TY  - JOUR
AU  - Skočajić, Dragana
AU  - Gasić, Uroš
AU  - Dabic Zagorac, Dragana
AU  - Nešić, Marija
AU  - Tesić, Zivoslav
AU  - Meland, Mekjell
AU  - Fotiric Aksić, Milica
PY  - 2021
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1192
AB  - The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars ('Amanogawa', 'Kanzan', and 'Kiku-shidare-zakura') and commercial rootstocks. Prunus avium, Prunus 'Colt', Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis 'Pyrodwarf' were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with 'Amanogawa' and 'Kiku-shidare-zakura' the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.
T2  - Plants-Basel
T1  - Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries
IS  - 12
VL  - 10
DO  - 10.3390/plants10122822
UR  - conv_1607
ER  - 

@article{
author = "Skočajić, Dragana and Gasić, Uroš and Dabic Zagorac, Dragana and Nešić, Marija and Tesić, Zivoslav and Meland, Mekjell and Fotiric Aksić, Milica",
year = "2021",
abstract = "The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars ('Amanogawa', 'Kanzan', and 'Kiku-shidare-zakura') and commercial rootstocks. Prunus avium, Prunus 'Colt', Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis 'Pyrodwarf' were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with 'Amanogawa' and 'Kiku-shidare-zakura' the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.",
journal = "Plants-Basel",
title = "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries",
number = "12",
volume = "10",
doi = "10.3390/plants10122822",
url = "conv_1607"
}

Skočajić, D., Gasić, U., Dabic Zagorac, D., Nešić, M., Tesić, Z., Meland, M.,& Fotiric Aksić, M.. (2021). Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries. in Plants-Basel, 10(12).
https://doi.org/10.3390/plants10122822
conv_1607

Skočajić D, Gasić U, Dabic Zagorac D, Nešić M, Tesić Z, Meland M, Fotiric Aksić M. Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries. in Plants-Basel. 2021;10(12).
doi:10.3390/plants10122822
conv_1607 .

Skočajić, Dragana, Gasić, Uroš, Dabic Zagorac, Dragana, Nešić, Marija, Tesić, Zivoslav, Meland, Mekjell, Fotiric Aksić, Milica, "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries" in Plants-Basel, 10, no. 12 (2021),
https://doi.org/10.3390/plants10122822 .,
conv_1607 .

TY  - JOUR
AU  - Skočajić, Dragana
AU  - Nešić, Marija
AU  - Nonić, Marina
AU  - Fotiric Aksić, Milica M.
AU  - Grbić, Mihailo
AU  - Dukić, Matilda S.
AU  - Šijačić-Nikolić, Mirjana
PY  - 2017
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/862
AB  - Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.
T2  - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
T1  - In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks
EP  - 399
IS  - 2
SP  - 392
VL  - 45
DO  - 10.15835/nbha45210899
UR  - conv_1301
ER  - 

@article{
author = "Skočajić, Dragana and Nešić, Marija and Nonić, Marina and Fotiric Aksić, Milica M. and Grbić, Mihailo and Dukić, Matilda S. and Šijačić-Nikolić, Mirjana",
year = "2017",
abstract = "Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.",
journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca",
title = "In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks",
pages = "399-392",
number = "2",
volume = "45",
doi = "10.15835/nbha45210899",
url = "conv_1301"
}

Skočajić, D., Nešić, M., Nonić, M., Fotiric Aksić, M. M., Grbić, M., Dukić, M. S.,& Šijačić-Nikolić, M.. (2017). In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(2), 392-399.
https://doi.org/10.15835/nbha45210899
conv_1301

Skočajić D, Nešić M, Nonić M, Fotiric Aksić MM, Grbić M, Dukić MS, Šijačić-Nikolić M. In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2017;45(2):392-399.
doi:10.15835/nbha45210899
conv_1301 .

Skočajić, Dragana, Nešić, Marija, Nonić, Marina, Fotiric Aksić, Milica M., Grbić, Mihailo, Dukić, Matilda S., Šijačić-Nikolić, Mirjana, "In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45, no. 2 (2017):392-399,
https://doi.org/10.15835/nbha45210899 .,
conv_1301 .