TY  - JOUR
AU  - Stipoljev, Suncica
AU  - Buzan, Elena
AU  - Iacolina, Laura
AU  - Safner, Toni
AU  - Rezić, Andrea
AU  - Galov, Ana
AU  - Krizanović, Kresimir
AU  - Ambarli, Hueseyin
AU  - Arnal, MariaCruz
AU  - Babaev, Elmar
AU  - Bego, Ferdinand
AU  - Farkas, Attila
AU  - Gačić, Dragan
AU  - Lazar, Peter
AU  - Maletić, Vladimir
AU  - Markov, Georgi
AU  - Milošević, Dragana
AU  - Papaioannou, Haritakis
AU  - Scandura, Massimo
AU  - Sprem, Nikica
PY  - 2024
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1498
AB  - Major histocompatibility complex (MHC) genes are commonly used markers for monitoring adaptive genetic and evolutionary potential of species. In this study, we investigated genetic variation of the MHC class II DRB locus in the chamois genus Rupicapra by using next-generation sequencing. Sequencing of 102 samples led to the identification of 25 alleles, 11 of which are novel. The high ratio of the relative rates of nonsynonymous to synonymous mutations (dN/dS) suggests a signal of positive selection on this locus. We analyzed patterns of genetic variation within and among 2 subspecies of Northern Chamois and compared them to previously published studies using neutral markers to provide a basis for assessing the effects of demographic processes. Our analyses have shown that alleles are likely to be maintained by balancing selection in different populations with similar frequencies and that this mechanism also works in small, isolated populations that are strongly affected by genetic drift. In this study on the chamois genus Rupicapra, researchers explored genetic variation of the MHC class II DRB locus using next-generation sequencing. The research highlights a strong signal of positive selection at this locus, indicated by the high dN/dS ratio. The study also revealed that balancing selection maintains alleles across different chamois populations, including those that are small and isolated, emphasizing the evolutionary significance of this genetic mechanism. Graphical Abstract
T2  - Journal of Mammalogy
T1  - Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra
EP  - 322
IS  - 2
SP  - 312
VL  - 105
DO  - 10.1093/jmammal/gyae008
UR  - conv_1761
ER  - 

@article{
author = "Stipoljev, Suncica and Buzan, Elena and Iacolina, Laura and Safner, Toni and Rezić, Andrea and Galov, Ana and Krizanović, Kresimir and Ambarli, Hueseyin and Arnal, MariaCruz and Babaev, Elmar and Bego, Ferdinand and Farkas, Attila and Gačić, Dragan and Lazar, Peter and Maletić, Vladimir and Markov, Georgi and Milošević, Dragana and Papaioannou, Haritakis and Scandura, Massimo and Sprem, Nikica",
year = "2024",
abstract = "Major histocompatibility complex (MHC) genes are commonly used markers for monitoring adaptive genetic and evolutionary potential of species. In this study, we investigated genetic variation of the MHC class II DRB locus in the chamois genus Rupicapra by using next-generation sequencing. Sequencing of 102 samples led to the identification of 25 alleles, 11 of which are novel. The high ratio of the relative rates of nonsynonymous to synonymous mutations (dN/dS) suggests a signal of positive selection on this locus. We analyzed patterns of genetic variation within and among 2 subspecies of Northern Chamois and compared them to previously published studies using neutral markers to provide a basis for assessing the effects of demographic processes. Our analyses have shown that alleles are likely to be maintained by balancing selection in different populations with similar frequencies and that this mechanism also works in small, isolated populations that are strongly affected by genetic drift. In this study on the chamois genus Rupicapra, researchers explored genetic variation of the MHC class II DRB locus using next-generation sequencing. The research highlights a strong signal of positive selection at this locus, indicated by the high dN/dS ratio. The study also revealed that balancing selection maintains alleles across different chamois populations, including those that are small and isolated, emphasizing the evolutionary significance of this genetic mechanism. Graphical Abstract",
journal = "Journal of Mammalogy",
title = "Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra",
pages = "322-312",
number = "2",
volume = "105",
doi = "10.1093/jmammal/gyae008",
url = "conv_1761"
}

