TY  - JOUR
AU  - Puertolas, Jaime
AU  - Villar-Salvador, Pedro
AU  - Andivia, Enrique
AU  - Ahuja, Ishita
AU  - Cocozza, Claudia
AU  - Cvjetković, Branislav
AU  - Devetaković, Jovana
AU  - Diez, Julio J.
AU  - Floistad, Inger S.
AU  - Ganatsas, Petros
AU  - Mariotti, Barbara
AU  - Tsakaldimi, Marianthi
AU  - Vilagrosa, Alberto
AU  - Witzell, Johanna
AU  - Ivetić, Vladan
PY  - 2024
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1507
AB  - Drought hardening is a nursery technique aimed to enhance early forest plantation establishment under dry conditions, which is a main limiting factors for plantation success. However, the quantitative effectiveness of drought hardening remains unclear. We conducted a meta-analysis to evaluate the influence of different factors in the effectiveness of drought hardening on seedling post-planting survival and growth. Overall, drought hardening did not significantly affect survival or growth, as several factors induced great heterogeneity, but analyses of those factors explained its effectiveness, especially on survival. A longer time between hardening and transplanting strongly reduced survival. Indoor-grown seedlings did not benefit more from hardening than outdoor-grown seedlings. Evaluations of drought hardening effectiveness in pots showed positive effects on survival but negative effects on growth, while no effects were found in large bed experiments. In field experiments, hardening significantly increased survival and growth with site aridity. Survival benefits were independent of species drought tolerance, measured by osmotic potential at the turgor loss point (pi tlp), in moderate to high aridity sites. However, in low aridity sites, hardening increased survival in drought-tolerant species but decreased it in drought-intolerant species. Field results showed that hardening benefited shrubs more than trees in angiosperms. In conclusion, drought hardening at the end of nursery cultivation tend to increase post-planting seedling performance particularly in scenarios limiting post-planting root growth such as in arid climates and pot experiments. Our findings highlight the importance of future research on modelling the interaction between these technical features and species water use strategies..
T2  - Forest Ecology and Management
T1  - Die-hard seedlings. A global meta-analysis on the factors determining the effectiveness of drought hardening on growth and survival of forest plantations
VL  - 572
DO  - 10.1016/j.foreco.2024.122300
UR  - conv_1826
ER  - 

@article{
author = "Puertolas, Jaime and Villar-Salvador, Pedro and Andivia, Enrique and Ahuja, Ishita and Cocozza, Claudia and Cvjetković, Branislav and Devetaković, Jovana and Diez, Julio J. and Floistad, Inger S. and Ganatsas, Petros and Mariotti, Barbara and Tsakaldimi, Marianthi and Vilagrosa, Alberto and Witzell, Johanna and Ivetić, Vladan",
year = "2024",
abstract = "Drought hardening is a nursery technique aimed to enhance early forest plantation establishment under dry conditions, which is a main limiting factors for plantation success. However, the quantitative effectiveness of drought hardening remains unclear. We conducted a meta-analysis to evaluate the influence of different factors in the effectiveness of drought hardening on seedling post-planting survival and growth. Overall, drought hardening did not significantly affect survival or growth, as several factors induced great heterogeneity, but analyses of those factors explained its effectiveness, especially on survival. A longer time between hardening and transplanting strongly reduced survival. Indoor-grown seedlings did not benefit more from hardening than outdoor-grown seedlings. Evaluations of drought hardening effectiveness in pots showed positive effects on survival but negative effects on growth, while no effects were found in large bed experiments. In field experiments, hardening significantly increased survival and growth with site aridity. Survival benefits were independent of species drought tolerance, measured by osmotic potential at the turgor loss point (pi tlp), in moderate to high aridity sites. However, in low aridity sites, hardening increased survival in drought-tolerant species but decreased it in drought-intolerant species. Field results showed that hardening benefited shrubs more than trees in angiosperms. In conclusion, drought hardening at the end of nursery cultivation tend to increase post-planting seedling performance particularly in scenarios limiting post-planting root growth such as in arid climates and pot experiments. Our findings highlight the importance of future research on modelling the interaction between these technical features and species water use strategies..",
journal = "Forest Ecology and Management",
title = "Die-hard seedlings. A global meta-analysis on the factors determining the effectiveness of drought hardening on growth and survival of forest plantations",
volume = "572",
doi = "10.1016/j.foreco.2024.122300",
url = "conv_1826"
}

