TY  - JOUR
AU  - Skočajić, Dragana
AU  - Nešić, Marija
AU  - Nonić, Marina
AU  - Fotiric Aksić, Milica M.
AU  - Grbić, Mihailo
AU  - Dukić, Matilda S.
AU  - Šijačić-Nikolić, Mirjana
PY  - 2017
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/862
AB  - Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.
T2  - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
T1  - In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks
EP  - 399
IS  - 2
SP  - 392
VL  - 45
DO  - 10.15835/nbha45210899
UR  - conv_1301
ER  - 

@article{
author = "Skočajić, Dragana and Nešić, Marija and Nonić, Marina and Fotiric Aksić, Milica M. and Grbić, Mihailo and Dukić, Matilda S. and Šijačić-Nikolić, Mirjana",
year = "2017",
abstract = "Several in vitro biotechnological techniques have been developed, all of which require a reliable protocol to produce a responsive callus mass. One of these techniques is callus fusion in vitro, which is reliable for the early detection of (in)compatibility of scions and rootstocks. In this paper, the possibility to obtain friable callus tissues was explored by callus induction of adult tissues of Japanese flowering cherry trees from the group Sato zakura (Prunus serrulata 'Amanogawa', 'Kanzan' and 'Kiku-shidare-zakura') and two domestic cherry rootstocks -Prunus avium and Prunus 'Colt'. The explants used in the research were: leaf petiole, leaf base with a part of a petiole, part of lamina with a midvein and a stem with an axillary bud. Among three plant growth media (MS, SH and WP) that were used in this study, the MS proved to be the most favourable for the majority of taxa during the callus induction process. For the sweet cherry tree and the cultivars 'Kanzan' and 'Colt', the SH plant growth medium was also acceptable. The best results in callogenesis were obtained for the majority of taxons with auxin at the concentration 2 mgL-1 NAA and cytokinin BAP 0.5 mgL-1. It is also possible to use 2.4-D at the same concentration as a substitute for the genotypes Prunus avium, Prunus ` Colt' and Prunus serrulata 'Kanzan', whereas IBA proved to be an inappropriate auxin for callus induction. The protocol described herein is proved to be efficient callus induction in a range of taxa of genus Prunus.",
journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca",
title = "In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks",
pages = "399-392",
number = "2",
volume = "45",
doi = "10.15835/nbha45210899",
url = "conv_1301"
}

Skočajić, D., Nešić, M., Nonić, M., Fotiric Aksić, M. M., Grbić, M., Dukić, M. S.,& Šijačić-Nikolić, M.. (2017). In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45(2), 392-399.
https://doi.org/10.15835/nbha45210899
conv_1301

Skočajić D, Nešić M, Nonić M, Fotiric Aksić MM, Grbić M, Dukić MS, Šijačić-Nikolić M. In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2017;45(2):392-399.
doi:10.15835/nbha45210899
conv_1301 .

Skočajić, Dragana, Nešić, Marija, Nonić, Marina, Fotiric Aksić, Milica M., Grbić, Mihailo, Dukić, Matilda S., Šijačić-Nikolić, Mirjana, "In Vitro Callus Induction from Adult Tissues of Japanese Flowering Cherry Trees and Two Cherry Rootstocks" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 45, no. 2 (2017):392-399,
https://doi.org/10.15835/nbha45210899 .,
conv_1301 .