Stipoljev, S., Buzan, E., Iacolina, L., Safner, T., Rezić, A., Galov, A., Krizanović, K., Ambarli, H., Arnal, M., Babaev, E., Bego, F., Farkas, A., Gačić, D., Lazar, P., Maletić, V., Markov, G., Milošević, D., Papaioannou, H., Scandura, M.,& Sprem, N.. (2024). Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra. in Journal of Mammalogy, 105(2), 312-322.
https://doi.org/10.1093/jmammal/gyae008
conv_1761

Stipoljev S, Buzan E, Iacolina L, Safner T, Rezić A, Galov A, Krizanović K, Ambarli H, Arnal M, Babaev E, Bego F, Farkas A, Gačić D, Lazar P, Maletić V, Markov G, Milošević D, Papaioannou H, Scandura M, Sprem N. Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra. in Journal of Mammalogy. 2024;105(2):312-322.
doi:10.1093/jmammal/gyae008
conv_1761 .

Stipoljev, Suncica, Buzan, Elena, Iacolina, Laura, Safner, Toni, Rezić, Andrea, Galov, Ana, Krizanović, Kresimir, Ambarli, Hueseyin, Arnal, MariaCruz, Babaev, Elmar, Bego, Ferdinand, Farkas, Attila, Gačić, Dragan, Lazar, Peter, Maletić, Vladimir, Markov, Georgi, Milošević, Dragana, Papaioannou, Haritakis, Scandura, Massimo, Sprem, Nikica, "Diversity of MHC class II DRB alleles in the Northern chamois genus Rupicapra" in Journal of Mammalogy, 105, no. 2 (2024):312-322,
https://doi.org/10.1093/jmammal/gyae008 .,
conv_1761 .

TY  - JOUR
AU  - Corlatti, L.
AU  - Iacolina, L.
AU  - Safner, Toni
AU  - Apollonio, M.
AU  - Buzan, Elena
AU  - Ferretti, F.
AU  - Hammer, S. E.
AU  - Herrero, J.
AU  - Rossi, L.
AU  - Serrano, E.
AU  - Arnal, MariaCruz
AU  - Brivio, F.
AU  - Chirichella, R.
AU  - Cotza, A.
AU  - Crestanello, B.
AU  - Espunyes, J.
AU  - de Luco, D. Fernandez
AU  - Friedrich, S.
AU  - Gačić, Dragan
AU  - Grassi, L.
AU  - Grignolio, S.
AU  - Hauffe, H. C.
AU  - Kavcić, K.
AU  - Kinser, A.
AU  - Lioce, F.
AU  - Malagnino, A.
AU  - Miller, C.
AU  - Peters, W.
AU  - Pokorny, Bostjan
AU  - Reiner, R.
AU  - Rezić, Andrea
AU  - Stipoljev, S.
AU  - Tesija, T.
AU  - Yankov, Y.
AU  - Zwijacz-Kozica, T.
AU  - Sprem, Nikica
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1329
AB  - The chamois Rupicapra spp. is the most abundant mountain ungulate of Europe and the Near East, where it occurs as two species, the northern chamois R. rupicapra and the southern chamois R. pyrenaica. Here, we provide a state-of-the-art overview of research trends and the most challenging issues in chamois research and conservation, focusing on taxonomy and systematics, genetics, life history, ecology and behavior, physiology and disease, management and conservation. Research on Rupicapra has a longstanding history and has contributed substantially to the biological and ecological knowledge of mountain ungulates. Although the number of publications on this genus has markedly increased over the past two decades, major differences persist with respect to knowledge of species and subspecies, with research mostly focusing on the Alpine chamois R. r. rupicapra and, to a lesser extent, the Pyrenean chamois R. p. pyrenaica. In addition, a scarcity of replicate studies of populations of different subspecies and/or geographic areas limits the advancement of chamois science. Since environmental heterogeneity impacts behavioral, physiological and life history traits, understanding the underlying processes would be of great value from both an evolutionary and conservation/management standpoint, especially in the light of ongoing climatic change. Substantial contributions to this challenge may derive from a quantitative assessment of reproductive success, investigation of fine-scale foraging patterns, and a mechanistic understanding of disease outbreak and resilience. For improving conservation status, resolving taxonomic disputes, identifying subspecies hybridization, assessing the impact of hunting and establishing reliable methods of abundance estimation are of primary concern. Despite being one of the most well-known mountain ungulates, substantial field efforts to collect paleontological, behavioral, ecological, morphological, physiological and genetic data on different populations and subspecies are still needed to ensure a successful future for chamois research and conservation.
T2  - Wildlife Biology
T1  - Past, present and future of chamois science
IS  - 4
VL  - 2022
DO  - 10.1002/wlb3.01025
UR  - conv_1628
ER  - 

