TY  - JOUR
AU  - Tkaczyk, Milosz
AU  - Sikora, Katarzyna
AU  - Galko, Juraj
AU  - Kunca, Andrej
AU  - Milenković, Ivan
PY  - 2020
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1120
AB  - During the monitoring of oak decline phenomenon in Slovakia, symptoms indicative ofPhytophthoradiseases were observed in sessile oak stands in western Slovakia. The study aimed to test the presence and diversity ofPhytophthoraspecies associated with declining oak stands. From rhizosphere soil samples,Phytophthora plurivora,P. quercinaandPythium intermediumwere detected. Soil inoculation tests withP. plurivoraandP. quercinaon 1-year-old oak plants inflicted damage to the root system, and significant differences in measured root parameters between the inoculated and control treatments after 5 months. Although more wilting symptoms were observed inP. plurivora-infected seedlings (7 out of 12 inoculated seedlings),P. quercinacaused the highest amount of damage to root systems, and for example, fine root length was 1.6 times shorter compared to the control. To our knowledge, this is the first report ofP. plurivoraandP. quercinain sessile oak stands in Slovakia.
T2  - Forest Pathology
T1  - Isolation and pathogenicity of Phytophthora species from sessile oak ( Quercus petraea (Matt.) Liebl.) stands in Slovakia
IS  - 5
VL  - 50
DO  - 10.1111/efp.12632
UR  - conv_930
ER  - 

@article{
author = "Tkaczyk, Milosz and Sikora, Katarzyna and Galko, Juraj and Kunca, Andrej and Milenković, Ivan",
year = "2020",
abstract = "During the monitoring of oak decline phenomenon in Slovakia, symptoms indicative ofPhytophthoradiseases were observed in sessile oak stands in western Slovakia. The study aimed to test the presence and diversity ofPhytophthoraspecies associated with declining oak stands. From rhizosphere soil samples,Phytophthora plurivora,P. quercinaandPythium intermediumwere detected. Soil inoculation tests withP. plurivoraandP. quercinaon 1-year-old oak plants inflicted damage to the root system, and significant differences in measured root parameters between the inoculated and control treatments after 5 months. Although more wilting symptoms were observed inP. plurivora-infected seedlings (7 out of 12 inoculated seedlings),P. quercinacaused the highest amount of damage to root systems, and for example, fine root length was 1.6 times shorter compared to the control. To our knowledge, this is the first report ofP. plurivoraandP. quercinain sessile oak stands in Slovakia.",
journal = "Forest Pathology",
title = "Isolation and pathogenicity of Phytophthora species from sessile oak ( Quercus petraea (Matt.) Liebl.) stands in Slovakia",
number = "5",
volume = "50",
doi = "10.1111/efp.12632",
url = "conv_930"
}

Tkaczyk, M., Sikora, K., Galko, J., Kunca, A.,& Milenković, I.. (2020). Isolation and pathogenicity of Phytophthora species from sessile oak ( Quercus petraea (Matt.) Liebl.) stands in Slovakia. in Forest Pathology, 50(5).
https://doi.org/10.1111/efp.12632
conv_930

Tkaczyk M, Sikora K, Galko J, Kunca A, Milenković I. Isolation and pathogenicity of Phytophthora species from sessile oak ( Quercus petraea (Matt.) Liebl.) stands in Slovakia. in Forest Pathology. 2020;50(5).
doi:10.1111/efp.12632
conv_930 .

Tkaczyk, Milosz, Sikora, Katarzyna, Galko, Juraj, Kunca, Andrej, Milenković, Ivan, "Isolation and pathogenicity of Phytophthora species from sessile oak ( Quercus petraea (Matt.) Liebl.) stands in Slovakia" in Forest Pathology, 50, no. 5 (2020),
https://doi.org/10.1111/efp.12632 .,
conv_930 .

