Norway spruce ( picea abies karst.) Variability in progeny tests in Bosnia and Herzegovina

Abstract

Norway spruce is one of the most important economic species is Bosnia and Herzegovina. It is the species at the edge of its natural range; nevertheless it attains significant growth and yield results. The species is often used for afforestation purposes. In the previous period, 4 progeny tests of Norway spruce were established in Bosnia and Herzegovina. The offspring from 6 natural populations: Han Pijesak 1, Han Pijesak 2, Foca, Potoci, Olovo and Knezevo were build-in the progeny tests. In 2016, the samples of Norway spruce from 2 progeny tests: Srebrenica and Drinic were collected. In total, 360 samples were collected. DNA isolation was done according to Dumoline et al. (1990). For assessment of genetic differences among populations, co-dominant nSSR microsatellite system had been used. The number of effective alleles ranged from 7.78 in the population Potoci up to 15 in the population Knezevo, the average number of alleles was 13. The observed heterozygosity ranged from 0.61 for the population Han Pijesak 2 up to 0.68 for population Knezevo. The average observed heterozygosity was 0.65. Fixation index was in the range from -0.073 in the population Potoci, to 0.030 for the population Han Pijesak 2. The average value of Wright fixation index is -0.007. The average fixation index indicates the existence of a very small number of homozygotes. Concerning the variability among populations it has been concluded that the total level of genetic differentiation among populations was very low (FST= 0.026). The result of Nei's genetic distance shows that the populations Olovo and Potoci are separated from other populations. The results obtained by genetic markers, in addition with other, morphological and physiological markers will be the basis for the further investigation of Norway spruce adaptability and possibility for the transfer of genetic material in light of climate changes.