Uticaj tipa eksplantata, sastava hranljive podloge i supstrata na ožiljavanje i aklimatizaciju vrste Dianthus deltoides L.

Abstract

U radu je ispitana mogućnost dobijanja ožiljenih in vitro biljaka Dianthus deltoides gajenjem nodusnih i vršnih reznica na podlogama za ožiljavanje. One su uspešno formirale izdanke i korenov sistem, a najbolji rezultati su dobijeni na MS podlozi bez hormona. Sastav hranljive podloge (koncentracije NSK-naftil sirćetne kiseline), kao ni tip eksplanta (nodusne ili vršne reznice) nisu imale značajnog uticaja na broj, dužinu i razgranatost korenova dobijenih in vitro biljaka. Međutim, prisutan je slabo izražen uticaj dužine korenova i razgranatosti korenovog sistema na aklimatizaciju ožiljenica. Sastav supstrata je takođe imao uticaja na aklimatizaciju i najbolji rezultati (96% preživljavanja) su dobijeni korišćenjem treseta i peska u zapreminskom odnosu 4:1.

The possibility of developing roots and shoots on Dianthus deltoides nodal and apical cuttings on rooting media was investigated in vitro conditions. The explants developed roots and shoots successfully, and the best results were achieved on MS medium without plant hormones. Number of roots per shoot, length of roots and ramification of roots were not significantly dependent on the medium (NAA-naphthaleneacetic acid concentration), or on the type of explant used (nodal or apical cuttings). The acclimatization rate of microplants was influenced by root numbers and ramification. The transfer to soil was successful, maximum survival rates (96%) were achieved on peat - sand (4:1) mixture.