TY  - JOUR
AU  - Milenković, Ivan
AU  - Keča, Nenad
AU  - Karadžić, Dragan
AU  - Nowakowska, Justyna A.
AU  - Oszako, Tomasz
AU  - Sikora, Katarzyna
AU  - Tkaczyk, Milosz
PY  - 2018
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/911
AB  - Common pathogenicity tests on 16-month-old ash plants were performed using isolates of Hymenoscyphus fraxineus and three different Phytophthora spp. isolated under declining ash trees in Wolica Nature reserve in Poland. There were no statistically significant differences among the different inoculation treatments and lesion lengths averaged 1.67 cm. After analyses of inoculated roots, five out of 10 root parameters showed statistically significant differences, and after a Dun can's Multiple Range test, significant differences were found with control plants while there were no differences among the treatments. The largest root loss was with the treatment inoculated with both H fraxineus and P. plurivora (HF + PP), and both fine and total root lengths were 2.30 and 2.27 times smaller, respectively, than the non-inoculated control. The earliest mortality of young ash plants was observed also with the I IF + PP treatment. Results showed high aggressiveness of IL fraxineus to young ash plants, with indications that root infections with Phytophthora spp. may contribute to greater damage, but more research is needed to clarify the interaction between H. fraxineus and Phytophthora spp. in ash dieback.
T2  - Forestry Chronicle
T1  - Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings
EP  - 139
IS  - 2
SP  - 135
VL  - 94
DO  - 10.5558/tfc2018-020
UR  - conv_1338
ER  - 

@article{
author = "Milenković, Ivan and Keča, Nenad and Karadžić, Dragan and Nowakowska, Justyna A. and Oszako, Tomasz and Sikora, Katarzyna and Tkaczyk, Milosz",
year = "2018",
abstract = "Common pathogenicity tests on 16-month-old ash plants were performed using isolates of Hymenoscyphus fraxineus and three different Phytophthora spp. isolated under declining ash trees in Wolica Nature reserve in Poland. There were no statistically significant differences among the different inoculation treatments and lesion lengths averaged 1.67 cm. After analyses of inoculated roots, five out of 10 root parameters showed statistically significant differences, and after a Dun can's Multiple Range test, significant differences were found with control plants while there were no differences among the treatments. The largest root loss was with the treatment inoculated with both H fraxineus and P. plurivora (HF + PP), and both fine and total root lengths were 2.30 and 2.27 times smaller, respectively, than the non-inoculated control. The earliest mortality of young ash plants was observed also with the I IF + PP treatment. Results showed high aggressiveness of IL fraxineus to young ash plants, with indications that root infections with Phytophthora spp. may contribute to greater damage, but more research is needed to clarify the interaction between H. fraxineus and Phytophthora spp. in ash dieback.",
journal = "Forestry Chronicle",
title = "Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings",
pages = "139-135",
number = "2",
volume = "94",
doi = "10.5558/tfc2018-020",
url = "conv_1338"
}

Milenković, I., Keča, N., Karadžić, D., Nowakowska, J. A., Oszako, T., Sikora, K.,& Tkaczyk, M.. (2018). Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings. in Forestry Chronicle, 94(2), 135-139.
https://doi.org/10.5558/tfc2018-020
conv_1338

Milenković I, Keča N, Karadžić D, Nowakowska JA, Oszako T, Sikora K, Tkaczyk M. Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings. in Forestry Chronicle. 2018;94(2):135-139.
doi:10.5558/tfc2018-020
conv_1338 .

Milenković, Ivan, Keča, Nenad, Karadžić, Dragan, Nowakowska, Justyna A., Oszako, Tomasz, Sikora, Katarzyna, Tkaczyk, Milosz, "Interaction between Hymenoscyphus fraxineus and Phytophthora species on young Fraxinus excelsior seedlings" in Forestry Chronicle, 94, no. 2 (2018):135-139,
https://doi.org/10.5558/tfc2018-020 .,
conv_1338 .