@article{
author = "Corlatti, L. and Iacolina, L. and Safner, Toni and Apollonio, M. and Buzan, Elena and Ferretti, F. and Hammer, S. E. and Herrero, J. and Rossi, L. and Serrano, E. and Arnal, MariaCruz and Brivio, F. and Chirichella, R. and Cotza, A. and Crestanello, B. and Espunyes, J. and de Luco, D. Fernandez and Friedrich, S. and Gačić, Dragan and Grassi, L. and Grignolio, S. and Hauffe, H. C. and Kavcić, K. and Kinser, A. and Lioce, F. and Malagnino, A. and Miller, C. and Peters, W. and Pokorny, Bostjan and Reiner, R. and Rezić, Andrea and Stipoljev, S. and Tesija, T. and Yankov, Y. and Zwijacz-Kozica, T. and Sprem, Nikica",
year = "2022",
abstract = "The chamois Rupicapra spp. is the most abundant mountain ungulate of Europe and the Near East, where it occurs as two species, the northern chamois R. rupicapra and the southern chamois R. pyrenaica. Here, we provide a state-of-the-art overview of research trends and the most challenging issues in chamois research and conservation, focusing on taxonomy and systematics, genetics, life history, ecology and behavior, physiology and disease, management and conservation. Research on Rupicapra has a longstanding history and has contributed substantially to the biological and ecological knowledge of mountain ungulates. Although the number of publications on this genus has markedly increased over the past two decades, major differences persist with respect to knowledge of species and subspecies, with research mostly focusing on the Alpine chamois R. r. rupicapra and, to a lesser extent, the Pyrenean chamois R. p. pyrenaica. In addition, a scarcity of replicate studies of populations of different subspecies and/or geographic areas limits the advancement of chamois science. Since environmental heterogeneity impacts behavioral, physiological and life history traits, understanding the underlying processes would be of great value from both an evolutionary and conservation/management standpoint, especially in the light of ongoing climatic change. Substantial contributions to this challenge may derive from a quantitative assessment of reproductive success, investigation of fine-scale foraging patterns, and a mechanistic understanding of disease outbreak and resilience. For improving conservation status, resolving taxonomic disputes, identifying subspecies hybridization, assessing the impact of hunting and establishing reliable methods of abundance estimation are of primary concern. Despite being one of the most well-known mountain ungulates, substantial field efforts to collect paleontological, behavioral, ecological, morphological, physiological and genetic data on different populations and subspecies are still needed to ensure a successful future for chamois research and conservation.",
journal = "Wildlife Biology",
title = "Past, present and future of chamois science",
number = "4",
volume = "2022",
doi = "10.1002/wlb3.01025",
url = "conv_1628"
}

