TY  - JOUR
AU  - Stipoljev, Suncica
AU  - Buzan, Elena
AU  - Iacolina, Laura
AU  - Safner, Toni
AU  - Rezić, Andrea
AU  - Galov, Ana
AU  - Krizanović, Kresimir
AU  - Ambarli, Hueseyin
AU  - Arnal, MariaCruz
AU  - Babaev, Elmar
AU  - Bego, Ferdinand
AU  - Farkas, Attila
AU  - Gačić, Dragan
AU  - Lazar, Peter
AU  - Maletić, Vladimir
AU  - Markov, Georgi
AU  - Milošević, Dragana
AU  - Papaioannou, Haritakis
AU  - Scandura, Massimo
AU  - Sprem, Nikica
PY  - 2024
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1498
AB  - Major histocompatibility complex (MHC) genes are commonly used markers for monitoring adaptive genetic and evolutionary potential of species. In this study, we investigated genetic variation of the MHC class II DRB locus in the chamois genus Rupicapra by using next-generation sequencing. Sequencing of 102 samples led to the identification of 25 alleles, 11 of which are novel. The high ratio of the relative rates of nonsynonymous to synonymous mutations (dN/dS) suggests a signal of positive selection on this locus. We analyzed patterns of genetic variation within and among 2 subspecies of Northern Chamois and compared them to previously published studies using neutral markers to provide a basis for assessing the effects of demographic processes. Our analyses have shown that alleles are likely to be maintained by balancing selection in different populations with similar frequencies and that this mechanism also works in small, isolated populations that are strongly affected by genetic drift. In this study on the chamois genus Rupicapra, researchers explored genetic variation of the MHC class II DRB locus using next-generation sequencing. The research highlights a strong signal of positive selection at this locus, indicated by the high dN/dS ratio. The study also revealed that balancing selection maintains alleles across different chamois populations, including those that are small and isolated, emphasizing the evolutionary significance of this genetic mechanism. Graphical Abstract
T2  - Journal of Mammalogy
T1  - Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra
EP  - 322
IS  - 2
SP  - 312
VL  - 105
DO  - 10.1093/jmammal/gyae008
UR  - conv_1761
ER  - 

@article{
author = "Stipoljev, Suncica and Buzan, Elena and Iacolina, Laura and Safner, Toni and Rezić, Andrea and Galov, Ana and Krizanović, Kresimir and Ambarli, Hueseyin and Arnal, MariaCruz and Babaev, Elmar and Bego, Ferdinand and Farkas, Attila and Gačić, Dragan and Lazar, Peter and Maletić, Vladimir and Markov, Georgi and Milošević, Dragana and Papaioannou, Haritakis and Scandura, Massimo and Sprem, Nikica",
year = "2024",
abstract = "Major histocompatibility complex (MHC) genes are commonly used markers for monitoring adaptive genetic and evolutionary potential of species. In this study, we investigated genetic variation of the MHC class II DRB locus in the chamois genus Rupicapra by using next-generation sequencing. Sequencing of 102 samples led to the identification of 25 alleles, 11 of which are novel. The high ratio of the relative rates of nonsynonymous to synonymous mutations (dN/dS) suggests a signal of positive selection on this locus. We analyzed patterns of genetic variation within and among 2 subspecies of Northern Chamois and compared them to previously published studies using neutral markers to provide a basis for assessing the effects of demographic processes. Our analyses have shown that alleles are likely to be maintained by balancing selection in different populations with similar frequencies and that this mechanism also works in small, isolated populations that are strongly affected by genetic drift. In this study on the chamois genus Rupicapra, researchers explored genetic variation of the MHC class II DRB locus using next-generation sequencing. The research highlights a strong signal of positive selection at this locus, indicated by the high dN/dS ratio. The study also revealed that balancing selection maintains alleles across different chamois populations, including those that are small and isolated, emphasizing the evolutionary significance of this genetic mechanism. Graphical Abstract",
journal = "Journal of Mammalogy",
title = "Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra",
pages = "322-312",
number = "2",
volume = "105",
doi = "10.1093/jmammal/gyae008",
url = "conv_1761"
}

Stipoljev, S., Buzan, E., Iacolina, L., Safner, T., Rezić, A., Galov, A., Krizanović, K., Ambarli, H., Arnal, M., Babaev, E., Bego, F., Farkas, A., Gačić, D., Lazar, P., Maletić, V., Markov, G., Milošević, D., Papaioannou, H., Scandura, M.,& Sprem, N.. (2024). Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra. in Journal of Mammalogy, 105(2), 312-322.
https://doi.org/10.1093/jmammal/gyae008
conv_1761

Stipoljev S, Buzan E, Iacolina L, Safner T, Rezić A, Galov A, Krizanović K, Ambarli H, Arnal M, Babaev E, Bego F, Farkas A, Gačić D, Lazar P, Maletić V, Markov G, Milošević D, Papaioannou H, Scandura M, Sprem N. Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra. in Journal of Mammalogy. 2024;105(2):312-322.
doi:10.1093/jmammal/gyae008
conv_1761 .

