Micropropagation of dianthus deltoides l. Through shoot tip and nodal cuttings culture
Апстракт
Micropropagation (shoot tip and nodal cuttings culture) was used for the rapid propagation of the non-invasive, decorative, native plants of maiden pink (Dianthus deltoides L.) in order to preserve their genetic diversity. In vitro culture was successfully established on Murashige and Skoog medium (MS) using seeds as the initial material. In the shoot multiplication phase, the explants were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA). The highest multiplication rate was achieved on a medium containing 0.1 mgL(-1) of BAP and 0.1 mgL(-1) of NAA. The rooting was successful on a hormone-free medium (100%), and the highest percentage of microplant acclimatization (97%) was recorded in a 4: 1 mixture of peat and sand.
Кључне речи:
tissue culture / shoot regeneration / Maiden pink / hyperhydricity / Dianthus deltoidesИзвор:
Archives of Biological Sciences, 2013, 65, 1, 17-22Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Шумски засади у функцији повећања пошумљености Србије (RS-MESTD-Technological Development (TD or TR)-31041)
DOI: 10.2298/ABS1301017M
ISSN: 0354-4664
WoS: 000312046300003
Scopus: 2-s2.0-84873112492
Институција/група
Šumarski fakultetTY - JOUR AU - Marković, Marija AU - Popović, Marija AU - Vilotić, Dragica PY - 2013 UR - https://omorika.sfb.bg.ac.rs/handle/123456789/532 AB - Micropropagation (shoot tip and nodal cuttings culture) was used for the rapid propagation of the non-invasive, decorative, native plants of maiden pink (Dianthus deltoides L.) in order to preserve their genetic diversity. In vitro culture was successfully established on Murashige and Skoog medium (MS) using seeds as the initial material. In the shoot multiplication phase, the explants were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA). The highest multiplication rate was achieved on a medium containing 0.1 mgL(-1) of BAP and 0.1 mgL(-1) of NAA. The rooting was successful on a hormone-free medium (100%), and the highest percentage of microplant acclimatization (97%) was recorded in a 4: 1 mixture of peat and sand. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Micropropagation of dianthus deltoides l. Through shoot tip and nodal cuttings culture EP - 22 IS - 1 SP - 17 VL - 65 DO - 10.2298/ABS1301017M UR - conv_1054 ER -
@article{ author = "Marković, Marija and Popović, Marija and Vilotić, Dragica", year = "2013", abstract = "Micropropagation (shoot tip and nodal cuttings culture) was used for the rapid propagation of the non-invasive, decorative, native plants of maiden pink (Dianthus deltoides L.) in order to preserve their genetic diversity. In vitro culture was successfully established on Murashige and Skoog medium (MS) using seeds as the initial material. In the shoot multiplication phase, the explants were cultured on MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA). The highest multiplication rate was achieved on a medium containing 0.1 mgL(-1) of BAP and 0.1 mgL(-1) of NAA. The rooting was successful on a hormone-free medium (100%), and the highest percentage of microplant acclimatization (97%) was recorded in a 4: 1 mixture of peat and sand.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Micropropagation of dianthus deltoides l. Through shoot tip and nodal cuttings culture", pages = "22-17", number = "1", volume = "65", doi = "10.2298/ABS1301017M", url = "conv_1054" }
Marković, M., Popović, M.,& Vilotić, D.. (2013). Micropropagation of dianthus deltoides l. Through shoot tip and nodal cuttings culture. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 65(1), 17-22. https://doi.org/10.2298/ABS1301017M conv_1054
Marković M, Popović M, Vilotić D. Micropropagation of dianthus deltoides l. Through shoot tip and nodal cuttings culture. in Archives of Biological Sciences. 2013;65(1):17-22. doi:10.2298/ABS1301017M conv_1054 .
Marković, Marija, Popović, Marija, Vilotić, Dragica, "Micropropagation of dianthus deltoides l. Through shoot tip and nodal cuttings culture" in Archives of Biological Sciences, 65, no. 1 (2013):17-22, https://doi.org/10.2298/ABS1301017M ., conv_1054 .