Puertolas, J., Villar-Salvador, P., Andivia, E., Ahuja, I., Cocozza, C., Cvjetković, B., Devetaković, J., Diez, J. J., Floistad, I. S., Ganatsas, P., Mariotti, B., Tsakaldimi, M., Vilagrosa, A., Witzell, J.,& Ivetić, V.. (2024). Die-hard seedlings. A global meta-analysis on the factors determining the effectiveness of drought hardening on growth and survival of forest plantations. in Forest Ecology and Management, 572.
https://doi.org/10.1016/j.foreco.2024.122300
conv_1826

Puertolas J, Villar-Salvador P, Andivia E, Ahuja I, Cocozza C, Cvjetković B, Devetaković J, Diez JJ, Floistad IS, Ganatsas P, Mariotti B, Tsakaldimi M, Vilagrosa A, Witzell J, Ivetić V. Die-hard seedlings. A global meta-analysis on the factors determining the effectiveness of drought hardening on growth and survival of forest plantations. in Forest Ecology and Management. 2024;572.
doi:10.1016/j.foreco.2024.122300
conv_1826 .

Puertolas, Jaime, Villar-Salvador, Pedro, Andivia, Enrique, Ahuja, Ishita, Cocozza, Claudia, Cvjetković, Branislav, Devetaković, Jovana, Diez, Julio J., Floistad, Inger S., Ganatsas, Petros, Mariotti, Barbara, Tsakaldimi, Marianthi, Vilagrosa, Alberto, Witzell, Johanna, Ivetić, Vladan, "Die-hard seedlings. A global meta-analysis on the factors determining the effectiveness of drought hardening on growth and survival of forest plantations" in Forest Ecology and Management, 572 (2024),
https://doi.org/10.1016/j.foreco.2024.122300 .,
conv_1826 .

TY  - JOUR
AU  - Tsakaldimi, Marianthi
AU  - Giannaki, Panagiota
AU  - Ivetić, Vladan
AU  - Kapsali, Nikoleta
AU  - Ganatsas, Petros
PY  - 2021
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1240
AB  - Pinus nigra is one of the most widely used tree species for reforestation within its geographical distribution, as well as being a potential substitute for other tree species in Central Europe under future climate scenarios. P. nigra is transplanted into the field as two-year or three-year old seedlings because of its relatively low growth rate in the nursery. This study investigated the effects of fertilization programs and shading on P. nigra seedlings, aiming to accelerate early growth, and thus to reduce the nursery rearing time. The experiment (a completely randomized block design) was conducted in an open-air nursery by sowing seeds from Grevena, Northern Greece, in Quick pots filled with peat and perlite in a 2:1 ratio. The seedlings were subjected to two levels of fertilization-5 and 10 g L-1 NPK (30-10-10)-and two shading levels: 50% and 70%. At the ends of the first and second nursery growing season, we recorded the seedlings' above- and below-ground morphology and biomass data. The results show that the application of all of the treatments produced seedlings which met the targeted quality standards for outplanting. However, the combination of a high fertilization rate and low shading level resulted in seedlings of a higher morphological quality, which is often considered to be an indicator for a successful seedling establishment in the field.
T2  - Sustainability
T1  - Fertilization and Shading Trials to Promote Pinus nigra Seedlings' Nursery Growth under the Climate Change Demands
IS  - 6
VL  - 13
DO  - 10.3390/su13063563
UR  - conv_1545
ER  - 