Stipoljev, Suncica, Buzan, Elena, Iacolina, Laura, Safner, Toni, Rezić, Andrea, Galov, Ana, Krizanović, Kresimir, Ambarli, Hueseyin, Arnal, MariaCruz, Babaev, Elmar, Bego, Ferdinand, Farkas, Attila, Gačić, Dragan, Lazar, Peter, Maletić, Vladimir, Markov, Georgi, Milošević, Dragana, Papaioannou, Haritakis, Scandura, Massimo, Sprem, Nikica, "Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra" in Journal of Mammalogy, 105, no. 2 (2024):312-322,
https://doi.org/10.1093/jmammal/gyae008 .,
conv_1761 .

TY  - JOUR
AU  - Rezić, Andrea
AU  - Iacolina, Laura
AU  - Buzan, Elena
AU  - Safner, Toni
AU  - Bego, Ferdinand
AU  - Gačić, Dragan
AU  - Maletić, Vladimir
AU  - Markov, Georgi
AU  - Milošević, Dragana
AU  - Papaioannou, Haritakis
AU  - Sprem, Nikica
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1344
AB  - The Balkan chamois (Rupicapra rupicapra balcanica) is widespread on the Balkan Peninsula, along mountain massifs from Croatia in the north to Greece in the south and Bulgaria in the east. Knowledge on the genetic structure of Balkan chamois populations is limited and restricted to local studies. Therefore, the main objective of this study was to use nuclear (16 microsatellites) and mitochondrial (partial 376 base pairs control region) markers to investigate the genetic structure of this chamois subspecies throughout its distribution range and to obtain information on the degree of connectivity of the different (sub)populations. We extracted DNA from bone, dried skin and muscle tissue and successfully genotyped 92 individuals of Balkan chamois and sequenced the partial control region in 44 individuals. The Bayesian analysis suggested 3 genetic clusters and assigned individuals from Serbia and Bulgaria to two separate clusters, while individuals from the other countries belonged to the same cluster. Thirty new haplotypes were obtained from partial mitochondrial DNA sequences, with private haplotypes in all analyzed populations and only two haplotypes shared among populations, indicating the possibility of past translocations. The subspecies genetic composition presented here provides the necessary starting point to assess the conservation status of the Balkan chamois and allows the development of conservation strategies necessary for its sustainable management and conservation.
T2  - Conservation Genetics
T1  - The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA
EP  - 539
IS  - 3
SP  - 527
VL  - 23
DO  - 10.1007/s10592-022-01434-w
UR  - conv_1616
ER  - 

@article{
author = "Rezić, Andrea and Iacolina, Laura and Buzan, Elena and Safner, Toni and Bego, Ferdinand and Gačić, Dragan and Maletić, Vladimir and Markov, Georgi and Milošević, Dragana and Papaioannou, Haritakis and Sprem, Nikica",
year = "2022",
abstract = "The Balkan chamois (Rupicapra rupicapra balcanica) is widespread on the Balkan Peninsula, along mountain massifs from Croatia in the north to Greece in the south and Bulgaria in the east. Knowledge on the genetic structure of Balkan chamois populations is limited and restricted to local studies. Therefore, the main objective of this study was to use nuclear (16 microsatellites) and mitochondrial (partial 376 base pairs control region) markers to investigate the genetic structure of this chamois subspecies throughout its distribution range and to obtain information on the degree of connectivity of the different (sub)populations. We extracted DNA from bone, dried skin and muscle tissue and successfully genotyped 92 individuals of Balkan chamois and sequenced the partial control region in 44 individuals. The Bayesian analysis suggested 3 genetic clusters and assigned individuals from Serbia and Bulgaria to two separate clusters, while individuals from the other countries belonged to the same cluster. Thirty new haplotypes were obtained from partial mitochondrial DNA sequences, with private haplotypes in all analyzed populations and only two haplotypes shared among populations, indicating the possibility of past translocations. The subspecies genetic composition presented here provides the necessary starting point to assess the conservation status of the Balkan chamois and allows the development of conservation strategies necessary for its sustainable management and conservation.",
journal = "Conservation Genetics",
title = "The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA",
pages = "539-527",
number = "3",
volume = "23",
doi = "10.1007/s10592-022-01434-w",
url = "conv_1616"
}

Rezić, A., Iacolina, L., Buzan, E., Safner, T., Bego, F., Gačić, D., Maletić, V., Markov, G., Milošević, D., Papaioannou, H.,& Sprem, N.. (2022). The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA. in Conservation Genetics, 23(3), 527-539.
https://doi.org/10.1007/s10592-022-01434-w
conv_1616

Rezić A, Iacolina L, Buzan E, Safner T, Bego F, Gačić D, Maletić V, Markov G, Milošević D, Papaioannou H, Sprem N. The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA. in Conservation Genetics. 2022;23(3):527-539.
doi:10.1007/s10592-022-01434-w
conv_1616 .

Rezić, Andrea, Iacolina, Laura, Buzan, Elena, Safner, Toni, Bego, Ferdinand, Gačić, Dragan, Maletić, Vladimir, Markov, Georgi, Milošević, Dragana, Papaioannou, Haritakis, Sprem, Nikica, "The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA" in Conservation Genetics, 23, no. 3 (2022):527-539,
https://doi.org/10.1007/s10592-022-01434-w .,
conv_1616 .