TY  - JOUR
AU  - Cerny, Martin
AU  - Berka, Miroslav
AU  - Dvorak, Milon
AU  - Milenković, Ivan
AU  - Saiz-Fernandez, Inigo
AU  - Brzobohaty, Bretislav
AU  - Durković, Jaroslav
PY  - 2022
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1295
AB  - Poplars are among the fastest-growing trees and significant resources in agriculture and forestry. However, rapid growth requires a large water consumption, and irrigation water provides a natural means for pathogen spread. That includes members of Phytophthora spp. that have proven to be a global enemy to forests. With the known adaptability to new hosts, it is only a matter of time for more aggressive Phytophthora species to become a threat to poplar forests and plantations. Here, the effects of artificial inoculation with two different representatives of aggressive species (P. cactorum and P. plurivora) were analyzed in the proteome of the Phytophthora-tolerant hybrid poplar clone T-14 [Populus tremula L. 70 x (Populus x canescens (Ait.) Sm. 23)]. Wood microcore samples were collected at the active necrosis borders to provide insight into the molecular processes underlying the observed tolerance to Phytophthora. The analysis revealed the impact of Phytophthora on poplar primary and secondary metabolism, including carbohydrate-active enzymes, amino acid biosynthesis, phenolic metabolism, and lipid metabolism, all of which were confirmed by consecutive metabolome and lipidome profiling. Modulations of enzymes indicating systemic response were confirmed by the analysis of leaf proteome, and sampling of wood microcores in distal locations revealed proteins with abundance correlating with proximity to the infection, including germin-like proteins, components of proteosynthesis, glutamate carboxypeptidase, and an enzyme that likely promotes anthocyanin stability. Finally, the identified Phytophthora-responsive proteins were compared to those previously found in trees with compromised defense against Phytophthora, namely, Quercus spp. and Castanea sativa. That provided a subset of candidate markers of Phytophthora tolerance, including certain ribosomal proteins, auxin metabolism enzymes, dioxygenases, polyphenol oxidases, trehalose-phosphate synthase, mannose-1-phosphate guanylyltransferase, and rhamnose biosynthetic enzymes. In summary, this analysis provided the first insight into the molecular mechanisms of hybrid poplar defense against Phytophthora and identified prospective targets for improving Phytophthora tolerance in trees.
T2  - Frontiers in Plant Science
T1  - Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar
VL  - 13
DO  - 10.3389/fpls.2022.1018272
UR  - conv_1666
ER  - 

@article{
author = "Cerny, Martin and Berka, Miroslav and Dvorak, Milon and Milenković, Ivan and Saiz-Fernandez, Inigo and Brzobohaty, Bretislav and Durković, Jaroslav",
year = "2022",
abstract = "Poplars are among the fastest-growing trees and significant resources in agriculture and forestry. However, rapid growth requires a large water consumption, and irrigation water provides a natural means for pathogen spread. That includes members of Phytophthora spp. that have proven to be a global enemy to forests. With the known adaptability to new hosts, it is only a matter of time for more aggressive Phytophthora species to become a threat to poplar forests and plantations. Here, the effects of artificial inoculation with two different representatives of aggressive species (P. cactorum and P. plurivora) were analyzed in the proteome of the Phytophthora-tolerant hybrid poplar clone T-14 [Populus tremula L. 70 x (Populus x canescens (Ait.) Sm. 23)]. Wood microcore samples were collected at the active necrosis borders to provide insight into the molecular processes underlying the observed tolerance to Phytophthora. The analysis revealed the impact of Phytophthora on poplar primary and secondary metabolism, including carbohydrate-active enzymes, amino acid biosynthesis, phenolic metabolism, and lipid metabolism, all of which were confirmed by consecutive metabolome and lipidome profiling. Modulations of enzymes indicating systemic response were confirmed by the analysis of leaf proteome, and sampling of wood microcores in distal locations revealed proteins with abundance correlating with proximity to the infection, including germin-like proteins, components of proteosynthesis, glutamate carboxypeptidase, and an enzyme that likely promotes anthocyanin stability. Finally, the identified Phytophthora-responsive proteins were compared to those previously found in trees with compromised defense against Phytophthora, namely, Quercus spp. and Castanea sativa. That provided a subset of candidate markers of Phytophthora tolerance, including certain ribosomal proteins, auxin metabolism enzymes, dioxygenases, polyphenol oxidases, trehalose-phosphate synthase, mannose-1-phosphate guanylyltransferase, and rhamnose biosynthetic enzymes. In summary, this analysis provided the first insight into the molecular mechanisms of hybrid poplar defense against Phytophthora and identified prospective targets for improving Phytophthora tolerance in trees.",
journal = "Frontiers in Plant Science",
title = "Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar",
volume = "13",
doi = "10.3389/fpls.2022.1018272",
url = "conv_1666"
}