TY  - JOUR
AU  - Tkaczyk, Milosz
AU  - Milenković, Ivan
AU  - Nowakowska, Justyna A.
AU  - Borys, Malgorzata
AU  - Kaluski, Tomasz
AU  - Gawlak, Magdalena
AU  - Czyz, Michal
AU  - Oszako, Tomasz
PY  - 2017
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/806
AB  - The following paper presents the results on the determination of the diversity of species from the Phytophthora genus occurring in the declining oak stands in Krotoszyn Plateau in Poland. From the 50s of the last century, significant deterioration of oak health was observed in these stands, and Phytophthora species were suggested as one of the factors of the decline. In order to determine the presence of pathogenic organisms from the Phytophthora genus in these stands, 180 rhizosphere soil samples from three forest districts throughout the Krotoszyn Plateau were collected and subjected to the isolation method. Phytophthora species were consistently isolated from all the sampled stands, and 194 isolates from 111 positive samples were obtained. However, 150 (77%) and 44 (23%) isolates originated from the samples taken under the symptomatic and asymptomatic trees, respectively. All the obtained isolates were morphologically classified using the light and scanning electron microscopy and divided into morphological groups. Genomic DNA was isolated from selected isolates representing each group, ITS regions were amplified and sequence analyses were performed. In total, four different Phytophthora species were detected, including P. cactorum, P. plurivora, P. quercina and P. europaea. The most often isolated species were P. cactorum and P. plurivora. This is the first report of P. europaea in oak stands in Poland.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau
EP  - 215
IS  - 1
SP  - 203
VL  - 49
DO  - 10.2298/GENSR1701203T
UR  - conv_1279
ER  - 

@article{
author = "Tkaczyk, Milosz and Milenković, Ivan and Nowakowska, Justyna A. and Borys, Malgorzata and Kaluski, Tomasz and Gawlak, Magdalena and Czyz, Michal and Oszako, Tomasz",
year = "2017",
abstract = "The following paper presents the results on the determination of the diversity of species from the Phytophthora genus occurring in the declining oak stands in Krotoszyn Plateau in Poland. From the 50s of the last century, significant deterioration of oak health was observed in these stands, and Phytophthora species were suggested as one of the factors of the decline. In order to determine the presence of pathogenic organisms from the Phytophthora genus in these stands, 180 rhizosphere soil samples from three forest districts throughout the Krotoszyn Plateau were collected and subjected to the isolation method. Phytophthora species were consistently isolated from all the sampled stands, and 194 isolates from 111 positive samples were obtained. However, 150 (77%) and 44 (23%) isolates originated from the samples taken under the symptomatic and asymptomatic trees, respectively. All the obtained isolates were morphologically classified using the light and scanning electron microscopy and divided into morphological groups. Genomic DNA was isolated from selected isolates representing each group, ITS regions were amplified and sequence analyses were performed. In total, four different Phytophthora species were detected, including P. cactorum, P. plurivora, P. quercina and P. europaea. The most often isolated species were P. cactorum and P. plurivora. This is the first report of P. europaea in oak stands in Poland.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau",
pages = "215-203",
number = "1",
volume = "49",
doi = "10.2298/GENSR1701203T",
url = "conv_1279"
}

Tkaczyk, M., Milenković, I., Nowakowska, J. A., Borys, M., Kaluski, T., Gawlak, M., Czyz, M.,& Oszako, T.. (2017). Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 49(1), 203-215.
https://doi.org/10.2298/GENSR1701203T
conv_1279

Tkaczyk M, Milenković I, Nowakowska JA, Borys M, Kaluski T, Gawlak M, Czyz M, Oszako T. Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau. in Genetika-Belgrade. 2017;49(1):203-215.
doi:10.2298/GENSR1701203T
conv_1279 .

Tkaczyk, Milosz, Milenković, Ivan, Nowakowska, Justyna A., Borys, Malgorzata, Kaluski, Tomasz, Gawlak, Magdalena, Czyz, Michal, Oszako, Tomasz, "Morphological and molecular identification of phytophthora species isolated from the rhizosphere of declining oak trees in krotoszyn plateau" in Genetika-Belgrade, 49, no. 1 (2017):203-215,
https://doi.org/10.2298/GENSR1701203T .,
conv_1279 .