@article{
author = "Tsakaldimi, Marianthi and Giannaki, Panagiota and Ivetić, Vladan and Kapsali, Nikoleta and Ganatsas, Petros",
year = "2021",
abstract = "Pinus nigra is one of the most widely used tree species for reforestation within its geographical distribution, as well as being a potential substitute for other tree species in Central Europe under future climate scenarios. P. nigra is transplanted into the field as two-year or three-year old seedlings because of its relatively low growth rate in the nursery. This study investigated the effects of fertilization programs and shading on P. nigra seedlings, aiming to accelerate early growth, and thus to reduce the nursery rearing time. The experiment (a completely randomized block design) was conducted in an open-air nursery by sowing seeds from Grevena, Northern Greece, in Quick pots filled with peat and perlite in a 2:1 ratio. The seedlings were subjected to two levels of fertilization-5 and 10 g L-1 NPK (30-10-10)-and two shading levels: 50% and 70%. At the ends of the first and second nursery growing season, we recorded the seedlings' above- and below-ground morphology and biomass data. The results show that the application of all of the treatments produced seedlings which met the targeted quality standards for outplanting. However, the combination of a high fertilization rate and low shading level resulted in seedlings of a higher morphological quality, which is often considered to be an indicator for a successful seedling establishment in the field.",
journal = "Sustainability",
title = "Fertilization and Shading Trials to Promote Pinus nigra Seedlings' Nursery Growth under the Climate Change Demands",
number = "6",
volume = "13",
doi = "10.3390/su13063563",
url = "conv_1545"
}

Tsakaldimi, M., Giannaki, P., Ivetić, V., Kapsali, N.,& Ganatsas, P.. (2021). Fertilization and Shading Trials to Promote Pinus nigra Seedlings' Nursery Growth under the Climate Change Demands. in Sustainability, 13(6).
https://doi.org/10.3390/su13063563
conv_1545

Tsakaldimi M, Giannaki P, Ivetić V, Kapsali N, Ganatsas P. Fertilization and Shading Trials to Promote Pinus nigra Seedlings' Nursery Growth under the Climate Change Demands. in Sustainability. 2021;13(6).
doi:10.3390/su13063563
conv_1545 .

Tsakaldimi, Marianthi, Giannaki, Panagiota, Ivetić, Vladan, Kapsali, Nikoleta, Ganatsas, Petros, "Fertilization and Shading Trials to Promote Pinus nigra Seedlings' Nursery Growth under the Climate Change Demands" in Sustainability, 13, no. 6 (2021),
https://doi.org/10.3390/su13063563 .,
conv_1545 .

TY  - JOUR
AU  - Ivetić, Vladan
AU  - Tsakaldimi, Marianthi
AU  - Ganatsas, Petros
AU  - Kerkez-Janković, Ivona
AU  - Devetaković, Jovana
PY  - 2021
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1174
AB  - To meet the restoration and reforestation goals in the changing environment, the translocation of genotypes and species northward and upward need to be considered to a great extent. Pinus nigra is a genetically diverse, drought sensitive species, with cold hardiness comparable to other tree species under the same climatic conditions. This study tested frost hardiness (whole plant freezing test-WPFT, and electric conductivity-EC test), and heat tolerance (heat tolerance test) of P. nigra seedlings from two southern Greek provenances (Kalamata and Grevena) and one northern Serbian provenance (Sargan) to better understand the potential of seed transfer from the south to the north of the species distribution in the Balkan peninsula. The results showed that, that for all studied provenances, the damage was great; the index of injury (Ii) at -18 degrees C was ranged from 49 to 54.5 (measured by the EC method) and the percentage of injured tissues ranged from 80-90% (measured by visual observation). For all studied provenances, a sharp increase in damages was observed with the fall of temperature from -5 and -18 degrees C and the time after exposure. The WPFT results showed that the highest tolerance to freezing (-18 degrees C) was presented by seedlings from the northern (Sargan) provenance; however, no significant differences were statistically detected among the studied provenances. The heat and drought-treated seedlings, from both provenances, presented significantly highler foliar damages than only drought-treated ones. For seedlings from both contrasting provenances (Grevena and Sargan), exposure to moderate heat (45 degrees C) and short drought did present damages but without significant difference between them. Considering freezing and heating tolerance, Greek provenances of P. nigra (i.e., Grevena region) can be successfully used in Serbian forestation and restoration programs. The present study makes a contribution towards P. nigra reforestation with practical implications for abiotic stress (frost, heat drought) tolerance among southern and northern provenances and could be valuable to determine the suitable provenances for reforestation programs and assisted population migration under climatic change scenarios.
T2  - Sustainability
T1  - Freezing and Heating Tolerance of Pinus nigra Seedlings from Three South to North Balkan Provenances
IS  - 16
VL  - 13
DO  - 10.3390/su13169290
UR  - conv_1571
ER  - 

