Optimisation of a lawsonia inermis L. Micropropagation protocol and acclimatization in a hydroponic culture
Abstract
Lawsonia inermis is an important industrial and medicinal plant, cultivated mostly for dye production which is used in cosmetic industry. The objective of this study was establishing an efficient in vitro propagation system in order to obtain plants that will be acclimatized and grown hydroponically. The effect of different media on in vitro rooting were examined, followed by growing obtained microplants in a hydroponic culture, in a half-strength modified Hoagland nutrient solution. The highest rooting rate (79.2%) was recorded on the half-strength MS media containing 0.50 mg.L-1 IBA, with a high average number of roots (7.4). Those microplants acclimatized well in a hydroponic culture, where very rapid growth was recorded, and well developed roots and shoots were formed. After transplanting plants from hydroponic to soil, the survival rate was 100%. This is the first study reporting an acclimatization procedure in a hydroponic for the henna plant.
Keywords:
plant growth regulators / in vitro rooting / hydroponic / Henna / acclimatizationSource:
Glasnik Šumarskog fakulteta, 2020, 121, 91-95Publisher:
- Univerzitet u Beogradu - Šumarski fakultet, Beograd
Funding / projects:
- This work was supported by the Ministry of Education and Science of the Republic of Serbia.
Collections
Institution/Community
Šumarski fakultetTY - JOUR AU - Marković, Marija AU - Đunisijević-Bojović, Danijela AU - Milutinović, Marijana AU - Petrović, Jelena PY - 2020 UR - https://omorika.sfb.bg.ac.rs/handle/123456789/1111 AB - Lawsonia inermis is an important industrial and medicinal plant, cultivated mostly for dye production which is used in cosmetic industry. The objective of this study was establishing an efficient in vitro propagation system in order to obtain plants that will be acclimatized and grown hydroponically. The effect of different media on in vitro rooting were examined, followed by growing obtained microplants in a hydroponic culture, in a half-strength modified Hoagland nutrient solution. The highest rooting rate (79.2%) was recorded on the half-strength MS media containing 0.50 mg.L-1 IBA, with a high average number of roots (7.4). Those microplants acclimatized well in a hydroponic culture, where very rapid growth was recorded, and well developed roots and shoots were formed. After transplanting plants from hydroponic to soil, the survival rate was 100%. This is the first study reporting an acclimatization procedure in a hydroponic for the henna plant. PB - Univerzitet u Beogradu - Šumarski fakultet, Beograd T2 - Glasnik Šumarskog fakulteta T1 - Optimisation of a lawsonia inermis L. Micropropagation protocol and acclimatization in a hydroponic culture EP - 95 IS - 121 SP - 91 DO - 10.2298/GSF2021091M UR - conv_499 ER -
@article{ author = "Marković, Marija and Đunisijević-Bojović, Danijela and Milutinović, Marijana and Petrović, Jelena", year = "2020", abstract = "Lawsonia inermis is an important industrial and medicinal plant, cultivated mostly for dye production which is used in cosmetic industry. The objective of this study was establishing an efficient in vitro propagation system in order to obtain plants that will be acclimatized and grown hydroponically. The effect of different media on in vitro rooting were examined, followed by growing obtained microplants in a hydroponic culture, in a half-strength modified Hoagland nutrient solution. The highest rooting rate (79.2%) was recorded on the half-strength MS media containing 0.50 mg.L-1 IBA, with a high average number of roots (7.4). Those microplants acclimatized well in a hydroponic culture, where very rapid growth was recorded, and well developed roots and shoots were formed. After transplanting plants from hydroponic to soil, the survival rate was 100%. This is the first study reporting an acclimatization procedure in a hydroponic for the henna plant.", publisher = "Univerzitet u Beogradu - Šumarski fakultet, Beograd", journal = "Glasnik Šumarskog fakulteta", title = "Optimisation of a lawsonia inermis L. Micropropagation protocol and acclimatization in a hydroponic culture", pages = "95-91", number = "121", doi = "10.2298/GSF2021091M", url = "conv_499" }
Marković, M., Đunisijević-Bojović, D., Milutinović, M.,& Petrović, J.. (2020). Optimisation of a lawsonia inermis L. Micropropagation protocol and acclimatization in a hydroponic culture. in Glasnik Šumarskog fakulteta Univerzitet u Beogradu - Šumarski fakultet, Beograd.(121), 91-95. https://doi.org/10.2298/GSF2021091M conv_499
Marković M, Đunisijević-Bojović D, Milutinović M, Petrović J. Optimisation of a lawsonia inermis L. Micropropagation protocol and acclimatization in a hydroponic culture. in Glasnik Šumarskog fakulteta. 2020;(121):91-95. doi:10.2298/GSF2021091M conv_499 .
Marković, Marija, Đunisijević-Bojović, Danijela, Milutinović, Marijana, Petrović, Jelena, "Optimisation of a lawsonia inermis L. Micropropagation protocol and acclimatization in a hydroponic culture" in Glasnik Šumarskog fakulteta, no. 121 (2020):91-95, https://doi.org/10.2298/GSF2021091M ., conv_499 .