TY  - JOUR
AU  - Pacia, Artur
AU  - Nowakowska, Justyna A.
AU  - Tkaczyk, Milosz
AU  - Sikora, Katarzyna
AU  - Tereba, Anna
AU  - Borys, Malgorzata
AU  - Milenković, Ivan
AU  - Pszczolkowska, Agnieszka
AU  - Okorski, Adam
AU  - Oszako, Tomasz
PY  - 2017
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/833
AB  - The ash stand in Wolica reserve (Poland), affected with ash dieback, was studied. Isolations performed from collected ash tissues and rhizosphere soil samples revealed 28 isolates of Hymenoscyphus fraxineus and 27 isolates of Phytophthora spp., respectively. The vitality and defoliation of 198 and 176 trees were studied, respectively in 2012 and 2013. In 2013 only one tree was completely vital, while 83 trees were within the degeneration phase. These results suggested that trees being classified in this class are the most vital and the natural genetic resistance should be sought among this vitality class in the future. In total, 112 trees were classified to the stage 2 of vitality, for which large deformation of shoots is typical. Further, monitoring of defoliation in 2013 revealed that the largest number of trees in the stand (126) were moderately damaged trees (defoliation 26-60%), while 47 trees had over 60% of defoliation. The synthetic damage index was 1.58 in 2012 and 1.66 in 2013 indicating that advanced disease processes are occurring in this stand. In addition, sampling, isolation, morphological and molecular identifications of Phytophthora species were performed. After the isolation tests, P. megasperma, P. sp. hungarica, and P. plurivora were obtained. These results were confirmed after the PCR and ITS sequencing. This is the first report of P. sp. hungarica and P. megasperma in the stands of common ash in Poland. The natural genetic variation of the Fraxinus excelsior genome was studied to improve understanding of its role in the adaptation and tolerance processes facing ash dieback phenomenon. Six nuclear microsatellite markers and four chloroplast microsatellite markers have been used in order to assess the genetic diversity of Fraxinus excelsior stand in Poland, categorized into three different Roloff' classes of vitality 0+1, 2 and 3. We demonstrated lack of correlation between three different vitality classes of ash trees and their nuclear or chloroplast genetic differentiation. Nevertheless, the observed heterozygosity (H-O) value was significantly different between vitality classes 2 and 3 assessed with nuclear SSR markers (p = 0.000183 in HSD Tukey test, p  lt  0.05). Also private (Ap) alleles distribution of chloroplast SSR markers significantly differ (p = 0.000 in HSD Tukey test, p  lt  0.05) between the vitality classes 0+1 and 3 of ash trees. Those data suggest that DNA differentiation of F. excelsior at local spatial scale may be driven by gene based tolerance.
PB  - Lietuvos Misku Institutas
T2  - Baltic Forestry
T1  - Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland
EP  - 197
IS  - 1
SP  - 183
VL  - 23
UR  - conv_2043
ER  - 

