austria, in South Moravia, Czech Republic and Zilina province, Slovakia to unveil the diversity and distribution of Phytophthora and related oomycetes. In addition, in Austria riparian forests of black alder (Alnus glutinosa) and grey alder (A. incana) in lowlands and in the Alps were examined. A variety of Phytophthora species from clades 2, 6, 7, 8, 9 and 10 were isolated, with clade 6 species showing the widest distribution and abundance. Furthermore, interspecific clade 6 hybrids and other oomycetes such as Halophytophthora fluviatilis and undescribed Nothophytophthora spp. were also obtained. In riparian alders, symptoms of Phytophthora infections were associated with species from the P.xalni complex and P. plurivora. Phytophthora plurivora was most common in alder stands whereas P. uniformis was the oomycete species occurring at the highest altitude in alpine riparian areas.",
journal = "Mycological Progress",
title = "Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe",
number = "7",
volume = "22",
doi = "10.1007/s11557-023-01898-1",
url = "conv_1732"
}

Corcobado, T., Cech, T. L., Daxer, A., Datkova, H., Janousek, J., Patra, S., Jahn, D., Huettler, C., Milenković, I., Tomsovsky, M., Horta Jung, M.,& Jung, T.. (2023). Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe. in Mycological Progress, 22(7).
https://doi.org/10.1007/s11557-023-01898-1
conv_1732

Corcobado T, Cech TL, Daxer A, Datkova H, Janousek J, Patra S, Jahn D, Huettler C, Milenković I, Tomsovsky M, Horta Jung M, Jung T. Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe. in Mycological Progress. 2023;22(7).
doi:10.1007/s11557-023-01898-1
conv_1732 .

Corcobado, Tamara, Cech, Thomas L., Daxer, Andreas, Datkova, Henrieta, Janousek, Josef, Patra, Sneha, Jahn, Daniella, Huettler, Christine, Milenković, Ivan, Tomsovsky, Michal, Horta Jung, Marilia, Jung, Thomas, "Phytophthora , Nothophytophthora and Halophytophthora diversity in rivers, streams and riparian alder ecosystems of Central Europe" in Mycological Progress, 22, no. 7 (2023),
https://doi.org/10.1007/s11557-023-01898-1 .,
conv_1732 .