Corlatti, L., Iacolina, L., Safner, T., Apollonio, M., Buzan, E., Ferretti, F., Hammer, S. E., Herrero, J., Rossi, L., Serrano, E., Arnal, M., Brivio, F., Chirichella, R., Cotza, A., Crestanello, B., Espunyes, J., de Luco, D. F., Friedrich, S., Gačić, D., Grassi, L., Grignolio, S., Hauffe, H. C., Kavcić, K., Kinser, A., Lioce, F., Malagnino, A., Miller, C., Peters, W., Pokorny, B., Reiner, R., Rezić, A., Stipoljev, S., Tesija, T., Yankov, Y., Zwijacz-Kozica, T.,& Sprem, N.. (2022). Past, present and future of chamois science. in Wildlife Biology, 2022(4).
https://doi.org/10.1002/wlb3.01025
conv_1628

Corlatti L, Iacolina L, Safner T, Apollonio M, Buzan E, Ferretti F, Hammer SE, Herrero J, Rossi L, Serrano E, Arnal M, Brivio F, Chirichella R, Cotza A, Crestanello B, Espunyes J, de Luco DF, Friedrich S, Gačić D, Grassi L, Grignolio S, Hauffe HC, Kavcić K, Kinser A, Lioce F, Malagnino A, Miller C, Peters W, Pokorny B, Reiner R, Rezić A, Stipoljev S, Tesija T, Yankov Y, Zwijacz-Kozica T, Sprem N. Past, present and future of chamois science. in Wildlife Biology. 2022;2022(4).
doi:10.1002/wlb3.01025
conv_1628 .

Corlatti, L., Iacolina, L., Safner, Toni, Apollonio, M., Buzan, Elena, Ferretti, F., Hammer, S. E., Herrero, J., Rossi, L., Serrano, E., Arnal, MariaCruz, Brivio, F., Chirichella, R., Cotza, A., Crestanello, B., Espunyes, J., de Luco, D. Fernandez, Friedrich, S., Gačić, Dragan, Grassi, L., Grignolio, S., Hauffe, H. C., Kavcić, K., Kinser, A., Lioce, F., Malagnino, A., Miller, C., Peters, W., Pokorny, Bostjan, Reiner, R., Rezić, Andrea, Stipoljev, S., Tesija, T., Yankov, Y., Zwijacz-Kozica, T., Sprem, Nikica, "Past, present and future of chamois science" in Wildlife Biology, 2022, no. 4 (2022),
https://doi.org/10.1002/wlb3.01025 .,
conv_1628 .

TY  - JOUR
AU  - Segelbacher, Gernot
AU  - Bosse, Mirte
AU  - Burger, Pamela
AU  - Galbusera, Peter
AU  - Godoy, Jose A.
AU  - Helsen, Philippe
AU  - Hvilsom, Christina
AU  - Iacolina, Laura
AU  - Kahrić, Adla
AU  - Manfrin, Chiara
AU  - Nonić, Marina
AU  - Thizy, Delphine
AU  - Tsvetkov, Ivaylo
AU  - Velicković, Nevena
AU  - Vila, Carles
AU  - Wisely, Samantha M.
AU  - Buzan, Elena
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1328
AB  - Recent technological advances in the field of genomics offer conservation managers and practitioners new tools to explore for conservation applications. Many of these tools are well developed and used by other life science fields, while others are still in development. Considering these technological possibilities, choosing the right tool(s) from the toolbox is crucial and can pose a challenging task. With this in mind, we strive to inspire, inform and illuminate managers and practitioners on how conservation efforts can benefit from the current genomic and biotechnological revolution. With inspirational case studies we show how new technologies can help resolve some of the main conservation challenges, while also informing how implementable the different technologies are. We here focus specifically on small population management, highlight the potential for genetic rescue, and discuss the opportunities in the field of gene editing to help with adaptation to changing environments. In addition, we delineate potential applications of gene drives for controlling invasive species. We illuminate that the genomic toolbox offers added benefit to conservation efforts, but also comes with limitations for the use of these novel emerging techniques.
T2  - Conservation Genetics
T1  - New developments in the field of genomic technologies and their relevance to conservation management
EP  - 242
IS  - 2
SP  - 217
VL  - 23
DO  - 10.1007/s10592-021-01415-5
UR  - conv_1589
ER  - 