@article{
author = "Pacia, Artur and Nowakowska, Justyna A. and Tkaczyk, Milosz and Sikora, Katarzyna and Tereba, Anna and Borys, Malgorzata and Milenković, Ivan and Pszczolkowska, Agnieszka and Okorski, Adam and Oszako, Tomasz",
year = "2017",
abstract = "The ash stand in Wolica reserve (Poland), affected with ash dieback, was studied. Isolations performed from collected ash tissues and rhizosphere soil samples revealed 28 isolates of Hymenoscyphus fraxineus and 27 isolates of Phytophthora spp., respectively. The vitality and defoliation of 198 and 176 trees were studied, respectively in 2012 and 2013. In 2013 only one tree was completely vital, while 83 trees were within the degeneration phase. These results suggested that trees being classified in this class are the most vital and the natural genetic resistance should be sought among this vitality class in the future. In total, 112 trees were classified to the stage 2 of vitality, for which large deformation of shoots is typical. Further, monitoring of defoliation in 2013 revealed that the largest number of trees in the stand (126) were moderately damaged trees (defoliation 26-60%), while 47 trees had over 60% of defoliation. The synthetic damage index was 1.58 in 2012 and 1.66 in 2013 indicating that advanced disease processes are occurring in this stand. In addition, sampling, isolation, morphological and molecular identifications of Phytophthora species were performed. After the isolation tests, P. megasperma, P. sp. hungarica, and P. plurivora were obtained. These results were confirmed after the PCR and ITS sequencing. This is the first report of P. sp. hungarica and P. megasperma in the stands of common ash in Poland. The natural genetic variation of the Fraxinus excelsior genome was studied to improve understanding of its role in the adaptation and tolerance processes facing ash dieback phenomenon. Six nuclear microsatellite markers and four chloroplast microsatellite markers have been used in order to assess the genetic diversity of Fraxinus excelsior stand in Poland, categorized into three different Roloff' classes of vitality 0+1, 2 and 3. We demonstrated lack of correlation between three different vitality classes of ash trees and their nuclear or chloroplast genetic differentiation. Nevertheless, the observed heterozygosity (H-O) value was significantly different between vitality classes 2 and 3 assessed with nuclear SSR markers (p = 0.000183 in HSD Tukey test, p  lt  0.05). Also private (Ap) alleles distribution of chloroplast SSR markers significantly differ (p = 0.000 in HSD Tukey test, p  lt  0.05) between the vitality classes 0+1 and 3 of ash trees. Those data suggest that DNA differentiation of F. excelsior at local spatial scale may be driven by gene based tolerance.",
publisher = "Lietuvos Misku Institutas",
journal = "Baltic Forestry",
title = "Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland",
pages = "197-183",
number = "1",
volume = "23",
url = "conv_2043"
}

Pacia, A., Nowakowska, J. A., Tkaczyk, M., Sikora, K., Tereba, A., Borys, M., Milenković, I., Pszczolkowska, A., Okorski, A.,& Oszako, T.. (2017). Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland. in Baltic Forestry
Lietuvos Misku Institutas., 23(1), 183-197.
conv_2043

Pacia A, Nowakowska JA, Tkaczyk M, Sikora K, Tereba A, Borys M, Milenković I, Pszczolkowska A, Okorski A, Oszako T. Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland. in Baltic Forestry. 2017;23(1):183-197.
conv_2043 .

Pacia, Artur, Nowakowska, Justyna A., Tkaczyk, Milosz, Sikora, Katarzyna, Tereba, Anna, Borys, Malgorzata, Milenković, Ivan, Pszczolkowska, Agnieszka, Okorski, Adam, Oszako, Tomasz, "Common Ash Stand Affected by Ash Dieback in the Wolica Nature Reserve in Poland" in Baltic Forestry, 23, no. 1 (2017):183-197,
conv_2043 .

TY  - JOUR
AU  - Tkaczyk, Milosz
AU  - Sikora, Katarzyna
AU  - Nowakowska, Justyna A.
AU  - Aniśko, E.
AU  - Oszako, Tomasz
AU  - Belbahri, L.
AU  - Milenković, Ivan
PY  - 2016
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/785
AB  - To investigate susceptibility of young Scots pine seedlings to four Phytophthora species: Phytophthora cactorum, Phytophthora cambivora, Phytophthora plurivora and Phytophthora pini; seven-day-old seedlings of Scots pine (15 seedlings per experiment) were infected using agar plugs of the respective species. Control group also consisted of 15 seedlings and was inoculated with sterile agar plugs. Results unambiguously show that after 4.5 days, all seedlings show clear signs of infection and display severe symptoms of tissue damage and necrosis. Moreover, three and two seedlings in the P. cactorum and P. cambivora infected seedlings groups, respectively, collapsed. The length of largest necrosis measured 13.4±3.90 mm and was caused by P. cactorum. To rule out any putative contamination or infection by secondary pathogens, re-isolations of pathogens from infection sites were performed and were positive in 100% of plated pieces of infected seedlings. All re-isolations were, however, negative in the case of the control group. Detailed microscopic analyses of infected tissues of young seedlings confirmed the presence of numerous Phytophthora species inside and on the surface of infected seedlings. Therefore, our results suggest Phytophthora spp. and mainly P. cactorum and P. cambivora as aggressive pathogens of Scots pine seedlings and highlight a putative involvement of these species in the damping off of young Scots pine seedlings frequently observed in forest nurseries.
PB  - Forest Research Institute
T2  - Folia Forestalia Polonica, Series A
T1  - Four different Phytophthora species that are able to infect Scots pine seedlings in laboratory conditions
EP  - 130
IS  - 3
SP  - 123
VL  - 58
DO  - 10.1515/ffp-2016-0014
UR  - conv_2129
ER  - 