@article{
author = "Ivetić, Vladan and Tsakaldimi, Marianthi and Ganatsas, Petros and Kerkez-Janković, Ivona and Devetaković, Jovana",
year = "2021",
abstract = "To meet the restoration and reforestation goals in the changing environment, the translocation of genotypes and species northward and upward need to be considered to a great extent. Pinus nigra is a genetically diverse, drought sensitive species, with cold hardiness comparable to other tree species under the same climatic conditions. This study tested frost hardiness (whole plant freezing test-WPFT, and electric conductivity-EC test), and heat tolerance (heat tolerance test) of P. nigra seedlings from two southern Greek provenances (Kalamata and Grevena) and one northern Serbian provenance (Sargan) to better understand the potential of seed transfer from the south to the north of the species distribution in the Balkan peninsula. The results showed that, that for all studied provenances, the damage was great; the index of injury (Ii) at -18 degrees C was ranged from 49 to 54.5 (measured by the EC method) and the percentage of injured tissues ranged from 80-90% (measured by visual observation). For all studied provenances, a sharp increase in damages was observed with the fall of temperature from -5 and -18 degrees C and the time after exposure. The WPFT results showed that the highest tolerance to freezing (-18 degrees C) was presented by seedlings from the northern (Sargan) provenance; however, no significant differences were statistically detected among the studied provenances. The heat and drought-treated seedlings, from both provenances, presented significantly highler foliar damages than only drought-treated ones. For seedlings from both contrasting provenances (Grevena and Sargan), exposure to moderate heat (45 degrees C) and short drought did present damages but without significant difference between them. Considering freezing and heating tolerance, Greek provenances of P. nigra (i.e., Grevena region) can be successfully used in Serbian forestation and restoration programs. The present study makes a contribution towards P. nigra reforestation with practical implications for abiotic stress (frost, heat drought) tolerance among southern and northern provenances and could be valuable to determine the suitable provenances for reforestation programs and assisted population migration under climatic change scenarios.",
journal = "Sustainability",
title = "Freezing and Heating Tolerance of Pinus nigra Seedlings from Three South to North Balkan Provenances",
number = "16",
volume = "13",
doi = "10.3390/su13169290",
url = "conv_1571"
}

Ivetić, V., Tsakaldimi, M., Ganatsas, P., Kerkez-Janković, I.,& Devetaković, J.. (2021). Freezing and Heating Tolerance of Pinus nigra Seedlings from Three South to North Balkan Provenances. in Sustainability, 13(16).
https://doi.org/10.3390/su13169290
conv_1571

Ivetić V, Tsakaldimi M, Ganatsas P, Kerkez-Janković I, Devetaković J. Freezing and Heating Tolerance of Pinus nigra Seedlings from Three South to North Balkan Provenances. in Sustainability. 2021;13(16).
doi:10.3390/su13169290
conv_1571 .

Ivetić, Vladan, Tsakaldimi, Marianthi, Ganatsas, Petros, Kerkez-Janković, Ivona, Devetaković, Jovana, "Freezing and Heating Tolerance of Pinus nigra Seedlings from Three South to North Balkan Provenances" in Sustainability, 13, no. 16 (2021),
https://doi.org/10.3390/su13169290 .,
conv_1571 .