@article{
author = "Segelbacher, Gernot and Bosse, Mirte and Burger, Pamela and Galbusera, Peter and Godoy, Jose A. and Helsen, Philippe and Hvilsom, Christina and Iacolina, Laura and Kahrić, Adla and Manfrin, Chiara and Nonić, Marina and Thizy, Delphine and Tsvetkov, Ivaylo and Velicković, Nevena and Vila, Carles and Wisely, Samantha M. and Buzan, Elena",
year = "2022",
abstract = "Recent technological advances in the field of genomics offer conservation managers and practitioners new tools to explore for conservation applications. Many of these tools are well developed and used by other life science fields, while others are still in development. Considering these technological possibilities, choosing the right tool(s) from the toolbox is crucial and can pose a challenging task. With this in mind, we strive to inspire, inform and illuminate managers and practitioners on how conservation efforts can benefit from the current genomic and biotechnological revolution. With inspirational case studies we show how new technologies can help resolve some of the main conservation challenges, while also informing how implementable the different technologies are. We here focus specifically on small population management, highlight the potential for genetic rescue, and discuss the opportunities in the field of gene editing to help with adaptation to changing environments. In addition, we delineate potential applications of gene drives for controlling invasive species. We illuminate that the genomic toolbox offers added benefit to conservation efforts, but also comes with limitations for the use of these novel emerging techniques.",
journal = "Conservation Genetics",
title = "New developments in the field of genomic technologies and their relevance to conservation management",
pages = "242-217",
number = "2",
volume = "23",
doi = "10.1007/s10592-021-01415-5",
url = "conv_1589"
}

Segelbacher, G., Bosse, M., Burger, P., Galbusera, P., Godoy, J. A., Helsen, P., Hvilsom, C., Iacolina, L., Kahrić, A., Manfrin, C., Nonić, M., Thizy, D., Tsvetkov, I., Velicković, N., Vila, C., Wisely, S. M.,& Buzan, E.. (2022). New developments in the field of genomic technologies and their relevance to conservation management. in Conservation Genetics, 23(2), 217-242.
https://doi.org/10.1007/s10592-021-01415-5
conv_1589

Segelbacher G, Bosse M, Burger P, Galbusera P, Godoy JA, Helsen P, Hvilsom C, Iacolina L, Kahrić A, Manfrin C, Nonić M, Thizy D, Tsvetkov I, Velicković N, Vila C, Wisely SM, Buzan E. New developments in the field of genomic technologies and their relevance to conservation management. in Conservation Genetics. 2022;23(2):217-242.
doi:10.1007/s10592-021-01415-5
conv_1589 .

Segelbacher, Gernot, Bosse, Mirte, Burger, Pamela, Galbusera, Peter, Godoy, Jose A., Helsen, Philippe, Hvilsom, Christina, Iacolina, Laura, Kahrić, Adla, Manfrin, Chiara, Nonić, Marina, Thizy, Delphine, Tsvetkov, Ivaylo, Velicković, Nevena, Vila, Carles, Wisely, Samantha M., Buzan, Elena, "New developments in the field of genomic technologies and their relevance to conservation management" in Conservation Genetics, 23, no. 2 (2022):217-242,
https://doi.org/10.1007/s10592-021-01415-5 .,
conv_1589 .