@article{
author = "Tkaczyk, Milosz and Sikora, Katarzyna and Nowakowska, Justyna A. and Aniśko, E. and Oszako, Tomasz and Belbahri, L. and Milenković, Ivan",
year = "2016",
abstract = "To investigate susceptibility of young Scots pine seedlings to four Phytophthora species: Phytophthora cactorum, Phytophthora cambivora, Phytophthora plurivora and Phytophthora pini; seven-day-old seedlings of Scots pine (15 seedlings per experiment) were infected using agar plugs of the respective species. Control group also consisted of 15 seedlings and was inoculated with sterile agar plugs. Results unambiguously show that after 4.5 days, all seedlings show clear signs of infection and display severe symptoms of tissue damage and necrosis. Moreover, three and two seedlings in the P. cactorum and P. cambivora infected seedlings groups, respectively, collapsed. The length of largest necrosis measured 13.4±3.90 mm and was caused by P. cactorum. To rule out any putative contamination or infection by secondary pathogens, re-isolations of pathogens from infection sites were performed and were positive in 100% of plated pieces of infected seedlings. All re-isolations were, however, negative in the case of the control group. Detailed microscopic analyses of infected tissues of young seedlings confirmed the presence of numerous Phytophthora species inside and on the surface of infected seedlings. Therefore, our results suggest Phytophthora spp. and mainly P. cactorum and P. cambivora as aggressive pathogens of Scots pine seedlings and highlight a putative involvement of these species in the damping off of young Scots pine seedlings frequently observed in forest nurseries.",
publisher = "Forest Research Institute",
journal = "Folia Forestalia Polonica, Series A",
title = "Four different Phytophthora species that are able to infect Scots pine seedlings in laboratory conditions",
pages = "130-123",
number = "3",
volume = "58",
doi = "10.1515/ffp-2016-0014",
url = "conv_2129"
}

Tkaczyk, M., Sikora, K., Nowakowska, J. A., Aniśko, E., Oszako, T., Belbahri, L.,& Milenković, I.. (2016). Four different Phytophthora species that are able to infect Scots pine seedlings in laboratory conditions. in Folia Forestalia Polonica, Series A
Forest Research Institute., 58(3), 123-130.
https://doi.org/10.1515/ffp-2016-0014
conv_2129

Tkaczyk M, Sikora K, Nowakowska JA, Aniśko E, Oszako T, Belbahri L, Milenković I. Four different Phytophthora species that are able to infect Scots pine seedlings in laboratory conditions. in Folia Forestalia Polonica, Series A. 2016;58(3):123-130.
doi:10.1515/ffp-2016-0014
conv_2129 .

Tkaczyk, Milosz, Sikora, Katarzyna, Nowakowska, Justyna A., Aniśko, E., Oszako, Tomasz, Belbahri, L., Milenković, Ivan, "Four different Phytophthora species that are able to infect Scots pine seedlings in laboratory conditions" in Folia Forestalia Polonica, Series A, 58, no. 3 (2016):123-130,
https://doi.org/10.1515/ffp-2016-0014 .,
conv_2129 .