Cerny, M., Berka, M., Dvorak, M., Milenković, I., Saiz-Fernandez, I., Brzobohaty, B.,& Durković, J.. (2022). Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar. in Frontiers in Plant Science, 13.
https://doi.org/10.3389/fpls.2022.1018272
conv_1666

Cerny M, Berka M, Dvorak M, Milenković I, Saiz-Fernandez I, Brzobohaty B, Durković J. Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar. in Frontiers in Plant Science. 2022;13.
doi:10.3389/fpls.2022.1018272
conv_1666 .

Cerny, Martin, Berka, Miroslav, Dvorak, Milon, Milenković, Ivan, Saiz-Fernandez, Inigo, Brzobohaty, Bretislav, Durković, Jaroslav, "Defense mechanisms promoting tolerance to aggressive Phytophthora species in hybrid poplar" in Frontiers in Plant Science, 13 (2022),
https://doi.org/10.3389/fpls.2022.1018272 .,
conv_1666 .

TY  - JOUR
AU  - Saiz-Fernandez, Inigo
AU  - Milenković, Ivan
AU  - Berka, Miroslav
AU  - Cerny, Martin
AU  - Tomsovsky, Michal
AU  - Brzobohaty, Bretislav
AU  - Kerchev, Pavel
PY  - 2020
UR  - https://omorika.sfb.bg.ac.rs/handle/123456789/1141
AB  - Phytophthora cinnamomi is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied Phytophthora-woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between P. cinnamomi and sweet chestnut (Castanea sativa), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of P. cinnamomi attack on stem tissues immediately bordering the infection site and away from it. P. cinnamomi led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA), indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in P. cinnamomi pathogenicity.
T2  - International Journal of Molecular Sciences
T1  - Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut ( Castanea sativa ) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It
IS  - 22
VL  - 21
DO  - 10.3390/ijms21228525
UR  - conv_1514
ER  - 

@article{
author = "Saiz-Fernandez, Inigo and Milenković, Ivan and Berka, Miroslav and Cerny, Martin and Tomsovsky, Michal and Brzobohaty, Bretislav and Kerchev, Pavel",
year = "2020",
abstract = "Phytophthora cinnamomi is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied Phytophthora-woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between P. cinnamomi and sweet chestnut (Castanea sativa), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of P. cinnamomi attack on stem tissues immediately bordering the infection site and away from it. P. cinnamomi led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA), indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in P. cinnamomi pathogenicity.",
journal = "International Journal of Molecular Sciences",
title = "Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut ( Castanea sativa ) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It",
number = "22",
volume = "21",
doi = "10.3390/ijms21228525",
url = "conv_1514"
}

Saiz-Fernandez, I., Milenković, I., Berka, M., Cerny, M., Tomsovsky, M., Brzobohaty, B.,& Kerchev, P.. (2020). Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut ( Castanea sativa ) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It. in International Journal of Molecular Sciences, 21(22).
https://doi.org/10.3390/ijms21228525
conv_1514

Saiz-Fernandez I, Milenković I, Berka M, Cerny M, Tomsovsky M, Brzobohaty B, Kerchev P. Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut ( Castanea sativa ) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It. in International Journal of Molecular Sciences. 2020;21(22).
doi:10.3390/ijms21228525
conv_1514 .

Saiz-Fernandez, Inigo, Milenković, Ivan, Berka, Miroslav, Cerny, Martin, Tomsovsky, Michal, Brzobohaty, Bretislav, Kerchev, Pavel, "Integrated Proteomic and Metabolomic Profiling of Phytophthora cinnamomi Attack on Sweet Chestnut ( Castanea sativa ) Reveals Distinct Molecular Reprogramming Proximal to the Infection Site and Away from It" in International Journal of Molecular Sciences, 21, no. 22 (2020),
https://doi.org/10.3390/ijms21228525 .,
conv_1514 .