TY  - JOUR
AU  - Rezić, Andrea
AU  - Iacolina, Laura
AU  - Buzan, Elena
AU  - Safner, Toni
AU  - Bego, Ferdinand
AU  - Gačić, Dragan
AU  - Maletić, Vladimir
AU  - Markov, Georgi
AU  - Milošević, Dragana
AU  - Papaioannou, Haritakis
AU  - Sprem, Nikica
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1344
AB  - The Balkan chamois (Rupicapra rupicapra balcanica) is widespread on the Balkan Peninsula, along mountain massifs from Croatia in the north to Greece in the south and Bulgaria in the east. Knowledge on the genetic structure of Balkan chamois populations is limited and restricted to local studies. Therefore, the main objective of this study was to use nuclear (16 microsatellites) and mitochondrial (partial 376 base pairs control region) markers to investigate the genetic structure of this chamois subspecies throughout its distribution range and to obtain information on the degree of connectivity of the different (sub)populations. We extracted DNA from bone, dried skin and muscle tissue and successfully genotyped 92 individuals of Balkan chamois and sequenced the partial control region in 44 individuals. The Bayesian analysis suggested 3 genetic clusters and assigned individuals from Serbia and Bulgaria to two separate clusters, while individuals from the other countries belonged to the same cluster. Thirty new haplotypes were obtained from partial mitochondrial DNA sequences, with private haplotypes in all analyzed populations and only two haplotypes shared among populations, indicating the possibility of past translocations. The subspecies genetic composition presented here provides the necessary starting point to assess the conservation status of the Balkan chamois and allows the development of conservation strategies necessary for its sustainable management and conservation.
T2  - Conservation Genetics
T1  - The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA
EP  - 539
IS  - 3
SP  - 527
VL  - 23
DO  - 10.1007/s10592-022-01434-w
UR  - conv_1616
ER  - 

@article{
author = "Rezić, Andrea and Iacolina, Laura and Buzan, Elena and Safner, Toni and Bego, Ferdinand and Gačić, Dragan and Maletić, Vladimir and Markov, Georgi and Milošević, Dragana and Papaioannou, Haritakis and Sprem, Nikica",
year = "2022",
abstract = "The Balkan chamois (Rupicapra rupicapra balcanica) is widespread on the Balkan Peninsula, along mountain massifs from Croatia in the north to Greece in the south and Bulgaria in the east. Knowledge on the genetic structure of Balkan chamois populations is limited and restricted to local studies. Therefore, the main objective of this study was to use nuclear (16 microsatellites) and mitochondrial (partial 376 base pairs control region) markers to investigate the genetic structure of this chamois subspecies throughout its distribution range and to obtain information on the degree of connectivity of the different (sub)populations. We extracted DNA from bone, dried skin and muscle tissue and successfully genotyped 92 individuals of Balkan chamois and sequenced the partial control region in 44 individuals. The Bayesian analysis suggested 3 genetic clusters and assigned individuals from Serbia and Bulgaria to two separate clusters, while individuals from the other countries belonged to the same cluster. Thirty new haplotypes were obtained from partial mitochondrial DNA sequences, with private haplotypes in all analyzed populations and only two haplotypes shared among populations, indicating the possibility of past translocations. The subspecies genetic composition presented here provides the necessary starting point to assess the conservation status of the Balkan chamois and allows the development of conservation strategies necessary for its sustainable management and conservation.",
journal = "Conservation Genetics",
title = "The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA",
pages = "539-527",
number = "3",
volume = "23",
doi = "10.1007/s10592-022-01434-w",
url = "conv_1616"
}

Rezić, A., Iacolina, L., Buzan, E., Safner, T., Bego, F., Gačić, D., Maletić, V., Markov, G., Milošević, D., Papaioannou, H.,& Sprem, N.. (2022). The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA. in Conservation Genetics, 23(3), 527-539.
https://doi.org/10.1007/s10592-022-01434-w
conv_1616

Rezić A, Iacolina L, Buzan E, Safner T, Bego F, Gačić D, Maletić V, Markov G, Milošević D, Papaioannou H, Sprem N. The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA. in Conservation Genetics. 2022;23(3):527-539.
doi:10.1007/s10592-022-01434-w
conv_1616 .

Rezić, Andrea, Iacolina, Laura, Buzan, Elena, Safner, Toni, Bego, Ferdinand, Gačić, Dragan, Maletić, Vladimir, Markov, Georgi, Milošević, Dragana, Papaioannou, Haritakis, Sprem, Nikica, "The Balkan chamois, an archipelago or a peninsula? Insights from nuclear and mitochondrial DNA" in Conservation Genetics, 23, no. 3 (2022):527-539,
https://doi.org/10.1007/s10592-022-01434-w .,
conv_1616 .